##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3203976_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2682104
Sequences flagged as poor quality	0
Sequence length	75
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.09226823419226	35.0	31.0	35.0	15.0	35.0
2	31.2659046778201	35.0	31.0	35.0	15.0	35.0
3	30.993440597381756	35.0	31.0	35.0	15.0	35.0
4	31.311094200672308	35.0	31.0	35.0	15.0	35.0
5	31.337955202333692	35.0	31.0	35.0	15.0	35.0
6	34.64662555963527	39.0	34.0	40.0	16.0	40.0
7	34.57396208349863	39.0	34.0	40.0	16.0	40.0
8	34.53045258498552	39.0	34.0	40.0	16.0	40.0
9	34.47989116007433	39.0	34.0	40.0	16.0	40.0
10	34.58204118855943	39.0	34.0	40.0	16.0	40.0
11	34.623922114876976	39.0	34.0	40.0	16.0	40.0
12	34.22804149279819	39.0	34.0	40.0	16.0	40.0
13	34.274461765837565	39.0	34.0	40.0	16.0	40.0
14	34.25206703394052	39.0	31.0	40.0	16.0	40.0
15	34.30351283917402	39.0	34.0	40.0	16.0	40.0
16	34.44088036854649	39.0	34.0	40.0	16.0	40.0
17	34.48599756012444	39.0	34.0	40.0	16.0	40.0
18	34.53075794227219	39.0	34.0	40.0	16.0	40.0
19	34.55284955393228	39.0	34.0	40.0	16.0	40.0
20	34.510441056722634	39.0	34.0	40.0	16.0	40.0
21	34.52754255614249	39.0	34.0	40.0	16.0	40.0
22	34.57296920626493	39.0	34.0	40.0	16.0	40.0
23	34.577101782779486	39.0	34.0	40.0	16.0	40.0
24	34.60033354411313	39.0	34.0	40.0	16.0	40.0
25	34.61211608498403	39.0	34.0	40.0	16.0	40.0
26	34.62531803390174	39.0	34.0	40.0	16.0	40.0
27	34.6143982485392	39.0	34.0	40.0	16.0	40.0
28	34.491292656809726	39.0	34.0	40.0	16.0	40.0
29	34.56569469341979	39.0	34.0	40.0	16.0	40.0
30	34.58044393505994	39.0	34.0	40.0	16.0	40.0
31	34.56576105922813	39.0	34.0	40.0	16.0	40.0
32	34.50531933139058	39.0	34.0	40.0	16.0	40.0
33	34.397952130118746	39.0	34.0	40.0	16.0	40.0
34	34.42169431162998	39.0	34.0	40.0	16.0	40.0
35	34.41710239423975	39.0	34.0	40.0	16.0	40.0
36	34.4218919176885	39.0	34.0	40.0	16.0	40.0
37	34.39487618675488	39.0	34.0	40.0	16.0	40.0
38	34.41019624891503	39.0	34.0	40.0	16.0	40.0
39	34.369314910980336	39.0	34.0	40.0	16.0	40.0
40	34.29373208496017	39.0	34.0	40.0	16.0	40.0
41	34.26564033311162	39.0	34.0	40.0	16.0	40.0
42	34.26108905545795	39.0	34.0	40.0	16.0	40.0
43	34.18915112911356	39.0	34.0	40.0	16.0	40.0
44	34.07616483178877	39.0	34.0	40.0	16.0	40.0
45	34.08041037931415	39.0	34.0	40.0	16.0	40.0
46	34.04976466236954	39.0	34.0	40.0	16.0	40.0
47	33.9742105451541	39.0	31.0	40.0	16.0	40.0
48	33.998353904248305	39.0	34.0	40.0	16.0	40.0
49	33.95048029457471	39.0	31.0	40.0	16.0	40.0
50	33.84983654623385	39.0	31.0	40.0	16.0	40.0
51	33.89257165270251	39.0	31.0	40.0	16.0	40.0
52	33.90864112651858	39.0	31.0	40.0	16.0	40.0
53	33.84579307886644	39.0	31.0	40.0	16.0	40.0
54	33.873646957761515	39.0	31.0	40.0	16.0	40.0
55	33.851483387668786	39.0	31.0	40.0	16.0	40.0
56	33.79208487068361	39.0	31.0	40.0	16.0	40.0
57	33.67377886912663	39.0	31.0	40.0	16.0	40.0
58	33.69300221020512	39.0	31.0	40.0	16.0	40.0
59	33.62962771018574	39.0	31.0	40.0	16.0	40.0
60	33.59818970479892	38.0	31.0	40.0	16.0	40.0
61	33.55571819735551	38.0	31.0	40.0	16.0	40.0
62	33.505684343336426	38.0	31.0	40.0	16.0	40.0
63	33.47235155683747	38.0	31.0	40.0	16.0	40.0
64	33.453706493111376	38.0	31.0	40.0	16.0	40.0
65	33.37320514044198	38.0	31.0	40.0	16.0	40.0
66	33.326765852479994	38.0	31.0	40.0	16.0	40.0
67	33.31098346671121	38.0	31.0	40.0	16.0	40.0
68	33.27459859871205	38.0	31.0	40.0	16.0	40.0
69	33.2326274447225	38.0	31.0	40.0	16.0	40.0
70	33.12254334656672	38.0	31.0	39.0	16.0	40.0
71	33.01681031011474	38.0	30.0	39.0	16.0	40.0
72	32.98308082013225	38.0	30.0	39.0	16.0	40.0
73	32.92802367096876	38.0	30.0	39.0	16.0	40.0
74	32.87533891303246	38.0	30.0	39.0	16.0	40.0
75	32.78281341812249	38.0	30.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	8476.0
3	2042.0
4	2327.0
5	2406.0
6	2300.0
7	2363.0
8	2656.0
9	2864.0
10	3033.0
11	3270.0
12	3644.0
13	4043.0
14	4588.0
15	5349.0
16	5985.0
17	6921.0
18	8447.0
19	16514.0
20	31162.0
21	42071.0
22	47763.0
23	51455.0
24	55624.0
25	57958.0
26	61628.0
27	64993.0
28	68052.0
29	72627.0
30	77887.0
31	84305.0
32	91946.0
33	102101.0
34	114063.0
35	133639.0
36	165701.0
37	228371.0
38	395629.0
39	647901.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.62822867050083	18.215564731872373	13.255096429004205	24.90111016862259
2	14.746631748806161	19.959255867781415	40.720158502429435	24.57395388098299
3	18.138073691400482	25.89616211750178	29.231976090412605	26.733788100685135
4	12.147105406800035	16.91809862704802	39.08890184720652	31.845894118945424
5	13.472109955467799	38.13327149133665	35.22048362032196	13.174134932873594
6	31.158523308566703	38.544441229721144	17.793754455457357	12.50328100625479
7	27.85149270870928	32.33677739565655	22.30334841601966	17.508381479614513
8	26.249280415673663	35.53736171304319	20.242279941419124	17.97107792986402
9	26.78181010132344	15.880256693998444	19.190493731786688	38.147439472891435
10	13.767549655046935	29.476895750500354	34.558727029227796	22.196827565224915
11	34.688811470397866	23.512138231776248	24.17221703558102	17.626833262244865
12	23.26147681074261	24.932702087614796	30.845336347882114	20.96048475376048
13	27.781547620823055	22.409645561842495	26.116362378192644	23.69244443914181
14	21.97315987746933	21.05086156241518	27.6707018072379	29.30527675287759
15	23.340444665829512	29.363179056442256	23.849336192779997	23.447040084948235
16	23.385707638480834	28.568728132838995	25.312702266578775	22.732861962101396
17	22.238175700867675	28.225937547537306	26.84202402293125	22.693862728663767
18	22.29779307588371	28.13093750279631	27.28015021043181	22.29111921088817
19	23.20305252891014	27.553629538601037	27.306211839660204	21.937106092828614
20	23.4777995185869	27.109761590154598	27.243537163361303	22.168901727897204
21	23.640619453980904	26.89407271306407	27.148499834458324	22.316807998496703
22	23.37418683242708	26.632039622624625	27.17888642647712	22.81488711847117
23	23.206967365918697	26.8025400953878	26.91662963106576	23.073862907627742
24	22.702661790892524	26.842061307093235	27.17299552888329	23.282281373130946
25	23.172218526947503	26.793181770729248	27.07161989244265	22.9629798098806
26	22.921333400941947	27.23634132009795	27.05789932083171	22.784425958128395
27	23.152532489418757	27.206215717213055	26.73017153697247	22.911080256395724
28	23.23513869752848	27.042995263549933	26.810016554437826	22.91184948448376
29	23.24641400930016	27.243611731685274	26.69672764367079	22.813246615343775
30	23.26975389470356	27.034745856238235	26.868234788807595	22.827265460250608
31	23.21811533035259	26.671523550168075	26.919239522404798	23.191121597074535
32	23.021553228361018	26.979229739040694	26.91442986550857	23.08478716708972
33	23.213276517391147	26.92748879678204	26.807147229663265	23.05208745616355
34	23.442090031590883	26.873986569494164	26.875254231474333	22.808669167440623
35	23.436534689383667	26.8894967866633	26.7933781812257	22.88059034272733
36	23.365648759332224	27.030868303391664	26.58174328810516	23.02173964917095
37	23.285608345391655	26.711986825263605	26.89300149919671	23.10940333014802
38	23.250324467960166	26.938204373242737	26.853083964963055	22.958387193834046
39	23.40079422929148	27.011103625260386	26.73971150185843	22.8483906435897
40	23.47665246263846	26.70479097931735	27.033823831523247	22.784732726520943
41	23.248370952641764	26.761155204021943	26.96700221357308	23.02347162976322
42	23.280473782249274	26.757071760830044	26.912772046727984	23.049682410192702
43	23.096847174343107	26.732018394527874	26.946253349052345	23.22488108207667
44	23.298320784642996	26.982766398989444	26.79805942091342	22.920853395454134
45	23.34017505103325	26.95323583419554	26.651417279531707	23.055171835239506
46	23.315834607210768	26.916334215726483	26.47056411021215	23.2972670668506
47	23.208774312088057	26.872430653271202	26.772546308827927	23.146248725812814
48	23.02016217882921	27.067176068313607	26.811480999410538	23.101180753446645
49	23.24563970884041	26.738980568606053	26.795167821668297	23.22021190088524
50	23.444388269472828	26.724658625194625	26.784089756667917	23.046863348664626
51	23.205442637473425	27.01595253239834	26.460637668796107	23.317967161332128
52	23.336263877206484	26.97107038674231	26.496404304693044	23.196261431358163
53	23.19379528188486	27.026779136255037	26.595324239920537	23.184101341939563
54	23.07565251045292	26.919798689498165	26.858128892113648	23.14641990793527
55	23.15623379272057	26.796172034014266	26.662431102167705	23.385163071097455
56	23.003361178481747	27.178492931831272	26.68171709055794	23.13642879912904
57	23.364586335501915	26.90932329240379	26.497107292114467	23.228983079979823
58	23.250118098595827	26.74945312986446	26.826967567347893	23.17346120419182
59	23.315921538799966	26.90588309862831	26.593216484159743	23.184978878411986
60	23.124005650941395	27.035630833498942	26.830972498080747	23.009391017478915
61	23.34090222704444	26.788514669858277	26.794368444797414	23.07621465829987
62	23.0682729546096	27.05288468529401	26.772389555275588	23.106452804820805
63	23.059637385368646	26.8255634814226	26.835965871213197	23.27883326199556
64	23.178544237298375	26.85855414014164	26.777198703383743	23.185702919176244
65	23.31293719004505	26.870784696591365	26.67839404513649	23.137884068227095
66	23.176904675261145	26.972720683422125	26.67138199693367	23.178992644383065
67	23.15178550122891	26.85130914882955	26.615481900398503	23.381423449543036
68	23.11367220853017	26.925813102859426	26.924097971297645	23.03641671731276
69	23.356284948018637	26.81394128826082	26.619050797179288	23.21072296654125
70	23.150321421420564	26.58754142907905	26.920577346771594	23.341559802728792
71	23.279430193439083	26.72819461952929	26.65410902848697	23.338266158544656
72	23.012845273586578	27.01866017219497	26.653644415743628	23.314850138474824
73	23.16953178625451	26.75017626199299	26.746373253365757	23.33391869838674
74	23.13759719259103	26.861992672758035	26.641489370428395	23.35892076422254
75	23.153236489753944	26.94480393220577	26.724898091278448	23.17706148676184
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	157.0
1	204.0
2	273.5
3	296.5
4	297.0
5	288.5
6	290.0
7	328.0
8	356.0
9	418.0
10	573.5
11	719.0
12	771.0
13	812.0
14	909.0
15	1132.0
16	1299.0
17	1499.0
18	2027.5
19	2764.0
20	3172.0
21	3966.0
22	6289.0
23	9629.5
24	11441.0
25	14143.5
26	20294.0
27	26999.5
28	30257.0
29	34845.5
30	43716.5
31	52516.5
32	57034.0
33	61522.0
34	70472.0
35	79838.0
36	84742.0
37	87903.0
38	94504.5
39	101193.5
40	104442.0
41	108100.5
42	113911.0
43	118987.5
44	121912.0
45	124887.5
46	129645.0
47	134168.0
48	136909.0
49	136833.0
50	135723.5
51	132218.5
52	129747.0
53	126204.5
54	118870.0
55	110070.5
56	105063.0
57	100592.5
58	88374.0
59	72575.0
60	64524.0
61	58993.0
62	46658.5
63	34442.0
64	29029.0
65	23976.5
66	15806.5
67	10792.0
68	8895.0
69	7254.5
70	4562.0
71	2839.5
72	2169.0
73	1987.0
74	1452.0
75	843.0
76	587.0
77	449.5
78	227.5
79	105.5
80	68.0
81	56.0
82	26.5
83	9.0
84	9.0
85	7.0
86	3.0
87	1.5
88	2.0
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.002945448796914661
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.09171903848620336
29	0.0
30	0.0
31	0.0
32	0.0
33	0.05223511094275241
34	3.728416198626153E-5
35	3.728416198626153E-5
36	0.0
37	3.728416198626153E-5
38	7.829674017114922E-4
39	2.609891339038307E-4
40	3.728416198626153E-5
41	5.59262429793923E-4
42	0.0010439565356153229
43	7.456832397252307E-5
44	6.711149157527076E-4
45	0.0010812406976015844
46	1.4913664794504613E-4
47	7.456832397252307E-5
48	1.1185248595878459E-4
49	3.728416198626153E-5
50	2.9827329589009226E-4
51	8.202515636977538E-4
52	2.9827329589009226E-4
53	5.965465917801845E-4
54	0.0029081646349283998
55	0.001976060585271861
56	3.355574578763538E-4
57	5.59262429793923E-4
58	8.575357256840152E-4
59	5.219782678076614E-4
60	0.002721743824997092
61	0.0027590279869833534
62	0.002274333881161953
63	0.0010439565356153229
64	0.0017150714513680305
65	0.002274333881161953
66	0.0026844596630108304
67	0.0020879130712306458
68	0.003504711226708584
69	0.0031691537688322306
70	0.00413854198047503
71	0.0026471755010245685
72	0.0010439565356153229
73	6.33830753766446E-4
74	0.0021624813952031688
75	0.001752355613354292
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	2682104.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.23313081153458
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.66744525106203	49.743888561696394
2	11.589021247565192	14.656201930498868
3	4.244055771273432	8.050948012691435
4	2.0187401863834524	5.106050491203462
5	1.0704382768449034	3.384358179270372
6	0.6410600775403247	2.432174144469591
7	0.4085987877170945	1.808588641520464
8	0.27595864384573016	1.395978321989659
9	0.19138564837394992	1.0891722365172303
>10	0.8385126842880168	9.000747757593691
>50	0.03996691175123373	1.6798259491165295
>100	0.014637363258809244	1.5917996353158048
>500	1.791500959249944E-4	0.06026613811644874
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	3.728416198626153E-5	0.0	0.0	0.0	0.0
9	3.728416198626153E-5	0.0	0.0	0.0	0.0
10	3.728416198626153E-5	0.0	0.0	0.0	0.0
11	3.728416198626153E-5	0.0	0.0	3.728416198626153E-5	0.0
12	3.728416198626153E-5	0.0	0.0	3.728416198626153E-5	0.0
13	3.728416198626153E-5	0.0	0.0	3.728416198626153E-5	0.0
14	3.728416198626153E-5	0.0	0.0	3.728416198626153E-5	0.0
15	3.728416198626153E-5	0.0	0.0	3.728416198626153E-5	0.0
16	3.728416198626153E-5	0.0	0.0	3.728416198626153E-5	0.0
17	3.728416198626153E-5	0.0	0.0	3.728416198626153E-5	0.0
18	3.728416198626153E-5	0.0	0.0	3.728416198626153E-5	0.0
19	3.728416198626153E-5	0.0	0.0	3.728416198626153E-5	0.0
20	7.456832397252307E-5	0.0	0.0	3.728416198626153E-5	3.728416198626153E-5
21	7.456832397252307E-5	0.0	0.0	3.728416198626153E-5	3.728416198626153E-5
22	7.456832397252307E-5	0.0	0.0	3.728416198626153E-5	3.728416198626153E-5
23	7.456832397252307E-5	0.0	0.0	3.728416198626153E-5	3.728416198626153E-5
24	7.456832397252307E-5	0.0	0.0	7.456832397252307E-5	3.728416198626153E-5
25	7.456832397252307E-5	0.0	0.0	1.1185248595878459E-4	3.728416198626153E-5
26	7.456832397252307E-5	0.0	0.0	1.4913664794504613E-4	3.728416198626153E-5
27	7.456832397252307E-5	0.0	0.0	1.8642080993130767E-4	3.728416198626153E-5
28	7.456832397252307E-5	0.0	0.0	3.355574578763538E-4	3.728416198626153E-5
29	7.456832397252307E-5	0.0	0.0	8.948198876702767E-4	3.728416198626153E-5
30	7.456832397252307E-5	0.0	0.0	0.0017150714513680305	3.728416198626153E-5
31	7.456832397252307E-5	0.0	0.0	0.004548667762323907	3.728416198626153E-5
32	7.456832397252307E-5	0.0	0.0	0.007866958179101184	3.728416198626153E-5
33	7.456832397252307E-5	0.0	0.0	0.011147964433892198	3.728416198626153E-5
34	7.456832397252307E-5	0.0	0.0	0.014913664794504613	3.728416198626153E-5
35	7.456832397252307E-5	0.0	0.0	0.018828501803062073	3.728416198626153E-5
36	7.456832397252307E-5	0.0	0.0	0.02341445372737224	3.728416198626153E-5
37	7.456832397252307E-5	0.0	0.0	0.029790045427022963	3.728416198626153E-5
38	7.456832397252307E-5	0.0	0.0	0.039260222571533394	3.728416198626153E-5
39	7.456832397252307E-5	0.0	0.0	0.051303006893095866	3.728416198626153E-5
40	7.456832397252307E-5	0.0	0.0	0.062115413869111714	3.728416198626153E-5
41	7.456832397252307E-5	0.0	0.0	0.07397177738074288	3.728416198626153E-5
42	7.456832397252307E-5	0.0	0.0	0.08657382413209928	3.728416198626153E-5
43	7.456832397252307E-5	0.0	0.0	0.09939957585537325	3.728416198626153E-5
44	7.456832397252307E-5	0.0	0.0	0.11312014746631749	3.728416198626153E-5
45	7.456832397252307E-5	0.0	0.0	0.12661701410534415	3.728416198626153E-5
46	7.456832397252307E-5	0.0	0.0	0.14261191959745037	3.728416198626153E-5
47	7.456832397252307E-5	0.0	0.0	0.15860682508955656	7.456832397252307E-5
48	7.456832397252307E-5	0.0	0.0	0.17613038122309949	7.456832397252307E-5
49	7.456832397252307E-5	0.0	0.0	0.19503345135013408	7.456832397252307E-5
50	7.456832397252307E-5	0.0	0.0	0.21289256494155334	7.456832397252307E-5
51	7.456832397252307E-5	0.0	0.0	0.2309753835048902	7.456832397252307E-5
52	7.456832397252307E-5	0.0	0.0	0.25148167259733406	7.456832397252307E-5
53	7.456832397252307E-5	0.0	0.0	0.27258450828155806	7.456832397252307E-5
54	7.456832397252307E-5	0.0	0.0	0.2964836561147517	7.456832397252307E-5
55	7.456832397252307E-5	0.0	0.0	0.3189660057924674	7.456832397252307E-5
56	7.456832397252307E-5	0.0	0.0	0.34431923594312525	7.456832397252307E-5
57	7.456832397252307E-5	0.0	0.0	0.3682183837763189	7.456832397252307E-5
58	1.1185248595878459E-4	0.0	0.0	0.3956595269982074	7.456832397252307E-5
59	1.1185248595878459E-4	0.0	0.0	0.42373450097386234	7.456832397252307E-5
60	1.1185248595878459E-4	0.0	0.0	0.45412109299266545	7.456832397252307E-5
61	1.1185248595878459E-4	0.0	0.0	0.4839857067436609	7.456832397252307E-5
62	1.1185248595878459E-4	0.0	0.0	0.5159382335658871	7.456832397252307E-5
63	1.1185248595878459E-4	0.0	0.0	0.5494566951915362	7.456832397252307E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2945	0.0	36.31152	1
TCAACGC	3375	0.0	30.04924	4
ATCAACG	3500	0.0	28.976055	3
CAACGCA	3585	0.0	28.577698	5
TATCAAC	3730	0.0	27.836693	2
AACGCAG	3695	0.0	27.540226	6
GGTATCA	1470	0.0	25.813292	1
CGTGCCG	375	0.0	23.916742	10
ACGCAGA	4375	0.0	23.101997	7
CGCAGAG	4480	0.0	21.636562	8
TCAATCG	245	1.8189894E-12	21.119572	45
GCACCGT	615	0.0	20.75327	6
GTACATG	6615	0.0	20.702782	1
TACATGG	6535	0.0	20.164047	2
ACATGGG	6885	0.0	18.78829	3
AGAGTAC	4705	0.0	18.768965	11
TCGCAAT	315	0.0	18.616858	49
GTATAAT	1180	0.0	18.417326	1
CAATCGC	375	0.0	18.397495	46
AATCGCA	320	0.0	18.325628	47
>>END_MODULE