FastQCFastQC Report
Mon 6 Feb 2017
SRR3203976_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203976_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2682104
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45550.041.9763571
GGTATCA18000.035.724541
TATCAAC61600.030.578562
ATCAACG60900.030.424833
TCAACGC60800.030.2403644
CAACGCA61850.029.9362745
AACGCAG63300.029.3050426
ACGCAGA74600.024.5807657
CGCAGAG77850.023.4238788
CGTACTA14250.023.35526829
GCGATAC2600.022.40497214
ACCGTAC15150.021.96782527
TATGCCG14900.021.95412343
GAGTACT51750.021.65119612
AGAGTAC79800.021.49092711
GACCGTA15500.021.47177926
CCGTACT15850.020.99763928
CAGAGTA84600.020.62232610
GTACATG66350.020.6205561
TCGTATG16850.020.54918140
Produced by FastQC (version 0.11.5)