##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3203976_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2682104
Sequences flagged as poor quality	0
Sequence length	75
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.63050388799241	35.0	32.0	35.0	27.0	35.0
2	32.63812626206888	35.0	32.0	35.0	27.0	35.0
3	32.655847797102574	35.0	32.0	35.0	27.0	35.0
4	32.77858725836135	35.0	32.0	35.0	30.0	35.0
5	32.86072874131652	35.0	33.0	35.0	30.0	35.0
6	36.38306046297981	39.0	36.0	40.0	27.0	40.0
7	36.18303540802295	39.0	36.0	40.0	27.0	40.0
8	36.0319577466049	39.0	36.0	40.0	27.0	40.0
9	36.011390684328425	39.0	36.0	40.0	27.0	40.0
10	35.946651211138715	39.0	36.0	40.0	27.0	40.0
11	36.01701611868891	39.0	36.0	40.0	27.0	40.0
12	36.036434828776216	39.0	36.0	40.0	27.0	40.0
13	36.02795305476596	39.0	36.0	40.0	27.0	40.0
14	35.96767947849897	39.0	36.0	40.0	27.0	40.0
15	35.94834801335071	39.0	36.0	40.0	27.0	40.0
16	35.943001091680266	39.0	36.0	40.0	27.0	40.0
17	35.68353016885251	39.0	35.0	40.0	27.0	40.0
18	35.64096843373709	39.0	35.0	40.0	27.0	40.0
19	35.82375478355798	39.0	36.0	40.0	27.0	40.0
20	35.803015468453125	39.0	36.0	40.0	27.0	40.0
21	35.60505185481249	39.0	35.0	40.0	27.0	40.0
22	35.61237446422659	39.0	35.0	40.0	27.0	40.0
23	35.410378195625526	39.0	35.0	40.0	27.0	40.0
24	35.469597002949925	39.0	35.0	40.0	27.0	40.0
25	35.245449840871196	39.0	35.0	40.0	16.0	40.0
26	35.294620566540296	39.0	35.0	40.0	27.0	40.0
27	35.38783469992215	39.0	35.0	40.0	27.0	40.0
28	35.371807357209114	39.0	35.0	40.0	27.0	40.0
29	35.33714501749373	39.0	35.0	40.0	27.0	40.0
30	35.26221913840776	39.0	35.0	40.0	16.0	40.0
31	35.29915320211297	39.0	35.0	40.0	16.0	40.0
32	35.08394342650397	39.0	34.0	40.0	16.0	40.0
33	35.301329851489726	39.0	35.0	40.0	27.0	40.0
34	35.31849510682658	39.0	35.0	40.0	27.0	40.0
35	35.36158329430924	39.0	35.0	40.0	27.0	40.0
36	35.34158145992848	39.0	35.0	40.0	27.0	40.0
37	35.156989810984214	39.0	35.0	40.0	16.0	40.0
38	35.19752589757891	39.0	35.0	40.0	16.0	40.0
39	35.21107123362852	39.0	35.0	40.0	16.0	40.0
40	35.26721969021335	39.0	35.0	40.0	27.0	40.0
41	35.376325079116995	39.0	35.0	40.0	27.0	40.0
42	35.13236846893334	39.0	35.0	40.0	16.0	40.0
43	35.118996877078594	39.0	35.0	40.0	16.0	40.0
44	35.161154451878076	39.0	35.0	40.0	16.0	40.0
45	34.97745762282149	39.0	34.0	40.0	16.0	40.0
46	34.9129325335632	39.0	34.0	40.0	16.0	40.0
47	34.98940645105484	39.0	34.0	40.0	16.0	40.0
48	34.882827809809015	39.0	34.0	40.0	16.0	40.0
49	34.89409321935317	39.0	34.0	40.0	16.0	40.0
50	34.81110799581224	39.0	34.0	40.0	16.0	40.0
51	34.54550681107071	39.0	34.0	40.0	16.0	40.0
52	34.79519101421869	39.0	34.0	40.0	16.0	40.0
53	34.79137162466481	39.0	34.0	40.0	16.0	40.0
54	34.73647479739786	39.0	34.0	40.0	16.0	40.0
55	34.656873111557196	39.0	34.0	40.0	16.0	40.0
56	34.67523742554353	39.0	34.0	40.0	16.0	40.0
57	34.628003612089614	39.0	34.0	40.0	16.0	40.0
58	34.66834544820037	39.0	34.0	40.0	16.0	40.0
59	34.620238066831114	39.0	34.0	40.0	16.0	40.0
60	34.359195989417266	39.0	34.0	40.0	16.0	40.0
61	34.563662333749924	39.0	34.0	40.0	16.0	40.0
62	34.307976499047015	39.0	34.0	40.0	16.0	40.0
63	34.46001534616107	39.0	34.0	40.0	16.0	40.0
64	34.43560316825895	39.0	34.0	40.0	16.0	40.0
65	34.39685672143958	39.0	34.0	40.0	16.0	40.0
66	34.39802185150166	39.0	34.0	40.0	16.0	40.0
67	34.37258771471949	39.0	34.0	40.0	16.0	40.0
68	34.293437912922094	39.0	34.0	40.0	16.0	40.0
69	34.181240921306554	39.0	34.0	40.0	16.0	40.0
70	34.10498437047929	39.0	34.0	40.0	16.0	40.0
71	34.05397963688209	39.0	34.0	40.0	16.0	40.0
72	34.05642585075001	39.0	34.0	40.0	16.0	40.0
73	33.90973877224746	39.0	34.0	40.0	16.0	40.0
74	33.83093720452302	39.0	34.0	40.0	16.0	40.0
75	33.7655929822259	39.0	34.0	40.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	739.0
3	1401.0
4	1217.0
5	1148.0
6	1527.0
7	2171.0
8	3120.0
9	4774.0
10	4375.0
11	3461.0
12	3735.0
13	4323.0
14	2548.0
15	3016.0
16	3529.0
17	4111.0
18	4847.0
19	6941.0
20	12777.0
21	21223.0
22	29096.0
23	37088.0
24	44235.0
25	50377.0
26	54188.0
27	59072.0
28	64531.0
29	69652.0
30	75805.0
31	82190.0
32	89234.0
33	98057.0
34	109913.0
35	126167.0
36	153758.0
37	208343.0
38	357614.0
39	881801.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.86561340779954	18.819324647100814	13.42843485493529	24.88662709016435
2	15.069288886635269	20.263009935483485	39.93226959133575	24.735431586545488
3	17.994042641438774	26.203836252458924	29.38083306043071	26.42128804567159
4	12.106761345472574	17.324266828012565	38.453951699932446	32.11502012658242
5	13.434520331793331	38.27733534772715	34.708547365783616	13.5795969546959
6	30.955921875460433	38.50950744507719	17.4772716167226	13.057299062739775
7	27.536926234031196	32.36585904200583	22.15238484413729	17.94482987982569
8	25.90563557544399	35.477742040740225	20.14502400596653	18.47159837784925
9	26.489705718407336	15.41590060935346	18.99991163386742	39.09448203837178
10	13.576963143087026	29.405369370739816	34.4975321594779	22.520135326695257
11	34.52889083761357	23.145853093998127	24.276312257279383	18.048943811108924
12	23.283749703586935	24.628197346226866	31.013344926377705	21.074708023808494
13	27.861568530072578	22.036831143613274	26.405533321551424	23.696067004762725
14	21.98723137548503	20.812678991350996	27.66969060525875	29.53039902790522
15	23.16331959855317	29.043821418277933	24.21757055092116	23.57528843224774
16	23.332821263524796	28.256223326072384	25.4590544258119	22.951900984590925
17	21.897574662990117	27.940491215712814	26.88632452261985	23.275609598677217
18	22.20418514936865	27.422196893036293	27.730858951087495	22.642759006507557
19	22.94149191259773	27.15904590506038	27.967748840421574	21.931713341920318
20	23.169532500687076	26.478538780773487	28.004837318502666	22.347091400036767
21	23.42223211163737	26.395775640749957	27.834585819451274	22.3474064281614
22	22.935991165159976	26.541169853389672	27.82828756910633	22.69455141234402
23	22.99294971277026	26.20718191066323	27.642314072487768	23.157554304078744
24	22.579263325061287	26.46687365548001	27.910263967051986	23.043599052406712
25	22.91144262334799	26.49377354605032	27.7430391515548	22.85174467904689
26	22.693021592214002	26.78861467216355	27.85245006682856	22.665913668793884
27	22.840418599561787	26.95455695731318	27.505312210582762	22.699712232542275
28	22.8344197979662	26.52686615728118	27.695423384608482	22.94329066014414
29	22.878178135794496	26.731800573224508	27.534104300988744	22.855916989992263
30	22.951301746266086	26.487830889182533	27.65939124738556	22.90147611716581
31	22.91533585756964	26.12169441980214	27.951217213476493	23.011752509151727
32	22.695732123305927	26.81504110649436	27.63408367682871	22.855143093371
33	22.94339766958709	26.415920626332685	27.639243052610702	23.001438651469527
34	23.0595062315712	26.662014119209935	27.69926292278716	22.579216726431707
35	23.02208396784414	26.592796644397705	27.721864369762482	22.663255017995674
36	23.212699592679133	26.56069126041486	27.298473609543056	22.92813553736295
37	22.983404614179868	26.461018914523223	27.670321064996084	22.885255406300825
38	22.981446100891127	26.458718204314362	27.58312743166703	22.976708263127477
39	23.221058598922895	26.68303371241491	27.24974392122222	22.84616376743998
40	23.095601835053493	26.26938257391031	27.99212345288153	22.642892138154664
41	22.918865959527402	26.483319029749588	27.517178323619984	23.08063668710303
42	23.17770657935355	26.30331406062762	27.59872263960742	22.92025672041141
43	22.96107261959845	26.23764232464906	27.68974182647998	23.111543229272513
44	23.01798311068297	26.712516511245692	27.383644833282013	22.885855544789326
45	23.060712456205295	26.505808757819484	27.291552155428672	23.14192663054655
46	23.36820205712508	26.38490255054195	26.9938962284046	23.25299916392837
47	23.00852163313229	26.513347068655424	27.190515897201955	23.28761540101033
48	22.86923835387789	26.67976851898591	27.104120465250364	23.346872661885836
49	23.180173912265357	26.335978332926928	27.226419273076264	23.257428481731456
50	23.298760199310674	26.53644011805921	27.24515332336084	22.919646359269276
51	23.014023447807894	26.78614242092962	26.6080597127455	23.591774418516984
52	23.07705538355693	26.746982545878762	27.0490644770494	23.126897593514904
53	23.050513729682354	26.77694683152979	27.08792538010463	23.084614058683226
54	22.79073263097439	26.65565117767767	27.164734282175996	23.388881909171946
55	23.016324386781083	26.513491434786246	27.165372190245108	23.304811988187566
56	22.9840178519829	26.903736127708044	27.03113843675838	23.081107583550672
57	23.22010155774321	26.727456645566903	26.666905292687865	23.38553650400202
58	22.939217132096807	26.736594609874764	27.18892510092089	23.135263157107538
59	22.992878448918717	26.910402684563756	27.01905294556301	23.077665920954512
60	23.07867609978102	27.03585328054941	26.938603903588486	22.946866716081082
61	23.126638446822323	26.84530452040744	27.06961164219584	22.958445390574404
62	22.86030146910802	27.169932413308608	26.96731104385806	23.002455073725315
63	22.829006533822714	26.949893439481233	27.1273700207752	23.093730005920857
64	22.969407202145636	26.97366526912232	27.024703743157946	23.032223785574097
65	23.120131986724854	26.84798471701091	26.961460011298794	23.07042328496544
66	22.945442233589304	27.002988271389032	26.920842503107966	23.130726991913694
67	22.940590810443965	26.903838552581533	26.903875841804204	23.251694795170298
68	22.94295136346077	26.939480627566326	27.159813442352526	22.95775456662038
69	23.12911484046217	26.847775116092443	26.801928978124735	23.22118106532065
70	22.86906558437298	26.658543215199604	27.079512967840508	23.392878232586913
71	23.142027960761848	26.641486467134467	26.879378192276484	23.3371073798272
72	22.888728323157572	26.893739273832107	26.893289323157948	23.324243079852373
73	23.08203472703631	26.577821100908878	26.9406191768746	23.39952499518022
74	23.031341418424727	26.75642775295557	26.843185354023287	23.36904547459642
75	23.100955664124072	26.791128720850192	26.914881908418163	23.19303370660757
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	90.0
1	93.5
2	168.5
3	264.5
4	289.0
5	256.0
6	264.5
7	416.5
8	527.0
9	994.0
10	1957.5
11	2463.0
12	2472.0
13	2372.5
14	2441.0
15	2821.0
16	3033.0
17	3360.0
18	4029.5
19	4843.5
20	5315.0
21	6238.5
22	8606.5
23	11766.5
24	13482.0
25	16120.5
26	22013.5
27	28063.5
28	30859.0
29	35334.5
30	44020.5
31	52634.5
32	57038.0
33	61059.0
34	69566.0
35	78323.0
36	82594.0
37	85780.5
38	92657.5
39	99814.0
40	103280.0
41	107267.0
42	114164.0
43	120200.0
44	123326.0
45	125792.5
46	129903.0
47	133254.0
48	134961.0
49	135588.0
50	134097.5
51	130300.5
52	128621.0
53	124922.0
54	117452.0
55	108635.0
56	103589.0
57	99325.5
58	87191.5
59	71350.0
60	63379.0
61	57894.5
62	45472.5
63	33220.0
64	27905.0
65	23031.5
66	15112.5
67	10382.5
68	8698.0
69	7052.0
70	4335.5
71	2615.5
72	1966.0
73	1812.0
74	1351.5
75	772.5
76	500.0
77	415.0
78	218.0
79	91.5
80	77.0
81	56.0
82	26.0
83	9.0
84	1.0
85	1.0
86	0.5
87	1.0
88	2.0
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08318096539134948
2	0.0
3	0.03914837008557461
4	0.049252377983851486
5	0.002721743824997092
6	0.04492741519344515
7	0.0
8	0.017859113591419274
9	0.003020017120887184
10	7.456832397252307E-5
11	2.9827329589009226E-4
12	0.0013422298315054152
13	9.693882116427998E-4
14	0.002274333881161953
15	0.003914837008557461
16	0.006636580833554553
17	0.06312208624274078
18	0.29760218097433955
19	0.019611469204773566
20	0.016889725379776475
21	0.3015915863068695
22	0.3036794993781002
23	0.9709541464462228
24	0.690278975013646
25	1.5089646039079767
26	0.971401556390058
27	0.8450455314186176
28	0.3092348395140531
29	0.848662095131285
30	0.8511974181463507
31	0.3091602711900806
32	0.9722218079537557
33	0.560119965519607
34	0.3094585444859707
35	0.30304566862433374
36	0.8949317401562357
37	0.9673002985715692
38	0.8449709630946451
39	0.5576219266665274
40	0.841242546896019
41	0.2963718036287929
42	0.8410188419241014
43	0.564407644148027
44	0.8408697052761563
45	0.28686434232229624
46	0.28678977399832367
47	0.28835570880174666
48	0.2860813749205847
49	0.002311618043148215
50	0.002237049719175692
51	1.0963034990440341
52	0.2857458174627084
53	0.28496285006099686
54	0.28664063735037865
55	0.5497549684874263
56	0.001826923937326815
57	0.003094585444859707
58	0.28828114047777414
59	0.0038775528465711992
60	0.856081643366551
61	0.0024980388530795226
62	0.5431183876538718
63	0.002237049719175692
64	0.28526112335688697
65	0.5429692510059267
66	0.010663270328070799
67	0.013571434962999198
68	0.009395608820537905
69	0.29611081449488913
70	0.5566152542928984
71	0.023563590375317287
72	0.564668633281931
73	0.016666020407858907
74	0.8564544849864136
75	0.06595568255369666
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	2682104.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.13486289001837
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.92014574979395	45.6544755525407
2	12.991731552082522	15.625119911768163
3	4.837414881776887	8.726918419733625
4	2.2497765290773866	5.411600124370003
5	1.2056275153127838	3.6250122664883886
6	0.7520254540999005	2.7133768543260794
7	0.47459245290319885	1.9977686458779953
8	0.3100551025822326	1.4916096865706514
9	0.23392428758850628	1.2660304464702046
>10	0.9617257632141172	9.745142724722642
>50	0.04552217224276387	1.859053996306002
>100	0.01708055954285573	1.7349356611176452
>500	3.149831523945466E-4	0.10739812742534846
>1k	6.299663047048632E-5	0.041557582282558056
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.728416198626153E-5	0.0
11	0.0	0.0	0.0	7.456832397252307E-5	0.0
12	0.0	0.0	0.0	7.456832397252307E-5	0.0
13	0.0	0.0	0.0	7.456832397252307E-5	0.0
14	0.0	0.0	0.0	7.456832397252307E-5	0.0
15	0.0	0.0	0.0	7.456832397252307E-5	0.0
16	0.0	0.0	0.0	7.456832397252307E-5	0.0
17	0.0	0.0	0.0	7.456832397252307E-5	0.0
18	0.0	0.0	0.0	7.456832397252307E-5	0.0
19	0.0	0.0	0.0	7.456832397252307E-5	0.0
20	0.0	0.0	0.0	1.1185248595878459E-4	0.0
21	0.0	0.0	0.0	1.1185248595878459E-4	0.0
22	0.0	0.0	0.0	1.1185248595878459E-4	0.0
23	0.0	0.0	0.0	1.1185248595878459E-4	0.0
24	0.0	0.0	0.0	1.4913664794504613E-4	0.0
25	0.0	0.0	0.0	1.8642080993130767E-4	0.0
26	0.0	0.0	0.0	2.609891339038307E-4	0.0
27	0.0	0.0	0.0	2.9827329589009226E-4	0.0
28	0.0	0.0	0.0	4.4740994383513837E-4	0.0
29	0.0	0.0	0.0	0.0010066723736290614	0.0
30	0.0	0.0	0.0	0.0017896397753405535	0.0
31	0.0	0.0	0.0	0.004287678628420076	0.0
32	0.0	0.0	0.0	0.007494116559238568	0.0
33	0.0	0.0	0.0	0.010588702004098276	0.0
34	0.0	0.0	0.0	0.014428970688683212	0.0
35	0.0	0.0	0.0	0.018269239373268152	0.0
36	0.0	0.0	0.0	0.022817907135592057	0.0
37	0.0	0.0	0.0	0.029118930511270257	0.0
38	0.0	0.0	0.0	0.03881281262769826	0.0
39	0.0	0.0	0.0	0.05048275532939812	0.0
40	0.0	0.0	0.0	0.06066133155164751	0.0
41	0.0	0.0	0.0	0.07180929598553971	0.0
42	0.0	0.0	0.0	0.08336738620128079	0.0
43	0.0	0.0	0.0	0.09582029630469213	0.0
44	0.0	0.0	0.0	0.10905617380981499	0.0
45	0.0	0.0	0.0	0.12158365223719886	0.0
46	0.0	0.0	0.0	0.13687015865156607	0.0
47	0.0	0.0	0.0	0.15234308587586462	0.0
48	0.0	0.0	0.0	0.16900910628372354	0.0
49	0.0	0.0	0.0	0.18664451490322523	0.0
50	0.0	0.0	0.0	0.2038325135788918	0.0
51	0.0	0.0	0.0	0.22120693306448966	0.0
52	0.0	0.0	0.0	0.24033370816344182	0.0
53	0.0	0.0	0.0	0.26001974569218794	0.0
54	0.0	0.0	0.0	0.281980117102096	0.0
55	0.0	0.0	0.0	0.30308295278631997	0.0
56	0.0	0.0	0.0	0.32675839564759607	0.0
57	0.0	0.0	0.0	0.3480476521417514	0.0
58	0.0	0.0	0.0	0.3740347130461757	0.0
59	0.0	0.0	0.0	0.4003946155704626	0.0
60	0.0	0.0	0.0	0.42925255694782904	0.0
61	0.0	0.0	0.0	0.4571783942755389	0.0
62	0.0	0.0	0.0	0.4878259754282459	0.0
63	0.0	0.0	0.0	0.5198903547364307	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4555	0.0	41.976357	1
GGTATCA	1800	0.0	35.72454	1
TATCAAC	6160	0.0	30.57856	2
ATCAACG	6090	0.0	30.42483	3
TCAACGC	6080	0.0	30.240364	4
CAACGCA	6185	0.0	29.936274	5
AACGCAG	6330	0.0	29.305042	6
ACGCAGA	7460	0.0	24.580765	7
CGCAGAG	7785	0.0	23.423878	8
CGTACTA	1425	0.0	23.355268	29
GCGATAC	260	0.0	22.404972	14
ACCGTAC	1515	0.0	21.967825	27
TATGCCG	1490	0.0	21.954123	43
GAGTACT	5175	0.0	21.651196	12
AGAGTAC	7980	0.0	21.490927	11
GACCGTA	1550	0.0	21.471779	26
CCGTACT	1585	0.0	20.997639	28
CAGAGTA	8460	0.0	20.622326	10
GTACATG	6635	0.0	20.620556	1
TCGTATG	1685	0.0	20.549181	40
>>END_MODULE