FastQCFastQC Report
Mon 6 Feb 2017
SRR3203975_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203975_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980961
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29750.034.5542371
TCAACGC34950.028.0294134
CAACGCA35250.027.8887215
ATCAACG36350.027.1396663
GGTATCA15500.026.0391121
AACGCAG38450.025.7471076
TATCAAC39450.025.5316412
TGCGATA3300.024.04123513
GCGATAC3400.022.3196114
GTACATG64600.021.7337131
ACGCAGA44200.021.6172477
TACATGG66400.020.7275352
CGCAGAG46750.020.5119088
ACATGGG68550.019.1213683
AGAGTAC46150.019.05949211
GTGCGAT4200.018.88954212
TCACGTC5400.017.8857546
CGATACT4250.017.8556915
GTATAGG7300.017.4844061
TCGCAAT3205.456968E-1217.24697349
Produced by FastQC (version 0.11.5)