FastQCFastQC Report
Mon 6 Feb 2017
SRR3203975_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203975_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2980961
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46500.040.5434231
GGTATCA20850.035.709581
TATCAAC60600.030.6985152
ATCAACG60900.030.380163
TCAACGC61250.030.249084
CAACGCA62000.029.8166125
AACGCAG64650.028.700436
ACGCAGA75900.024.1646147
CGCAGAG77550.023.5625538
AGAGTAC79300.022.06491311
GTACATG62400.021.7837561
CGTACTA11900.021.26688629
GAGTACT56300.021.22070512
CGTGCCG3400.021.0530110
CAGAGTA87450.020.4242110
TACATGG64200.020.3105562
GCAGAGT87500.020.2963549
ATGCGCG850.004183931720.24164653
AGTACTT60150.020.03452913
ACCGTAC13000.019.73371927
Produced by FastQC (version 0.11.5)