FastQCFastQC Report
Mon 6 Feb 2017
SRR3203974_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203974_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3254377
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA31550.032.6916851
TCAACGC38200.025.6452624
CAACGCA38600.025.4688725
ATCAACG38200.025.4646633
TATCAAC40950.024.3442332
GGTATCA17250.024.1970141
AACGCAG41500.023.2735236
GTACATG70200.022.1617971
TACATGG72450.021.0919882
ACATGGG74050.019.7511773
ACGCAGA47550.019.7319767
CGCAGAG48900.018.8345228
CGAATCG2255.5551936E-918.39716537
CATGGGG50800.018.333794
AGAGTAC51600.016.91309211
GTATAAG9300.016.6914881
ATGGGGG25950.016.2171545
CGATCGT1959.005629E-615.92062542
GTATTAC6750.015.8424891
CGCGTAT1350.00324708215.33167766
Produced by FastQC (version 0.11.5)