FastQCFastQC Report
Mon 6 Feb 2017
SRR3203974_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203974_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3254377
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47950.038.4785081
GGTATCA19700.034.8612481
ATCAACG61800.029.1705283
TCAACGC61950.028.978974
TATCAAC63050.028.7537782
CAACGCA63000.028.3762865
AACGCAG66800.026.762486
ACGCAGA76750.023.1471317
CTCGTAT17300.023.04249639
CGCAGAG78050.022.5868428
TATGCCG18750.021.68540643
TCGTATG19250.020.8877440
CCGTCTT19450.020.84758847
AGAGTAC81850.020.29746211
GCCGTCT20000.019.9277146
ATGCCGT20450.019.88239944
TCTCGTA16450.019.80795938
GAGTACT58750.019.52392812
TGCCGTC20750.019.3744545
GTACATG67200.019.2700581
Produced by FastQC (version 0.11.5)