FastQCFastQC Report
Mon 6 Feb 2017
SRR3203973_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203973_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3072142
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32050.036.4876671
ATCAACG39700.027.9776693
TCAACGC39400.027.928054
CAACGCA39500.027.770025
AACGCAG41850.026.128236
TATCAAC44350.025.7442682
GGTATCA18300.023.3746451
ACGCAGA48050.022.2543287
GTACATG72900.020.6316221
CGCAGAG52150.020.1739858
TACATGG74000.019.437952
AGAGTAC53700.018.82086211
GTATAGG9650.018.588761
GTATAGC11000.018.5026421
ACATGGG73000.018.23943
CCTATAC7850.016.6978323
CAGAGTA61350.016.47400710
CATGGGG53500.016.4411644
TATACTG13250.016.1406845
GCAGAGT63500.016.1335099
Produced by FastQC (version 0.11.5)