##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3203973_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3072142 Sequences flagged as poor quality 0 Sequence length 75 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.149980046495248 35.0 31.0 35.0 15.0 35.0 2 31.332889560443494 35.0 31.0 35.0 15.0 35.0 3 31.0722967883646 35.0 31.0 35.0 15.0 35.0 4 31.384542120774366 35.0 31.0 35.0 15.0 35.0 5 31.356187311654214 35.0 31.0 35.0 15.0 35.0 6 34.75596863686639 39.0 34.0 40.0 16.0 40.0 7 34.590403373281575 39.0 34.0 40.0 16.0 40.0 8 34.457751627366186 39.0 34.0 40.0 16.0 40.0 9 34.4798140841146 39.0 34.0 40.0 16.0 40.0 10 34.61313734846892 39.0 34.0 40.0 16.0 40.0 11 34.63351531276874 39.0 34.0 40.0 16.0 40.0 12 34.247632759162826 39.0 34.0 40.0 16.0 40.0 13 34.294287829143315 39.0 34.0 40.0 16.0 40.0 14 34.26190814096484 39.0 31.0 40.0 16.0 40.0 15 34.30307615989105 39.0 34.0 40.0 16.0 40.0 16 34.4321802182321 39.0 34.0 40.0 16.0 40.0 17 34.46984384185367 39.0 34.0 40.0 16.0 40.0 18 34.51225236333477 39.0 34.0 40.0 16.0 40.0 19 34.543936771151856 39.0 34.0 40.0 16.0 40.0 20 34.50139153724014 39.0 34.0 40.0 16.0 40.0 21 34.527189498402095 39.0 34.0 40.0 16.0 40.0 22 34.569171607302 39.0 34.0 40.0 16.0 40.0 23 34.58087581889119 39.0 34.0 40.0 16.0 40.0 24 34.610833744013135 39.0 34.0 40.0 16.0 40.0 25 34.62281626305034 39.0 34.0 40.0 16.0 40.0 26 34.62531484547264 39.0 34.0 40.0 16.0 40.0 27 34.61572186441903 39.0 34.0 40.0 16.0 40.0 28 34.49623552557141 39.0 34.0 40.0 16.0 40.0 29 34.571472282205704 39.0 34.0 40.0 16.0 40.0 30 34.58734947798637 39.0 34.0 40.0 16.0 40.0 31 34.56998244221784 39.0 34.0 40.0 16.0 40.0 32 34.51451886013081 39.0 34.0 40.0 16.0 40.0 33 34.40155435523488 39.0 34.0 40.0 16.0 40.0 34 34.432599795191756 39.0 34.0 40.0 16.0 40.0 35 34.41429823230827 39.0 34.0 40.0 16.0 40.0 36 34.42184280544324 39.0 34.0 40.0 16.0 40.0 37 34.399190206702684 39.0 34.0 40.0 16.0 40.0 38 34.403569887069025 39.0 34.0 40.0 16.0 40.0 39 34.36128668531598 39.0 34.0 40.0 16.0 40.0 40 34.27841388842052 39.0 34.0 40.0 16.0 40.0 41 34.24379602244948 39.0 34.0 40.0 16.0 40.0 42 34.250198070271495 39.0 34.0 40.0 16.0 40.0 43 34.18757629041887 39.0 34.0 40.0 16.0 40.0 44 34.06394854144112 39.0 34.0 40.0 16.0 40.0 45 34.07856114723864 39.0 34.0 40.0 16.0 40.0 46 34.03762586495025 39.0 34.0 40.0 16.0 40.0 47 33.97017650876815 39.0 31.0 40.0 16.0 40.0 48 33.99199516168198 39.0 31.0 40.0 16.0 40.0 49 33.93296826774283 39.0 31.0 40.0 16.0 40.0 50 33.82900237033314 39.0 31.0 40.0 16.0 40.0 51 33.85980530847858 39.0 31.0 40.0 16.0 40.0 52 33.88125418681819 39.0 31.0 40.0 16.0 40.0 53 33.81421301489319 39.0 31.0 40.0 16.0 40.0 54 33.84205547790434 39.0 31.0 40.0 16.0 40.0 55 33.82045784342 39.0 31.0 40.0 16.0 40.0 56 33.76779621514891 39.0 31.0 40.0 16.0 40.0 57 33.65292977993856 39.0 31.0 40.0 16.0 40.0 58 33.65543129191294 39.0 31.0 40.0 16.0 40.0 59 33.58872311240822 39.0 31.0 40.0 16.0 40.0 60 33.552859861295474 39.0 31.0 40.0 16.0 40.0 61 33.51631727960491 39.0 31.0 40.0 16.0 40.0 62 33.46455795337585 38.0 31.0 40.0 16.0 40.0 63 33.42154854821164 38.0 31.0 40.0 16.0 40.0 64 33.3962525169735 38.0 31.0 40.0 16.0 40.0 65 33.320782372689806 38.0 31.0 40.0 16.0 40.0 66 33.27463118566785 38.0 31.0 40.0 16.0 40.0 67 33.25794022541927 38.0 31.0 40.0 16.0 40.0 68 33.214341980286065 38.0 31.0 40.0 16.0 40.0 69 33.17364399171653 38.0 31.0 40.0 16.0 40.0 70 33.06542926726694 38.0 30.0 40.0 16.0 40.0 71 32.96485579117111 38.0 30.0 40.0 16.0 40.0 72 32.93037952021749 38.0 30.0 40.0 16.0 40.0 73 32.87771691542904 38.0 30.0 39.0 16.0 40.0 74 32.82625705452417 38.0 30.0 39.0 16.0 40.0 75 32.735678559129106 38.0 30.0 39.0 16.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7922.0 3 2404.0 4 2754.0 5 2838.0 6 2636.0 7 2794.0 8 3026.0 9 3323.0 10 3667.0 11 3968.0 12 4436.0 13 4967.0 14 5710.0 15 6397.0 16 7451.0 17 7975.0 18 9917.0 19 20155.0 20 39192.0 21 51721.0 22 57000.0 23 60622.0 24 64316.0 25 66807.0 26 69396.0 27 73160.0 28 76663.0 29 81260.0 30 87425.0 31 94188.0 32 103000.0 33 113903.0 34 127550.0 35 147829.0 36 184099.0 37 253965.0 38 445117.0 39 772589.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.31931484709548 17.830381687589416 13.185742908156373 24.664560557158737 2 14.903152263144086 19.280684291286015 41.24457788735026 24.57158555821964 3 19.081832805905456 24.698565365793637 28.385243911251496 27.834357917049406 4 11.961816869142117 16.134866161785492 38.85666743269029 33.046649536382105 5 13.604970082763101 37.83187756295119 34.91768935159898 13.645463002686725 6 32.05248976121546 37.47424435459038 17.621581294093826 12.851684590100326 7 28.231051819870306 31.845207675947268 21.77555594760919 18.148184556573234 8 25.486094067266425 36.70504162893512 19.584023134347305 18.22484116945115 9 25.853785404450708 16.438270106004214 19.044985550798106 38.662958938746975 10 13.526262783426027 29.94490489046405 34.41038858229861 22.118443743811323 11 35.385050560813916 23.38820926897259 23.38606093077729 17.8406792394362 12 23.15703505892631 24.813468908663726 30.88324042313148 21.146255609278477 13 27.98080297069602 22.184781823236037 25.769088798629753 24.0653264074382 14 21.500633759767616 21.040661531921376 27.645434358177457 29.813270350133553 15 23.33502162334944 29.583756219601824 23.591552734216062 23.48966942283267 16 23.369329933316884 28.662672493654263 25.153069096415464 22.814928476613385 17 22.22479371877788 28.37115874564351 26.689465099420893 22.714582436157716 18 22.29844844411489 28.09147493833293 27.30583417042571 22.30424244712647 19 23.205209915427087 27.588080238478558 27.204927376403827 22.00178246969053 20 23.25087837736667 27.161602556131847 27.329107834208184 22.258411232293298 21 23.51604304620133 26.83330615359126 27.28813553804985 22.362515262157565 22 22.96043703723234 26.856872780253248 27.287972785103285 22.89471739741112 23 22.989797668791894 26.86686581117208 26.988897970503306 23.15443854953272 24 22.608102099447226 26.826494348243017 27.369958810497693 23.195444741812064 25 22.987095969878986 26.769995257379136 27.257212478203314 22.985696294538567 26 22.791687363409636 27.032441859783823 27.27347889518128 22.902391881625263 27 23.033928770219607 27.237412853963132 26.83401353192658 22.89464484389068 28 22.908016142468657 27.16341431391413 26.95806600532544 22.970503538291776 29 23.09307317174792 27.243662565076743 26.900774768874612 22.76248949430072 30 23.046753698234003 27.163392837961265 27.027136115452997 22.762717348351735 31 23.14717976811611 26.84079278913305 26.976170690082256 23.03585675266858 32 22.77294564279439 27.05598473507564 27.207019469483985 22.96405015264599 33 22.96781892122242 26.910882840785522 27.085738590491353 23.03555964750071 34 23.084940547898743 27.01757766662099 27.15770813606294 22.739773649417327 35 23.220571471724252 26.967440915740116 26.995694854853525 22.81629275768211 36 22.999042036156546 27.142015942627634 27.004489702783825 22.854452318432 37 23.081179700761847 26.76041254697466 27.079767002426653 23.07864074983684 38 22.89579474693289 27.002874246039365 27.15755620729726 22.94377479973048 39 23.19110325555355 27.188551284367385 26.938204213031003 22.68214124704806 40 23.306244706455242 26.86288832077205 27.204409437336473 22.626457535436234 41 22.9946922680805 26.940596760154705 27.064778026393483 22.99993294537131 42 23.040626228799546 26.96839039303357 27.10520216763495 22.885781210531935 43 22.845856158107534 26.847012356545296 27.172648876010413 23.134482609336757 44 22.98962444561989 27.13177360947227 27.097888269520283 22.780713675387556 45 23.139365732553284 27.11318048078341 26.860845848343367 22.886607938319937 46 23.09493111138092 26.988056832174912 26.81531040485552 23.10170165158865 47 23.025770994513266 27.01449414999857 26.874786793293005 23.084948062195163 48 22.79407265052413 27.22249564797346 27.056975416420908 22.9264562850815 49 23.095544536085296 26.928673126011915 26.91972170520986 23.056060632692933 50 23.207514004941196 27.065879373594214 26.90247483016669 22.824131791297894 51 23.04573686483946 27.174290562501362 26.73843476664463 23.04153780601455 52 23.116632434174335 27.261769521472072 26.67898168371781 22.942616360635782 53 23.061889653722222 27.27199932034104 26.77364785137603 22.892463174560703 54 22.911025768393696 27.147078603333664 27.056650623132768 22.885245005139875 55 22.927626214440775 26.980954027107902 26.93460126369872 23.156818494752603 56 22.772593711132387 27.428000209626752 26.97544730409908 22.823958775141783 57 23.116955489455837 27.155201316612665 26.69809115902147 23.029752034910025 58 22.942087510961493 27.062502156492684 27.000297514613695 22.995112817932124 59 23.15204038386546 27.19583036849496 26.75815186786445 22.893977379775126 60 22.932730133112678 27.235805041504733 27.06627704741348 22.76518777796911 61 23.187585406804942 26.993953898065858 26.978947690508846 22.83951300462035 62 22.935810874593678 27.311253164795946 26.953319544620935 22.799616415989437 63 22.931763511072194 26.981227885720237 27.087278775825084 22.999729827382485 64 22.9766657606617 27.035459515412118 26.993533728611908 22.99434099531427 65 23.005382996086364 27.042158068097816 26.881680528605855 23.070778407209964 66 22.92122183930832 27.187483825983698 26.93433097107248 22.956963363635506 67 23.05788812209053 27.099708633984587 26.86846473123593 22.973938512688953 68 23.02527885252017 27.17110441418022 27.128071316454722 22.675545416844887 69 23.078342825911957 27.14722323765018 26.82968272967 22.94475120676786 70 22.901771472841972 26.791036516689342 27.183221463401928 23.12397054706675 71 22.99553686666621 26.890223716552136 27.042076367723645 23.07216304905801 72 22.813779553317794 27.133309050404 26.96977361213782 23.083137784140384 73 22.943186771085365 27.027533764154317 26.993322849798464 23.035956614961854 74 22.930071694213193 27.128829862552184 26.878184940146316 23.062913503088303 75 22.96765658863303 27.092758229403135 26.97205879768002 22.967526384283815 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 158.0 1 189.5 2 275.0 3 326.0 4 323.0 5 341.5 6 350.5 7 395.0 8 449.0 9 555.5 10 831.5 11 1045.5 12 1090.0 13 1210.5 14 1414.0 15 1744.5 16 1992.0 17 2241.0 18 2897.0 19 4125.0 20 4946.0 21 6216.0 22 9485.0 23 13429.5 24 15375.0 25 18337.0 26 24879.0 27 31930.5 28 35402.0 29 40191.5 30 49335.5 31 58412.5 32 63135.0 33 67722.0 34 78208.0 35 89760.0 36 95413.0 37 99641.5 38 108078.5 39 115951.5 40 119616.0 41 125038.5 42 134449.0 43 141813.5 44 145190.0 45 147440.5 46 151444.0 47 154404.0 48 155611.0 49 156160.5 50 154949.0 51 150346.5 52 147505.0 53 143786.0 54 135869.5 55 124973.0 56 118274.0 57 112228.5 58 97554.0 59 80225.0 60 71525.0 61 64485.0 62 50158.5 63 37279.5 64 31687.0 65 26393.5 66 17493.5 67 11893.0 68 9899.0 69 8232.5 70 5473.5 71 3656.0 72 2931.0 73 2485.5 74 1760.0 75 1132.5 76 785.0 77 633.0 78 375.0 79 213.0 80 157.0 81 109.5 82 46.5 83 25.5 84 20.0 85 11.0 86 1.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.004133923497025853 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 3.2550578716739005E-5 18 0.0 19 0.0 20 0.0 21 3.2550578716739005E-5 22 3.2550578716739005E-5 23 3.2550578716739005E-5 24 0.0 25 3.2550578716739005E-5 26 0.0 27 0.0 28 0.08863522584568032 29 0.0 30 0.0 31 3.2550578716739005E-5 32 3.2550578716739005E-5 33 0.052438982312666534 34 1.6275289358369502E-4 35 1.3020231486695602E-4 36 3.2550578716739005E-5 37 2.2785405101717306E-4 38 0.0010416185189356482 39 2.2785405101717306E-4 40 1.3020231486695602E-4 41 6.510115743347801E-4 42 0.0012369219912360821 43 1.6275289358369502E-4 44 5.533598381845631E-4 45 0.001009067940218909 46 2.9295520845065107E-4 47 1.9530347230043403E-4 48 3.2550578716739005E-4 49 1.3020231486695602E-4 50 3.9060694460086806E-4 51 8.137644679184751E-4 52 3.9060694460086806E-4 53 4.231575233176071E-4 54 0.0027342486122060765 55 0.0015949783571202114 56 4.8825868075108505E-4 57 5.208092594678241E-4 58 7.812138892017361E-4 59 7.812138892017361E-4 60 0.0027016980334893374 61 0.0026040462973391204 62 0.0020832370378712963 63 0.0010416185189356482 64 0.0015298771996867333 65 0.0019855853017210793 66 0.0027667991909228152 67 0.0019855853017210793 68 0.0028644509270730326 69 0.0030272038206567275 70 0.003971170603442159 71 0.0027667991909228152 72 8.137644679184751E-4 73 7.486633104849971E-4 74 0.0021808887740215133 75 0.0015624277784034722 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 3072142.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.90374686800293 #Duplication Level Percentage of deduplicated Percentage of total 1 81.67085096003805 56.274276410448564 2 10.849194608674102 14.951003180755649 3 3.4400436715012153 7.110956950679855 4 1.541015456729483 4.247269558006728 5 0.7626924814828531 2.6276184841111756 6 0.46038464433390497 1.9033336197059367 7 0.2810330990040061 1.355496346071171 8 0.1956690614680195 1.0785865185033696 9 0.13646530874759907 0.846267398118759 >10 0.627815150888115 7.371409515642507 >50 0.026404229335029665 1.2146015634245517 >100 0.0082882966881279 0.9674725641820916 >500 1.4303110941621714E-4 0.05170789034957812 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.2550578716739005E-5 2 0.0 0.0 0.0 0.0 3.2550578716739005E-5 3 0.0 0.0 0.0 0.0 3.2550578716739005E-5 4 0.0 0.0 0.0 0.0 3.2550578716739005E-5 5 0.0 0.0 0.0 0.0 3.2550578716739005E-5 6 0.0 0.0 0.0 0.0 3.2550578716739005E-5 7 0.0 0.0 0.0 6.510115743347801E-5 3.2550578716739005E-5 8 0.0 0.0 0.0 6.510115743347801E-5 3.2550578716739005E-5 9 0.0 0.0 0.0 6.510115743347801E-5 3.2550578716739005E-5 10 0.0 0.0 0.0 6.510115743347801E-5 3.2550578716739005E-5 11 0.0 0.0 0.0 6.510115743347801E-5 3.2550578716739005E-5 12 0.0 0.0 0.0 9.765173615021701E-5 3.2550578716739005E-5 13 0.0 0.0 0.0 9.765173615021701E-5 3.2550578716739005E-5 14 0.0 0.0 0.0 9.765173615021701E-5 3.2550578716739005E-5 15 0.0 0.0 0.0 9.765173615021701E-5 3.2550578716739005E-5 16 0.0 0.0 0.0 9.765173615021701E-5 3.2550578716739005E-5 17 0.0 0.0 0.0 9.765173615021701E-5 3.2550578716739005E-5 18 0.0 0.0 0.0 9.765173615021701E-5 3.2550578716739005E-5 19 0.0 0.0 0.0 1.3020231486695602E-4 3.2550578716739005E-5 20 0.0 0.0 0.0 1.3020231486695602E-4 3.2550578716739005E-5 21 0.0 0.0 0.0 1.3020231486695602E-4 3.2550578716739005E-5 22 0.0 0.0 0.0 1.3020231486695602E-4 3.2550578716739005E-5 23 0.0 0.0 0.0 1.3020231486695602E-4 3.2550578716739005E-5 24 0.0 0.0 0.0 1.6275289358369502E-4 3.2550578716739005E-5 25 0.0 0.0 0.0 2.2785405101717303E-4 3.2550578716739005E-5 26 0.0 0.0 0.0 2.2785405101717303E-4 3.2550578716739005E-5 27 0.0 0.0 0.0 2.6040462973391204E-4 3.2550578716739005E-5 28 0.0 0.0 0.0 4.231575233176071E-4 3.2550578716739005E-5 29 0.0 0.0 0.0 6.510115743347801E-4 3.2550578716739005E-5 30 0.0 0.0 0.0 0.0010416185189356482 3.2550578716739005E-5 31 0.0 0.0 0.0 0.0024087428250386864 3.2550578716739005E-5 32 0.0 0.0 0.0 0.0033201590291073784 3.2550578716739005E-5 33 0.0 0.0 0.0 0.004557081020343461 3.2550578716739005E-5 34 0.0 0.0 0.0 0.005924205326446499 3.2550578716739005E-5 35 0.0 0.0 0.0 0.00817019525790149 3.2550578716739005E-5 36 0.0 0.0 0.0 0.011067196763691261 3.2550578716739005E-5 37 0.0 0.0 0.0 0.01503836736713342 3.2550578716739005E-5 38 0.0 0.0 0.0 0.02057196574897905 3.2550578716739005E-5 39 0.0 0.0 0.0 0.026300867603125114 3.2550578716739005E-5 40 0.0 0.0 0.0 0.03245292698058879 3.2550578716739005E-5 41 0.0 0.0 0.0 0.037368064366816375 3.2550578716739005E-5 42 0.0 0.0 0.0 0.044073483582464615 3.2550578716739005E-5 43 3.2550578716739005E-5 0.0 0.0 0.05097420627041328 3.2550578716739005E-5 44 3.2550578716739005E-5 0.0 0.0 0.05803768185194565 3.2550578716739005E-5 45 3.2550578716739005E-5 0.0 0.0 0.06497095511861105 3.2550578716739005E-5 46 3.2550578716739005E-5 0.0 0.0 0.07385726310828081 3.2550578716739005E-5 47 3.2550578716739005E-5 0.0 0.0 0.08199490778746556 3.2550578716739005E-5 48 3.2550578716739005E-5 0.0 0.0 0.09325740802345725 3.2550578716739005E-5 49 3.2550578716739005E-5 0.0 0.0 0.10328298626821286 3.2550578716739005E-5 50 6.510115743347801E-5 0.0 0.0 0.11412232898088695 3.2550578716739005E-5 51 6.510115743347801E-5 0.0 0.0 0.1250267728509945 3.2550578716739005E-5 52 6.510115743347801E-5 0.0 0.0 0.13739599276335535 3.2550578716739005E-5 53 6.510115743347801E-5 0.0 0.0 0.1474541215868277 3.2550578716739005E-5 54 6.510115743347801E-5 0.0 0.0 0.16128811754144176 3.2550578716739005E-5 55 6.510115743347801E-5 0.0 0.0 0.17489425944503867 3.2550578716739005E-5 56 6.510115743347801E-5 0.0 0.0 0.1884353001912021 3.2550578716739005E-5 57 6.510115743347801E-5 0.0 0.0 0.20340856640090205 3.2550578716739005E-5 58 6.510115743347801E-5 0.0 3.2550578716739005E-5 0.21870733839776937 3.2550578716739005E-5 59 6.510115743347801E-5 0.0 6.510115743347801E-5 0.23446181849667105 3.2550578716739005E-5 60 6.510115743347801E-5 0.0 6.510115743347801E-5 0.2534713564672466 3.2550578716739005E-5 61 6.510115743347801E-5 0.0 6.510115743347801E-5 0.2717973322847707 3.2550578716739005E-5 62 6.510115743347801E-5 0.0 6.510115743347801E-5 0.2914904324083978 3.2550578716739005E-5 63 6.510115743347801E-5 0.0 6.510115743347801E-5 0.3125181062594112 3.2550578716739005E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3205 0.0 36.487667 1 ATCAACG 3970 0.0 27.977669 3 TCAACGC 3940 0.0 27.92805 4 CAACGCA 3950 0.0 27.77002 5 AACGCAG 4185 0.0 26.12823 6 TATCAAC 4435 0.0 25.744268 2 GGTATCA 1830 0.0 23.374645 1 ACGCAGA 4805 0.0 22.254328 7 GTACATG 7290 0.0 20.631622 1 CGCAGAG 5215 0.0 20.173985 8 TACATGG 7400 0.0 19.43795 2 AGAGTAC 5370 0.0 18.820862 11 GTATAGG 965 0.0 18.58876 1 GTATAGC 1100 0.0 18.502642 1 ACATGGG 7300 0.0 18.2394 3 CCTATAC 785 0.0 16.697832 3 CAGAGTA 6135 0.0 16.474007 10 CATGGGG 5350 0.0 16.441164 4 TATACTG 1325 0.0 16.140684 5 GCAGAGT 6350 0.0 16.133509 9 >>END_MODULE