FastQCFastQC Report
Mon 6 Feb 2017
SRR3203973_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203973_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3072142
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56100.040.027281
GGTATCA25750.035.9704251
ATCAACG74550.029.00523
TATCAAC76000.028.944822
TCAACGC74800.028.849894
CAACGCA76450.028.3929335
AACGCAG79350.027.3552576
ACGCAGA89150.024.1082637
CGCAGAG91850.023.2881588
AGAGTAC94550.021.76532611
GAGTACT70200.021.36933712
GTACATG71350.021.3166791
TCGATCG1851.1184966E-820.60637541
CAGAGTA103500.020.29963110
GCGATAC3050.020.21578414
TACATGG71550.020.1943242
AGTACTT74100.020.15482913
GCAGAGT105600.020.1546619
ACATGGG71150.019.5886763
TATACCG1601.5118458E-619.1978745
Produced by FastQC (version 0.11.5)