##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3203973_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3072142
Sequences flagged as poor quality	0
Sequence length	75
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.76115850113699	35.0	33.0	35.0	27.0	35.0
2	32.81978697599265	35.0	33.0	35.0	27.0	35.0
3	32.74730432382357	35.0	32.0	35.0	30.0	35.0
4	32.8140883461767	35.0	33.0	35.0	30.0	35.0
5	32.89842819765492	35.0	33.0	35.0	30.0	35.0
6	36.42896910364169	39.0	36.0	40.0	27.0	40.0
7	36.26615338744107	39.0	36.0	40.0	27.0	40.0
8	36.08997761171196	39.0	36.0	40.0	27.0	40.0
9	36.07023633673183	39.0	36.0	40.0	27.0	40.0
10	36.03134816033894	39.0	36.0	40.0	27.0	40.0
11	36.112925118695685	39.0	36.0	40.0	27.0	40.0
12	36.10769717024799	39.0	36.0	40.0	27.0	40.0
13	36.09388237913482	39.0	36.0	40.0	27.0	40.0
14	36.031803217429406	39.0	36.0	40.0	27.0	40.0
15	36.017638182089236	39.0	36.0	40.0	27.0	40.0
16	35.998073656751544	39.0	36.0	40.0	27.0	40.0
17	35.73283624259556	39.0	36.0	40.0	27.0	40.0
18	35.68953518424604	39.0	35.0	40.0	27.0	40.0
19	35.895593367754486	39.0	36.0	40.0	27.0	40.0
20	35.881753187189915	39.0	36.0	40.0	27.0	40.0
21	35.67378070414714	39.0	35.0	40.0	27.0	40.0
22	35.67133094759292	39.0	35.0	40.0	27.0	40.0
23	35.476205201452274	39.0	35.0	40.0	27.0	40.0
24	35.52226622337118	39.0	35.0	40.0	27.0	40.0
25	35.29246141617152	39.0	35.0	40.0	16.0	40.0
26	35.336583725622056	39.0	35.0	40.0	27.0	40.0
27	35.42647833335829	39.0	35.0	40.0	27.0	40.0
28	35.41244708089665	39.0	35.0	40.0	27.0	40.0
29	35.3911515157828	39.0	35.0	40.0	27.0	40.0
30	35.304545493014324	39.0	35.0	40.0	16.0	40.0
31	35.35010914209044	39.0	35.0	40.0	27.0	40.0
32	35.12991912483212	39.0	35.0	40.0	16.0	40.0
33	35.33627416961846	39.0	35.0	40.0	27.0	40.0
34	35.358466177670174	39.0	35.0	40.0	27.0	40.0
35	35.4093358965829	39.0	35.0	40.0	27.0	40.0
36	35.39469562279348	39.0	35.0	40.0	27.0	40.0
37	35.19648863887151	39.0	35.0	40.0	16.0	40.0
38	35.246486327780424	39.0	35.0	40.0	16.0	40.0
39	35.2628120705358	39.0	35.0	40.0	27.0	40.0
40	35.31618167389398	39.0	35.0	40.0	27.0	40.0
41	35.43704327469238	39.0	35.0	40.0	27.0	40.0
42	35.16702385501712	39.0	35.0	40.0	16.0	40.0
43	35.12413749104045	39.0	35.0	40.0	16.0	40.0
44	35.166652778419746	39.0	35.0	40.0	16.0	40.0
45	34.99546049629216	39.0	34.0	40.0	16.0	40.0
46	34.91843801490947	39.0	34.0	40.0	16.0	40.0
47	35.00077437826767	39.0	34.0	40.0	16.0	40.0
48	34.88480773349669	39.0	34.0	40.0	16.0	40.0
49	34.893512734762915	39.0	34.0	40.0	16.0	40.0
50	34.81484742567238	39.0	34.0	40.0	16.0	40.0
51	34.49457381852792	39.0	34.0	40.0	16.0	40.0
52	34.78404448752694	39.0	34.0	40.0	16.0	40.0
53	34.79036222935007	39.0	34.0	40.0	16.0	40.0
54	34.729561328870865	39.0	34.0	40.0	16.0	40.0
55	34.65419111486383	39.0	34.0	40.0	16.0	40.0
56	34.67810309549493	39.0	34.0	40.0	16.0	40.0
57	34.61319170793538	39.0	34.0	40.0	16.0	40.0
58	34.669129877460094	39.0	34.0	40.0	16.0	40.0
59	34.62129940608214	39.0	34.0	40.0	16.0	40.0
60	34.31982831522762	39.0	34.0	40.0	16.0	40.0
61	34.55824600555573	39.0	34.0	40.0	16.0	40.0
62	34.29849043436143	39.0	34.0	40.0	16.0	40.0
63	34.45649354749878	39.0	34.0	40.0	16.0	40.0
64	34.43869521656225	39.0	34.0	40.0	16.0	40.0
65	34.39257853315374	39.0	34.0	40.0	16.0	40.0
66	34.40509716022241	39.0	34.0	40.0	16.0	40.0
67	34.385392341890444	39.0	34.0	40.0	16.0	40.0
68	34.316716805408085	39.0	34.0	40.0	16.0	40.0
69	34.205894128591716	39.0	34.0	40.0	16.0	40.0
70	34.125966508058546	39.0	34.0	40.0	16.0	40.0
71	34.09320467608594	39.0	34.0	40.0	16.0	40.0
72	34.09748768123348	39.0	34.0	40.0	16.0	40.0
73	33.94040737700276	39.0	34.0	40.0	16.0	40.0
74	33.87216020613631	39.0	34.0	40.0	16.0	40.0
75	33.80400417689026	39.0	34.0	40.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	713.0
3	1386.0
4	1276.0
5	1266.0
6	1572.0
7	2227.0
8	3563.0
9	6014.0
10	5699.0
11	4126.0
12	4283.0
13	4840.0
14	2769.0
15	3198.0
16	3752.0
17	4494.0
18	5252.0
19	7480.0
20	13621.0
21	22948.0
22	32330.0
23	40824.0
24	49975.0
25	57308.0
26	62051.0
27	67770.0
28	73680.0
29	80438.0
30	87714.0
31	94638.0
32	102984.0
33	113398.0
34	126364.0
35	144587.0
36	175665.0
37	237619.0
38	406973.0
39	1017345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.191132466346126	17.82156187358632	13.222852678741589	24.76445298132597
2	15.043933516093983	19.542065438381428	40.82366635396411	24.590334691560482
3	18.99821776716478	25.1953927018005	28.490846396148058	27.31554313488666
4	12.04893161565064	16.724739470280937	38.0108129725709	33.21551594149753
5	13.693948532990024	37.97995810635517	34.38765130295094	13.938442057703856
6	31.97888852087748	37.76506998567361	17.062539467594966	13.19350202585394
7	28.111070386720407	31.788764972452444	21.537350812560096	18.562813828267053
8	25.74758850556526	36.30854887758766	19.27011049135593	18.67375212549115
9	26.05317888066083	15.881073265084837	18.826181824337105	39.23956602991723
10	13.488526065081082	29.766869120745593	34.22611035901068	22.518494455162642
11	35.484905241516785	23.063637363832083	23.21688628656507	18.23457110808606
12	23.364283982103416	24.66046785662553	30.950149653575167	21.025098507695887
13	28.22849899791706	21.909989287461578	25.858524833656183	24.002986880965178
14	21.671681202472993	20.75148702714685	27.777370885875285	29.799460884504875
15	23.31911566377463	29.16976860995072	24.0352291781345	23.475886548140153
16	23.236382824893038	28.47569477110502	25.331593265560105	22.956329138441838
17	21.996942123133287	27.928141847963456	26.78557469062937	23.289341338273882
18	22.075258120948103	27.435157371699066	27.920413320200648	22.569171187152186
19	22.966429755133536	27.011333335503846	28.179471067599554	21.842765841763065
20	22.96934644491362	26.516928556429338	28.349858460697003	22.163866537960043
21	23.24381117037438	26.190214830975545	28.183375052843623	22.382598945806453
22	22.63502829288692	26.35077299042093	28.255870937389737	22.758327779302416
23	22.78460818339499	26.286486257118636	27.918875899111967	23.010029660374403
24	22.382028055676027	26.3989074853708	28.260981447116396	22.958083011836777
25	22.802515115832925	26.283688130928912	28.155603773335276	22.758192979902887
26	22.548307228148797	26.606466357880223	28.183665326457618	22.66156108751336
27	22.645015915128948	26.755353800757497	27.9292198088082	22.67041047530535
28	22.58991574069869	26.68473677830776	27.887381557834868	22.83796592315868
29	22.837851556198892	26.656063897834954	27.92865898190422	22.577425564061937
30	22.71581567381064	26.524404867897722	28.05713820127486	22.702641257016772
31	22.801881133384825	26.373734443141917	27.976108795742682	22.848275627730576
32	22.522420580619357	26.626840750721037	28.076218132249846	22.77452053640976
33	22.632993908156156	26.43476541425062	28.117322993436733	22.81491768415649
34	22.826721225027754	26.65192710943713	28.065656918781272	22.455694746753846
35	22.88511329239746	26.500472902444884	28.08161691798287	22.53279688717479
36	22.77375794352774	26.71829014723177	27.879867762901434	22.62808414633906
37	22.722332922619874	26.432568765997928	27.95929134495391	22.885806966428284
38	22.7145397509127	26.514285676741046	27.94710763132504	22.82406694102122
39	22.89774085095378	26.771063512173082	27.74906782684986	22.582127810023277
40	22.96818255732018	26.424689192318873	28.074971625299554	22.53215662506139
41	22.714186263867113	26.59843213684975	27.83840758365677	22.848974015626364
42	22.905429843294662	26.503447136299773	27.878031129511605	22.713091890893956
43	22.706736755236566	26.394285240465432	27.952301841873346	22.946676162424655
44	22.687044449735403	26.819438652893226	27.744285438045164	22.74923145932621
45	22.841647895641813	26.723003694973897	27.56482536414046	22.870523045243832
46	22.933099543875375	26.595358268843068	27.40693607330397	23.064606113977586
47	22.793630074743923	26.700357289149423	27.43613513617688	23.06987749992977
48	22.636882223668152	26.875409934593385	27.51053090939498	22.97717693234348
49	22.974373564075204	26.631038045456112	27.41725264659372	22.97733574387496
50	23.00232254956373	26.864981258473563	27.319432757551542	22.81326343441117
51	22.763555718124763	27.007501800069534	27.103955869050502	23.1249866127552
52	22.951166660242745	26.999118725163108	27.14522481654262	22.90448979805153
53	22.817718646231356	27.019027939916995	27.284420894607802	22.878832519243847
54	22.69837811565084	26.965064468049178	27.416061531848257	22.920495884451725
55	22.785301541381354	26.775498553676336	27.358119690853023	23.08108021408929
56	22.60747370589995	27.164846454043957	27.3734024244835	22.85427741557259
57	22.900845111135272	26.978354972718293	27.012566967293854	23.10823294885258
58	22.83511352585915	26.876600793173523	27.374906618696905	22.913379062270426
59	22.93891677346501	27.078742794977455	27.152440975537345	22.829899456020186
60	22.845279553524794	27.166824324057643	27.238033277769517	22.749862844648046
61	23.01297901456884	26.884873744969134	27.373246928095895	22.728900312366132
62	22.766819532577916	27.24124872388986	27.273390824706734	22.718540918825482
63	22.795695914595363	26.805092872595505	27.522917861576836	22.876293351232302
64	22.759814835138993	26.906360567149047	27.405333615549036	22.928490982162923
65	22.827027269415463	26.86488951063783	27.309053047920894	22.999030172025815
66	22.74099886124583	27.054559281302232	27.285793439499862	22.918648417952078
67	22.92150589758123	26.884935717650553	27.343251240443756	22.850307144324464
68	22.82796872975537	27.04487463710226	27.49480598033337	22.632350652809002
69	22.972046350612835	26.96645620875874	27.215647431661072	22.845850008967357
70	22.704646162548908	26.725844721115017	27.445121280529644	23.124387835806434
71	22.85507490803766	26.73640979290648	27.365576098713078	23.04293920034278
72	22.683261641270793	26.97381796491012	27.279334516069575	23.06358587774951
73	22.922697111930166	26.833176681478864	27.254687916623666	22.989438289967303
74	22.855335828128542	26.90994753044844	27.17889915792448	23.05581748349854
75	22.89969766043508	26.901837033697735	27.262872558108022	22.935592747759163
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	107.0
1	107.0
2	224.5
3	389.5
4	437.0
5	369.0
6	363.5
7	633.5
8	841.0
9	1546.0
10	3036.5
11	3878.5
12	3935.0
13	3858.0
14	3934.5
15	4391.0
16	4694.0
17	5069.5
18	5982.0
19	7289.0
20	8059.0
21	9267.5
22	12341.5
23	16105.0
24	18003.0
25	20705.5
26	26915.0
27	33477.0
28	36532.0
29	40852.5
30	49506.0
31	58215.0
32	62591.0
33	66934.5
34	76797.0
35	87808.0
36	93300.0
37	97179.0
38	105361.0
39	113888.5
40	118113.0
41	123450.5
42	132507.5
43	139805.0
44	143383.0
45	145641.5
46	149506.0
47	151880.5
48	152649.0
49	153581.5
50	152998.5
51	148924.5
52	146366.0
53	143018.0
54	135223.5
55	123992.5
56	117208.0
57	111207.5
58	96947.5
59	79743.5
60	70799.0
61	63685.5
62	49553.5
63	36814.5
64	31094.0
65	25673.5
66	16822.0
67	11496.0
68	9601.0
69	8013.5
70	5375.5
71	3503.0
72	2681.0
73	2334.5
74	1656.5
75	1024.0
76	723.0
77	586.5
78	349.0
79	187.5
80	127.0
81	94.5
82	42.0
83	13.5
84	5.0
85	3.0
86	2.0
87	2.0
88	1.0
89	1.0
90	1.5
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.08414324598277033
2	0.0
3	0.04150198786384223
4	0.05113695916399698
5	0.0025063945611889034
6	0.05159266726603132
7	0.0
8	0.016242738779652766
9	0.004329226969326288
10	1.6275289358369502E-4
11	5.208092594678241E-4
12	0.0015298771996867333
13	0.0013345737273862991
14	0.0023761922463219473
15	0.0046547327564936776
16	0.007519183683566711
17	0.06633807942471409
18	0.36056276044531793
19	0.021873988897648612
20	0.018130672345223628
21	0.3653476955166786
22	0.3678866406565842
23	1.0435390030799356
24	0.7019532300264766
25	1.5891843541086317
26	1.0438645088671032
27	0.9173729599738554
28	0.37338768845971315
29	0.9206280178455293
30	0.9232646147215852
31	0.37218331704719376
32	1.0441574640755538
33	0.5717834657382374
34	0.3725088228343612
35	0.36642186461433096
36	0.9676636040912172
37	1.038493663378841
38	0.9162662402974862
39	0.5680726997645291
40	0.9132715870555462
41	0.35913053498178144
42	0.9120997662217436
43	0.6820973770092659
44	1.0192888219359653
45	0.347835484167073
46	0.3482260911116739
47	0.35011402467724473
48	0.34757507953733907
49	0.0026040462973391204
50	0.0028319003483562935
51	1.2218510732902321
52	0.34724957375017174
53	0.34646835986096997
54	0.34838884400525755
55	0.5584051778856576
56	0.0021157876165880355
57	0.0039060694460086804
58	0.35053718220056235
59	0.004980238543661067
60	1.03491309972
61	0.003450361343974334
62	0.5520252644571768
63	0.0027342486122060765
64	0.34679386564813736
65	0.5520903656146102
66	0.011848410652892999
67	0.014940715630983202
68	0.011067196763691261
69	0.3593258384540819
70	0.5665753731435591
71	0.02525924908418947
72	0.5742898602994263
73	0.018716582762124928
74	0.9267800772229929
75	0.06783540604568408
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	3072142.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.06565813628075
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.23183934972035	52.34503611987341
2	12.153465719585437	16.058534228022776
3	4.026954913441664	7.981302799249589
4	1.747479064496228	4.617934179012619
5	0.8655185625233781	2.859052673113731
6	0.5154060921567863	2.0430385611471995
7	0.3349282181086001	1.5489077210429512
8	0.21578493607249793	1.1404779054019876
9	0.16498336582178055	0.9809761180099229
>10	0.7037855023547493	7.882127512997863
>50	0.030155895162218192	1.3235208391292024
>100	0.009399778572112378	1.060779466898693
>500	2.4883498687792587E-4	0.10659337543261105
>1k	4.976699723463892E-5	0.05171850066740747
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.2550578716739005E-5	0.0
10	0.0	0.0	0.0	3.2550578716739005E-5	0.0
11	0.0	0.0	0.0	3.2550578716739005E-5	0.0
12	0.0	0.0	0.0	3.2550578716739005E-5	0.0
13	3.2550578716739005E-5	0.0	0.0	3.2550578716739005E-5	0.0
14	3.2550578716739005E-5	0.0	0.0	3.2550578716739005E-5	0.0
15	3.2550578716739005E-5	0.0	0.0	3.2550578716739005E-5	0.0
16	3.2550578716739005E-5	0.0	0.0	3.2550578716739005E-5	0.0
17	3.2550578716739005E-5	0.0	0.0	3.2550578716739005E-5	0.0
18	3.2550578716739005E-5	0.0	0.0	3.2550578716739005E-5	0.0
19	3.2550578716739005E-5	0.0	0.0	3.2550578716739005E-5	0.0
20	3.2550578716739005E-5	0.0	0.0	3.2550578716739005E-5	0.0
21	3.2550578716739005E-5	0.0	0.0	3.2550578716739005E-5	0.0
22	3.2550578716739005E-5	0.0	0.0	3.2550578716739005E-5	0.0
23	3.2550578716739005E-5	0.0	0.0	3.2550578716739005E-5	0.0
24	6.510115743347801E-5	0.0	0.0	6.510115743347801E-5	0.0
25	6.510115743347801E-5	0.0	0.0	6.510115743347801E-5	0.0
26	6.510115743347801E-5	0.0	0.0	6.510115743347801E-5	0.0
27	6.510115743347801E-5	0.0	0.0	1.3020231486695602E-4	0.0
28	6.510115743347801E-5	0.0	0.0	2.6040462973391204E-4	0.0
29	6.510115743347801E-5	0.0	0.0	4.5570810203434607E-4	0.0
30	6.510115743347801E-5	0.0	0.0	8.788656253519531E-4	0.0
31	6.510115743347801E-5	0.0	0.0	0.002148338195304774	0.0
32	6.510115743347801E-5	0.0	0.0	0.0030597543993734666	0.0
33	6.510115743347801E-5	0.0	0.0	0.004329226969326288	0.0
34	6.510115743347801E-5	0.0	0.0	0.0055335983818456305	0.0
35	6.510115743347801E-5	0.0	0.0	0.007747037734583883	0.0
36	6.510115743347801E-5	0.0	0.0	0.0105138369255067	0.0
37	6.510115743347801E-5	0.0	0.0	0.014224602899214945	0.0
38	6.510115743347801E-5	0.0	0.0	0.019432695493893187	0.0
39	6.510115743347801E-5	0.0	0.0	0.024998844454455556	0.0
40	6.510115743347801E-5	0.0	0.0	0.030923049780902056	0.0
41	6.510115743347801E-5	0.0	0.0	0.03567543427354595	0.0
42	6.510115743347801E-5	0.0	0.0	0.04215299943817701	0.0
43	6.510115743347801E-5	0.0	0.0	0.04872821633895829	0.0
44	6.510115743347801E-5	0.0	0.0	0.05517323092487261	0.0
45	6.510115743347801E-5	0.0	0.0	0.061618245510786936	0.0
46	6.510115743347801E-5	0.0	0.0	0.06991864308355539	0.0
47	6.510115743347801E-5	0.0	0.0	0.0778609842904397	0.0
48	6.510115743347801E-5	0.0	0.0	0.08827716947979618	0.0
49	6.510115743347801E-5	0.0	0.0	0.0978144890438007	0.0
50	6.510115743347801E-5	0.0	0.0	0.10800282018214002	0.0
51	6.510115743347801E-5	0.0	0.0	0.11793074669074541	0.0
52	6.510115743347801E-5	0.0	0.0	0.1293559998203208	0.0
53	6.510115743347801E-5	0.0	0.0	0.13850271243972448	0.0
54	6.510115743347801E-5	0.0	0.0	0.15152294392642007	0.0
55	6.510115743347801E-5	0.0	0.0	0.16451062483439893	0.0
56	6.510115743347801E-5	0.0	0.0	0.17704259764034344	0.0
57	6.510115743347801E-5	0.0	0.0	0.1908765935949575	0.0
58	6.510115743347801E-5	0.0	0.0	0.20542670228133986	0.0
59	6.510115743347801E-5	0.0	0.0	0.22026976617617286	0.0
60	6.510115743347801E-5	0.0	0.0	0.2389212477808643	0.0
61	6.510115743347801E-5	0.0	0.0	0.25649856028790335	0.0
62	6.510115743347801E-5	0.0	0.0	0.2744664797395433	0.0
63	6.510115743347801E-5	0.0	0.0	0.29455018680777123	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5610	0.0	40.02728	1
GGTATCA	2575	0.0	35.970425	1
ATCAACG	7455	0.0	29.0052	3
TATCAAC	7600	0.0	28.94482	2
TCAACGC	7480	0.0	28.84989	4
CAACGCA	7645	0.0	28.392933	5
AACGCAG	7935	0.0	27.355257	6
ACGCAGA	8915	0.0	24.108263	7
CGCAGAG	9185	0.0	23.288158	8
AGAGTAC	9455	0.0	21.765326	11
GAGTACT	7020	0.0	21.369337	12
GTACATG	7135	0.0	21.316679	1
TCGATCG	185	1.1184966E-8	20.606375	41
CAGAGTA	10350	0.0	20.299631	10
GCGATAC	305	0.0	20.215784	14
TACATGG	7155	0.0	20.194324	2
AGTACTT	7410	0.0	20.154829	13
GCAGAGT	10560	0.0	20.154661	9
ACATGGG	7115	0.0	19.588676	3
TATACCG	160	1.5118458E-6	19.197874	5
>>END_MODULE