FastQCFastQC Report
Mon 6 Feb 2017
SRR3203972_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203972_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3595867
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36600.036.475921
ATCAACG44300.028.8104633
TCAACGC44500.028.6809774
CAACGCA45850.028.06225
GGTATCA17350.027.2393971
TATCAAC49800.025.9749162
AACGCAG48700.025.853316
GTACATG81950.022.9837061
ACGCAGA54800.022.5348537
TACATGG82350.021.6979332
CGCAGAG56200.021.6665928
ACATGGG82500.020.7804363
AGAGTAC57950.019.22654711
CAGAGTA64450.017.71565810
CATGGGG53850.017.3594464
GCAGAGT68850.017.0845159
GAGTACT29200.015.71162112
GTATATA16950.015.6710251
CGTGCCG4000.015.5226110
CATGGGT23900.015.154574
Produced by FastQC (version 0.11.5)