FastQCFastQC Report
Mon 6 Feb 2017
SRR3203972_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203972_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3595867
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55650.041.1520231
GGTATCA23150.037.0599021
TATCAAC77350.029.1023712
ATCAACG75750.029.086163
TCAACGC75200.029.0150344
CAACGCA75700.028.6337935
AACGCAG79000.027.4376986
ACGCAGA92100.023.4501577
AATCTCG13750.023.20135936
CTCGTAT16350.022.89000539
CGCAGAG94900.022.722638
TATGCCG17300.021.90801443
GTACATG79900.021.6036011
TCGTATG17850.021.35474240
AGAGTAC97150.021.01132211
CGTGCCG3100.020.90928610
TCTCGTA15850.020.7704838
GAGTACT70150.020.65249312
TACATGG82750.020.0205972
CAGAGTA104650.019.98336210
Produced by FastQC (version 0.11.5)