FastQCFastQC Report
Mon 6 Feb 2017
SRR3203971_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203971_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4387287
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42050.036.669341
TCAACGC49950.029.9019224
ATCAACG50600.029.5178053
CAACGCA51300.029.1150285
GGTATCA20800.028.0275361
AACGCAG54600.027.4816846
TATCAAC56000.026.7946432
ACGCAGA65000.022.5539327
CGCAGAG68000.021.203828
GTACATG91250.020.6027491
AGAGTAC68950.020.01116811
TACATGG93900.019.6532732
CAGAGTA77100.018.25376710
ACATGGG96500.018.2301243
GCAGAGT83250.016.988169
CATGGGG76600.016.1663654
GAGTACT33050.015.44674512
TCTATAC9750.014.8590613
GTATAAT15100.014.8490271
GTATATA19350.014.796491
Produced by FastQC (version 0.11.5)