FastQCFastQC Report
Mon 6 Feb 2017
SRR3203971_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203971_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4387287
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61300.040.7779161
GGTATCA26900.036.052921
TCAACGC82250.029.2837564
ATCAACG83700.028.869173
TATCAAC85750.028.7740422
CAACGCA83750.028.7454935
AACGCAG87850.027.6371386
ACGCAGA102900.023.5831387
CGCAGAG106400.022.6154388
AGAGTAC107700.021.43496911
GAGTACT77900.021.13900612
GTACATG87600.021.050581
CGTACTA11450.020.89344829
CAGAGTA117200.020.15060810
TACATGG88300.020.0973782
GCAGAGT121350.019.7149059
AGTACTT84900.019.67994713
GTACTTT86950.018.94096614
ACATGGG91150.018.8337923
ACCGTAC13150.017.6650827
Produced by FastQC (version 0.11.5)