FastQCFastQC Report
Mon 6 Feb 2017
SRR3203970_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203970_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4342226
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41950.037.7426721
TCAACGC48550.029.9823114
ATCAACG49150.029.475943
CAACGCA49750.029.2591195
TATCAAC54750.027.217082
AACGCAG54750.026.3980486
GGTATCA20350.023.900481
GTACATG100100.023.2606351
TTACGCG902.129907E-422.99644343
TACATGG101100.022.3135412
ACGCAGA63700.021.9851027
ACATGGG105550.020.5231173
CGCAGAG67600.020.4105748
AGAGTAC69100.018.86929511
CATGGGG75350.017.8077164
CAGAGTA78800.016.89674210
GTATAGA13700.016.6178671
GCAGAGT84350.016.1530279
GTATAGC13150.016.0013261
TACGCGA1756.371538E-515.76898944
Produced by FastQC (version 0.11.5)