FastQCFastQC Report
Mon 6 Feb 2017
SRR3203970_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203970_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4342226
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68950.042.2109071
GGTATCA28350.032.7744371
ATCAACG90850.030.8519083
TCAACGC90000.030.7908764
TATCAAC93250.030.3866652
CAACGCA91250.030.2055515
AACGCAG96500.028.6329466
ACGCAGA108400.025.1298927
CGCAGAG114350.023.5539658
GGCGAAT13250.023.30187832
GTACATG99500.023.2769431
AGAGTAC117550.022.22257211
TACATGG101650.021.7967192
CTCGTAT15400.021.6085939
TATGCCG16700.021.4576143
GCGAATC13200.021.28740933
CAGAGTA125600.021.11290710
GAGTACT81500.020.7931512
ACATGGG102050.020.6747043
GCAGAGT132050.020.2114039
Produced by FastQC (version 0.11.5)