FastQCFastQC Report
Mon 6 Feb 2017
SRR3203969_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203969_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3379096
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34950.032.8655471
TCAACGC38450.027.4512734
ATCAACG39450.026.842863
CAACGCA40500.026.2321035
AACGCAG43100.024.809726
TATCAAC45200.024.5728112
GGTATCA18500.021.815141
GTACATG74550.021.4691141
TACATGG75400.020.815052
ACGCAGA50050.020.6754327
ACATGGG76850.020.1081243
CGCAGAG50600.019.9735168
AGAGTAC50450.018.2552411
CATGGGG56550.017.688984
CGTATAC1959.008736E-615.9200823
GTATTAG8500.015.826671
CAGAGTA60150.015.77010110
AATACCG2554.608919E-714.8795545
ACCGTGC9050.014.8646068
GTATATA16050.014.82920551
Produced by FastQC (version 0.11.5)