FastQCFastQC Report
Mon 6 Feb 2017
SRR3203969_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203969_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3379096
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53000.038.758771
GGTATCA23550.030.8204921
TCAACGC69050.028.9736964
ATCAACG70200.028.5117283
TATCAAC71500.028.2317582
CAACGCA70150.028.1626725
AACGCAG72650.027.241816
ACGCAGA85050.023.0578277
CGCAGAG86700.022.54028
GTACATG75100.021.7000941
GAGTACT59000.021.49100712
AGAGTAC85900.021.484711
TACATGG78200.020.650342
TATGCCG13900.020.55644443
GCGAATC11000.020.4941633
GGCGAAT11550.020.11880932
AGTACTT64600.020.00083513
ACATGGG77650.019.6516133
CAGAGTA96150.019.64537210
TCGTATG15000.019.61564440
Produced by FastQC (version 0.11.5)