FastQCFastQC Report
Mon 6 Feb 2017
SRR3203968_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203968_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4163361
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA40250.034.8809171
TCAACGC45250.029.1198084
CAACGCA44950.029.083945
ATCAACG46050.028.539023
TATCAAC49150.027.86192
AACGCAG48000.027.020316
GGTATCA18950.024.3924261
ACGCAGA55200.022.871037
GTACATG89100.021.9902421
CGCAGAG57150.021.4870858
TACATGG89150.021.2419662
ACATGGG90350.019.5472433
CGCCCAA6800.018.76970350
AGAGTAC59300.018.67213811
CATGGGG68500.016.818984
GTACGCT6000.016.67330760
TGTACGC6250.016.55792259
CGACTCG2301.4270154E-716.49832565
GCGATAC5250.016.42572214
CGTGCCG5350.016.11869810
Produced by FastQC (version 0.11.5)