FastQCFastQC Report
Mon 6 Feb 2017
SRR3203968_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203968_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4163361
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61700.038.719991
GGTATCA27200.034.8061
CAACGCA81450.027.8260085
TATCAAC84200.027.4234312
TCAACGC83000.027.399814
ATCAACG84050.027.3511123
AACGCAG85850.026.4396256
CGTGCCG4550.023.239510
ACGCAGA98050.023.0351587
CGCAGAG100700.022.2256878
GTACATG84850.020.9054131
AGAGTAC104050.020.50051911
GAGTACT74050.020.3500512
TACATGG86000.019.898782
CTCGTAT13800.019.8410639
AATCTCG12650.019.45932636
AGTACTT78800.018.95088813
TCGTATG16000.018.84586740
CAGAGTA116250.018.71992910
TATGCCG15100.018.71527343
Produced by FastQC (version 0.11.5)