FastQCFastQC Report
Mon 6 Feb 2017
SRR3203967_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203967_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148775
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32900.036.170181
ATCAACG39250.029.1760143
TCAACGC38900.028.9951734
CAACGCA39800.028.5994995
TATCAAC42650.027.416262
AACGCAG41150.027.4097756
GGTATCA16300.024.1237471
ACGCAGA46850.023.7068467
CGCAGAG50050.021.9843758
GTACATG67450.020.4041481
AGAGTAC51400.020.19904711
TACATGG67200.019.8127862
ACATGGG70850.017.8670863
GAGTACT25500.017.85505912
CAGAGTA59250.017.58110210
TCGATCG2451.5927071E-816.89520341
GCAGAGT63200.016.8643259
CGATCGT2753.741661E-916.3064342
GTCTAGG10700.016.1180971
CATGGGG52650.016.116264
Produced by FastQC (version 0.11.5)