##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3203967_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3148775
Sequences flagged as poor quality	0
Sequence length	75
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12752832450715	34.0	31.0	35.0	15.0	35.0
2	31.435616390501067	35.0	31.0	35.0	15.0	35.0
3	31.541361640638026	35.0	31.0	35.0	15.0	35.0
4	31.730189359354036	35.0	31.0	35.0	27.0	35.0
5	31.85947868615573	35.0	31.0	35.0	27.0	35.0
6	35.41498201681606	39.0	35.0	40.0	16.0	40.0
7	35.13348619701313	39.0	34.0	40.0	16.0	40.0
8	35.20563552492636	39.0	34.0	40.0	16.0	40.0
9	35.16248001206819	39.0	34.0	40.0	16.0	40.0
10	35.267747298552614	39.0	34.0	40.0	16.0	40.0
11	35.342097799938074	39.0	35.0	40.0	16.0	40.0
12	34.91717413914935	39.0	34.0	40.0	16.0	40.0
13	34.97749950377528	39.0	34.0	40.0	16.0	40.0
14	34.95809290914721	39.0	34.0	40.0	16.0	40.0
15	35.01127676636152	39.0	34.0	40.0	16.0	40.0
16	35.158654397344996	39.0	34.0	40.0	16.0	40.0
17	35.190448666544924	39.0	34.0	40.0	16.0	40.0
18	35.25035291502251	39.0	34.0	40.0	16.0	40.0
19	35.28459099173488	39.0	34.0	40.0	16.0	40.0
20	35.228503783217285	39.0	34.0	40.0	16.0	40.0
21	35.2527560718057	39.0	34.0	40.0	16.0	40.0
22	35.305337154925326	39.0	35.0	40.0	16.0	40.0
23	35.32483838953244	39.0	35.0	40.0	16.0	40.0
24	35.350580781415	39.0	35.0	40.0	16.0	40.0
25	35.37029829060508	39.0	35.0	40.0	27.0	40.0
26	35.37358814142008	39.0	35.0	40.0	27.0	40.0
27	35.354437519352764	39.0	35.0	40.0	16.0	40.0
28	35.23516700939254	39.0	34.0	40.0	16.0	40.0
29	35.31533945740804	39.0	35.0	40.0	16.0	40.0
30	35.329119419456774	39.0	35.0	40.0	16.0	40.0
31	35.31635191463347	39.0	35.0	40.0	16.0	40.0
32	35.25934180752832	39.0	35.0	40.0	16.0	40.0
33	35.14427229636922	39.0	34.0	40.0	16.0	40.0
34	35.187186445522464	39.0	34.0	40.0	16.0	40.0
35	35.183501202848724	39.0	34.0	40.0	16.0	40.0
36	35.19756127382871	39.0	34.0	40.0	16.0	40.0
37	35.17166898238204	39.0	34.0	40.0	16.0	40.0
38	35.18711340124334	39.0	34.0	40.0	16.0	40.0
39	35.14426181610309	39.0	34.0	40.0	16.0	40.0
40	35.059117910933615	39.0	34.0	40.0	16.0	40.0
41	35.03208295289438	39.0	34.0	40.0	16.0	40.0
42	35.04306246079825	39.0	34.0	40.0	16.0	40.0
43	34.99311065414328	39.0	34.0	40.0	16.0	40.0
44	34.87231193083024	39.0	34.0	40.0	16.0	40.0
45	34.88870084397901	39.0	34.0	40.0	16.0	40.0
46	34.850706703400526	39.0	34.0	40.0	16.0	40.0
47	34.774487856388596	39.0	34.0	40.0	16.0	40.0
48	34.82127462267072	39.0	34.0	40.0	16.0	40.0
49	34.76891584822669	39.0	34.0	40.0	16.0	40.0
50	34.66934029900517	39.0	34.0	40.0	16.0	40.0
51	34.703055632746064	39.0	34.0	40.0	16.0	40.0
52	34.73751252471199	39.0	34.0	40.0	16.0	40.0
53	34.67320180070027	39.0	34.0	40.0	16.0	40.0
54	34.702935586061244	39.0	34.0	40.0	16.0	40.0
55	34.690444061579505	39.0	34.0	40.0	16.0	40.0
56	34.64436550722106	39.0	34.0	40.0	16.0	40.0
57	34.523405133742486	39.0	34.0	40.0	16.0	40.0
58	34.544646410111866	39.0	34.0	40.0	16.0	40.0
59	34.49563052298116	39.0	34.0	40.0	16.0	40.0
60	34.46552675246723	39.0	34.0	40.0	16.0	40.0
61	34.44014068963327	39.0	34.0	40.0	16.0	40.0
62	34.39078911640241	39.0	34.0	40.0	16.0	40.0
63	34.361160768870434	39.0	34.0	40.0	16.0	40.0
64	34.35025081182365	39.0	34.0	40.0	16.0	40.0
65	34.27866964136847	39.0	34.0	40.0	16.0	40.0
66	34.230782764725966	39.0	34.0	40.0	16.0	40.0
67	34.22918976427341	39.0	34.0	40.0	16.0	40.0
68	34.190450889631684	39.0	34.0	40.0	16.0	40.0
69	34.156461481052155	39.0	34.0	40.0	16.0	40.0
70	34.0505437828997	39.0	34.0	40.0	16.0	40.0
71	33.94467848607792	39.0	34.0	40.0	16.0	40.0
72	33.93181253026971	39.0	34.0	40.0	16.0	40.0
73	33.88810569189606	39.0	34.0	40.0	16.0	40.0
74	33.844855221475015	39.0	34.0	40.0	16.0	40.0
75	33.75914379401513	39.0	34.0	40.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	7520.0
3	1638.0
4	1793.0
5	1852.0
6	1800.0
7	1920.0
8	2109.0
9	2295.0
10	2548.0
11	2684.0
12	3115.0
13	3520.0
14	3961.0
15	4471.0
16	5279.0
17	5955.0
18	7421.0
19	14200.0
20	26665.0
21	37137.0
22	43651.0
23	49346.0
24	53747.0
25	57828.0
26	62077.0
27	66513.0
28	71591.0
29	77776.0
30	84238.0
31	93086.0
32	103863.0
33	115504.0
34	132693.0
35	156247.0
36	196759.0
37	274524.0
38	487299.0
39	884150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.95454951110902	18.149039415852535	12.845286101344161	25.051124971694282
2	15.350128224468246	19.788203348921407	40.17546506180975	24.686203364800598
3	18.687807163103113	24.788941731308206	28.823971226905705	27.69927987868298
4	12.292780525760017	16.461163468332924	38.6111424284047	32.63491357750236
5	13.72937094584402	37.64987971512731	35.11594191391891	13.504807425109766
6	31.87766671165771	37.82140673754079	17.59436606299275	12.706560487808751
7	28.503021016109436	31.79468205889592	22.032981079943788	17.669315845050853
8	26.004462052703037	35.56027343967098	20.200649458916565	18.23461504870942
9	26.571920826353107	15.821200308056307	19.092853570039143	38.51402529555145
10	13.713428237965559	29.416423847369217	34.723852926931905	22.146294987733324
11	35.19241609832395	23.07230589673762	23.910981254614892	17.824296750323537
12	23.397035355019018	24.63443720176894	30.894458956260767	21.074068486951276
13	28.01594270787846	21.99436288715453	25.85846876960088	24.131225635366135
14	21.896451794745573	20.829655977324517	27.670697334677776	29.603194893252137
15	23.57929671062556	29.063905804638313	23.69003818945463	23.666759295281498
16	23.52251272320188	28.183245865455614	25.351350922184025	22.942890489158483
17	22.53003152019436	27.793316448460114	26.77222094306516	22.904431088280365
18	22.514946288636057	27.688704337401056	27.344888091400627	22.451461282562267
19	23.419488531254217	27.242721375773115	27.16215036006066	22.175639732912007
20	23.59412787512604	26.91891291057634	27.150050416431785	22.33690879786584
21	23.757905852275883	26.645473239593176	27.11067002246906	22.485950885661886
22	23.33650387849243	26.630737350239382	27.02095259267493	23.011806178593265
23	23.288771030003733	26.58354439424856	26.865273002993227	23.262411572754484
24	22.84866336908798	26.597454565664425	27.138458606918565	23.41542345832903
25	23.341267635826632	26.581321307492594	27.022064136052908	23.055346920627866
26	23.100634373685004	26.86330398329509	26.989098935300238	23.04696270771967
27	23.33491595938103	26.986081888988572	26.659859786742462	23.01914236488793
28	23.279703761063423	26.844253631543125	26.79517292525142	23.08086968214203
29	23.322498431929876	26.95533977499186	26.764630689712664	22.957531103365593
30	23.463029273288818	26.817571912886756	26.802550198092913	22.916848615731514
31	23.395669744583213	26.581321307492594	26.769553238958004	23.253455708966186
32	23.14747798747132	26.840088605886415	26.84783765115005	23.164595755492215
33	23.385204486955416	26.628110203692522	26.729384473608945	23.25730083574311
34	23.452957935598285	26.719982774209715	26.85384469539982	22.973214594792186
35	23.552666387827895	26.69652591660307	26.812729910984373	22.938077784584664
36	23.419273660161068	26.761907967990084	26.75244396708052	23.066374404768332
37	23.486138070508847	26.501984268132805	26.843864012845657	23.16801364851269
38	23.33343284378426	26.63509432955575	26.857341394103308	23.174131432556685
39	23.44633530924029	26.889748838036496	26.668487486363702	22.995428366359512
40	23.63853360069665	26.535851731499307	26.96376430953762	22.861850358266423
41	23.327251564507243	26.573328188442737	26.866682228372802	23.232738018677225
42	23.34354802391946	26.63010804663336	26.804051026238174	23.222292903209006
43	23.185595409514942	26.53658217452253	26.940326616009912	23.337495799952617
44	23.30807463279079	26.75080587534736	26.823755458515286	23.117364033346565
45	23.428398116065843	26.758756705084274	26.594785834288746	23.21805934456114
46	23.474800302848607	26.712765119564224	26.38832076545493	23.424113812132237
47	23.249102586374175	26.799440162526906	26.632486135066664	23.318971116032255
48	23.267669472847125	26.841103366273245	26.652330882848002	23.238896278031635
49	23.442352587723907	26.6214977208568	26.58958050617209	23.346569185247194
50	23.490169476022974	26.653601222574952	26.7065743891889	23.149654912213176
51	23.266526949892196	26.773600071901527	26.503747041287163	23.456125936919115
52	23.35579187395693	26.871646502676445	26.452148699569516	23.320412923797107
53	23.252027304699435	26.780556929993438	26.661208436839622	23.306207328467508
54	23.26493926791196	26.726660346527815	26.7233255998971	23.285074785663127
55	23.227360800886967	26.627420952523718	26.645333055547525	23.499885191041788
56	23.18966371438589	26.95325004954344	26.656719868697188	23.200366367373487
57	23.375644222670793	26.677456112826782	26.583704802785608	23.363194861716817
58	23.28007830413866	26.561668320233945	26.770195646015495	23.388057729611898
59	23.423476068417056	26.764709898889937	26.541637038479116	23.27017699421389
60	23.29006102586334	26.841026276215473	26.713607195430445	23.155305502490737
61	23.4796204493896	26.593251565501763	26.73162740724579	23.195500577862852
62	23.25971663834278	26.825985479072774	26.644894697711468	23.26940318487298
63	23.122079184180315	26.594997673668953	26.85259318424701	23.43032995790373
64	23.268244285282364	26.651850026296465	26.68443477841098	23.39547091001019
65	23.297185058713158	26.632341367765346	26.728031713425676	23.342441860095825
66	23.244961853831988	26.810807583373087	26.60033830070906	23.343892262085866
67	23.30084692068253	26.6926583375176	26.598460762072996	23.408033979726877
68	23.317048041874465	26.7870442211882	26.768496583772915	23.12741115316442
69	23.37060223943737	26.745479329540807	26.56047929142921	23.323439139592615
70	23.227868182376866	26.45823269410152	26.8034312142165	23.510467909305117
71	23.28677631670882	26.48153958923792	26.668538348084468	23.563145745968793
72	23.08773570134857	26.74268858569187	26.6623072523272	23.50726846063236
73	23.2247201038378	26.585093736988913	26.722481283884402	23.467704875288884
74	23.249898608595515	26.632131658604578	26.59036824532369	23.527601487476215
75	23.259998748697484	26.730076580349028	26.654680840049554	23.355243830903934
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	139.0
1	163.5
2	224.5
3	272.0
4	283.0
5	263.0
6	251.0
7	285.0
8	311.0
9	405.5
10	635.5
11	799.0
12	827.0
13	849.0
14	970.5
15	1223.0
16	1376.0
17	1525.5
18	1968.5
19	2709.0
20	3156.0
21	3940.0
22	6108.5
23	9188.5
24	10884.0
25	13744.5
26	20282.5
27	27636.5
28	31313.0
29	36352.5
30	45982.5
31	55723.5
32	60874.0
33	66203.0
34	77325.0
35	88622.0
36	94126.0
37	99254.0
38	109012.5
39	118019.5
40	122396.0
41	127523.5
42	136589.0
43	144774.0
44	149021.0
45	152354.0
46	157540.5
47	161261.0
48	163128.0
49	164675.0
50	164426.5
51	160743.5
52	158856.0
53	154661.5
54	145982.5
55	134502.5
56	127507.0
57	120946.5
58	105837.0
59	86946.5
60	76605.0
61	69638.0
62	54194.0
63	38935.0
64	32153.0
65	26572.0
66	17439.0
67	11616.5
68	9346.0
69	7804.0
70	5115.0
71	3177.5
72	2387.0
73	2043.0
74	1413.5
75	880.0
76	632.0
77	518.0
78	319.0
79	167.0
80	100.0
81	83.0
82	51.0
83	21.0
84	6.0
85	5.0
86	3.0
87	2.0
88	2.0
89	2.0
90	1.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0037474891029050978
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.09324261022143532
29	0.0
30	0.0
31	0.0
32	0.0
33	0.05954696667751745
34	2.5406705782407443E-4
35	6.351676445601861E-5
36	3.1758382228009304E-5
37	2.2230867559606512E-4
38	8.574763201562514E-4
39	3.1758382228009305E-4
40	3.1758382228009304E-5
41	6.351676445601861E-4
42	0.0013338520535763909
43	3.1758382228009304E-5
44	7.939595557002326E-4
45	0.0014291272002604188
46	2.2230867559606512E-4
47	1.2703352891203722E-4
48	3.4934220450810234E-4
49	1.2703352891203722E-4
50	3.4934220450810234E-4
51	7.622011734722234E-4
52	3.1758382228009305E-4
53	4.1285896896412096E-4
54	0.0034934220450810235
55	0.0020960532270486144
56	7.30442791244214E-4
57	6.034092623321768E-4
58	8.574763201562514E-4
59	8.892347023842605E-4
60	0.0034934220450810235
61	0.0033028717517129677
62	0.002477153813784726
63	0.0012703352891203722
64	0.0020007780803645864
65	0.0024453954315567165
66	0.0030805630761169024
67	0.0023501202848726886
68	0.004001556160729173
69	0.0040333145429571815
70	0.004827274098657414
71	0.0028900127827488466
72	0.0010797849957523164
73	5.716508801041675E-4
74	0.0027312208716088002
75	0.001778469404768521
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	3148775.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.28893936238153
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.63150357074258	49.13215522099231
2	12.383844068346193	15.675207126295005
3	4.43482469530888	8.420260536725875
4	2.0412227080150345	5.167472807707189
5	1.1166488180856693	3.533575966844946
6	0.6361748704520543	2.4157699679946596
7	0.40072396657170084	1.7752976374986567
8	0.28276858042642683	1.4316898832155864
9	0.1934443185285918	1.1018597170817939
>10	0.8361654344798158	8.907477827877576
>50	0.03303685385629114	1.4085573092543742
>100	0.00959137395300018	1.0131098655325532
>500	5.074123369976193E-5	0.017566132979497745
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	3.1758382228009304E-5	0.0
8	0.0	0.0	0.0	3.1758382228009304E-5	0.0
9	0.0	0.0	0.0	6.351676445601861E-5	0.0
10	0.0	0.0	0.0	6.351676445601861E-5	0.0
11	0.0	0.0	0.0	6.351676445601861E-5	0.0
12	0.0	0.0	0.0	6.351676445601861E-5	0.0
13	0.0	0.0	0.0	6.351676445601861E-5	0.0
14	0.0	0.0	0.0	6.351676445601861E-5	0.0
15	0.0	0.0	0.0	6.351676445601861E-5	0.0
16	0.0	0.0	0.0	6.351676445601861E-5	0.0
17	0.0	0.0	0.0	6.351676445601861E-5	0.0
18	0.0	0.0	0.0	6.351676445601861E-5	0.0
19	0.0	0.0	0.0	9.527514668402792E-5	0.0
20	0.0	0.0	0.0	9.527514668402792E-5	0.0
21	0.0	0.0	0.0	9.527514668402792E-5	0.0
22	0.0	0.0	0.0	9.527514668402792E-5	0.0
23	0.0	0.0	0.0	9.527514668402792E-5	3.1758382228009304E-5
24	0.0	0.0	0.0	1.2703352891203722E-4	3.1758382228009304E-5
25	0.0	0.0	0.0	1.5879191114004653E-4	3.1758382228009304E-5
26	0.0	0.0	0.0	1.5879191114004653E-4	3.1758382228009304E-5
27	0.0	0.0	0.0	2.2230867559606515E-4	3.1758382228009304E-5
28	0.0	0.0	0.0	5.081341156481489E-4	3.1758382228009304E-5
29	0.0	0.0	0.0	0.0012068185246643535	3.1758382228009304E-5
30	0.0	3.1758382228009304E-5	0.0	0.00228660352041667	3.1758382228009304E-5
31	0.0	3.1758382228009304E-5	0.0	0.006446951592285889	3.1758382228009304E-5
32	0.0	3.1758382228009304E-5	0.0	0.011528292748767377	3.1758382228009304E-5
33	0.0	3.1758382228009304E-5	0.0	0.01654611714079285	3.1758382228009304E-5
34	0.0	3.1758382228009304E-5	0.0	0.021468666386134292	3.1758382228009304E-5
35	0.0	3.1758382228009304E-5	0.0	0.026804074600439855	3.1758382228009304E-5
36	0.0	3.1758382228009304E-5	0.0	0.03477542853967019	3.1758382228009304E-5
37	0.0	3.1758382228009304E-5	0.0	0.044969869234861175	3.1758382228009304E-5
38	0.0	3.1758382228009304E-5	0.0	0.058625973592905174	3.1758382228009304E-5
39	0.0	3.1758382228009304E-5	0.0	0.07485450691141793	3.1758382228009304E-5
40	0.0	3.1758382228009304E-5	0.0	0.09174996625671888	3.1758382228009304E-5
41	0.0	3.1758382228009304E-5	0.0	0.1105826869179284	3.1758382228009304E-5
42	0.0	3.1758382228009304E-5	0.0	0.12814507229001754	3.1758382228009304E-5
43	0.0	3.1758382228009304E-5	0.0	0.1471365848623671	3.1758382228009304E-5
44	0.0	3.1758382228009304E-5	0.0	0.16615985581694467	3.1758382228009304E-5
45	0.0	3.1758382228009304E-5	0.0	0.18664401235401068	3.1758382228009304E-5
46	0.0	3.1758382228009304E-5	0.0	0.20903367182475724	3.1758382228009304E-5
47	0.0	3.1758382228009304E-5	0.0	0.2307246468864876	3.1758382228009304E-5
48	0.0	3.1758382228009304E-5	0.0	0.2539717826773904	3.1758382228009304E-5
49	0.0	3.1758382228009304E-5	0.0	0.2785210121396416	3.1758382228009304E-5
50	0.0	3.1758382228009304E-5	0.0	0.3074528983493581	3.1758382228009304E-5
51	0.0	3.1758382228009304E-5	0.0	0.33422521456756993	3.1758382228009304E-5
52	0.0	3.1758382228009304E-5	0.0	0.36128335622583385	3.1758382228009304E-5
53	0.0	3.1758382228009304E-5	0.0	0.39135854419575866	9.527514668402792E-5
54	0.0	3.1758382228009304E-5	0.0	0.4234980270105041	9.527514668402792E-5
55	0.0	3.1758382228009304E-5	0.0	0.45382728203825295	1.2703352891203722E-4
56	0.0	3.1758382228009304E-5	0.0	0.4864113822041905	1.2703352891203722E-4
57	0.0	3.1758382228009304E-5	0.0	0.5193130661924081	1.5879191114004653E-4
58	0.0	3.1758382228009304E-5	0.0	0.556406856634723	1.5879191114004653E-4
59	0.0	3.1758382228009304E-5	0.0	0.5954379083929464	1.5879191114004653E-4
60	0.0	3.1758382228009304E-5	0.0	0.6357392954402903	1.5879191114004653E-4
61	0.0	3.1758382228009304E-5	0.0	0.6780097021857706	1.5879191114004653E-4
62	0.0	3.1758382228009304E-5	0.0	0.7211693436336353	1.5879191114004653E-4
63	0.0	3.1758382228009304E-5	0.0	0.7656945955173043	1.5879191114004653E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3290	0.0	36.17018	1
ATCAACG	3925	0.0	29.176014	3
TCAACGC	3890	0.0	28.995173	4
CAACGCA	3980	0.0	28.599499	5
TATCAAC	4265	0.0	27.41626	2
AACGCAG	4115	0.0	27.409775	6
GGTATCA	1630	0.0	24.123747	1
ACGCAGA	4685	0.0	23.706846	7
CGCAGAG	5005	0.0	21.984375	8
GTACATG	6745	0.0	20.404148	1
AGAGTAC	5140	0.0	20.199047	11
TACATGG	6720	0.0	19.812786	2
ACATGGG	7085	0.0	17.867086	3
GAGTACT	2550	0.0	17.855059	12
CAGAGTA	5925	0.0	17.581102	10
TCGATCG	245	1.5927071E-8	16.895203	41
GCAGAGT	6320	0.0	16.864325	9
CGATCGT	275	3.741661E-9	16.30643	42
GTCTAGG	1070	0.0	16.118097	1
CATGGGG	5265	0.0	16.11626	4
>>END_MODULE