FastQCFastQC Report
Mon 6 Feb 2017
SRR3203967_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203967_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148775
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46550.039.115651
GGTATCA20800.033.01231
TCAACGC63200.027.8562764
CAACGCA63800.027.6906975
ATCAACG64200.027.4345873
CGCTATC12650.027.4240933
TATCAAC64850.027.3175132
TACGCTA15350.027.12080831
TATCTCG12650.026.60179736
AACGCAG66600.026.5777286
CGCTACG16450.026.5563128
ACGCTAT13800.026.14472232
TATGCCG17250.025.1673943
TCGTATG16900.024.8198240
GACGCTA18300.024.25057226
CCGTCTT16750.024.2103447
ACGCTAC18600.024.04583427
CTCGTAT16650.023.31904439
ACGCAGA74900.023.3030557
CGTATGC18850.023.17139441
Produced by FastQC (version 0.11.5)