FastQCFastQC Report
Mon 6 Feb 2017
SRR3203966_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203966_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4473137
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA39200.030.7136691
GGTATCA20950.024.700151
CAACGCA46250.024.388755
TCAACGC46550.024.3056764
ATCAACG47850.024.0057773
AACGCAG48250.023.6637846
TATCAAC52500.022.0109622
GTACATG85900.020.522021
TACATGG87900.019.386152
ACGCAGA56300.019.2999297
ACATGGG85850.018.9249233
CGAATCG4250.017.85632737
CGCAGAG60550.017.717398
CATGGGG66450.016.3519334
AGAGTAC63550.016.2839311
TGCGATA4900.016.19143313
CGTGCCG6650.016.08027810
GTATACG2705.2627E-815.332391
GCGATAC5250.015.11200414
GCACCGT10050.015.1021976
Produced by FastQC (version 0.11.5)