FastQCFastQC Report
Mon 6 Feb 2017
SRR3203966_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203966_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4473137
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60750.039.647931
GGTATCA25700.034.829391
ATCAACG80150.028.7577043
CAACGCA78750.028.7238545
TCAACGC79800.028.6155554
TATCAAC81950.028.3337612
AACGCAG82900.027.2859296
ACGCAGA94900.023.642417
CGCAGAG96750.022.8733948
GTACATG85100.021.7192821
AGAGTAC99900.021.13810511
CAGAGTA108350.020.0125310
TACATGG88450.019.9203112
GAGTACT69850.019.79707312
ACATGGG88500.019.0220643
GCAGAGT116650.018.8226059
AGTACTT76300.018.21407113
GTACTTT78500.017.70361314
CTCGTAT15950.016.95016739
TATGCCG16400.016.5915843
Produced by FastQC (version 0.11.5)