FastQCFastQC Report
Mon 6 Feb 2017
SRR3203965_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203965_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5837491
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55150.034.091
TCAACGC62500.028.5338574
ATCAACG64500.027.8630083
CAACGCA64850.027.6594375
GGTATCA27450.026.0138131
AACGCAG68400.025.9213126
TATCAAC72250.025.447162
ACGCAGA81050.021.2797727
GTACATG117650.020.4076651
CGCAGAG84550.019.7461198
TACATGG117700.019.5478652
ACATGGG121850.018.4574643
AGAGTAC84800.018.06080411
CATGGGG92200.016.5364134
CAGAGTA98250.015.76390310
GCAGAGT105500.014.9421719
CGTGCCG5550.014.91654510
GAGTACA84350.014.8046951
GTATAAT23400.014.7421261
GTATTAT19950.014.351961
Produced by FastQC (version 0.11.5)