FastQCFastQC Report
Mon 6 Feb 2017
SRR3203965_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203965_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5837491
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92000.041.1495441
GGTATCA37050.035.504681
TCAACGC119650.030.5218524
ATCAACG120100.030.5060863
CAACGCA121200.030.003955
TATCAAC125400.029.6511482
AACGCAG127800.028.561226
ACGCAGA142250.025.3595397
CGCAGAG145350.024.4200978
AGAGTAC148750.022.84001711
CAGAGTA162900.021.34562710
GAGTACT111750.021.07790212
GTACATG117400.020.7507861
GCAGAGT171100.020.4823789
AGTACTT119900.019.87496813
TACATGG121300.019.1126162
GTACTTT124250.018.76628514
ACATGGG122500.018.1178683
GTATTAG13200.017.342591
CGAATCG3850.017.23052837
Produced by FastQC (version 0.11.5)