FastQCFastQC Report
Mon 6 Feb 2017
SRR3203964_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203964_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4280017
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38700.034.851781
TCAACGC46600.027.906444
CAACGCA48050.027.1360995
ATCAACG48550.026.8566323
AACGCAG50400.025.8023836
TATCAAC52400.025.4758512
GGTATCA19300.022.8776721
GTACATG91650.022.7709981
ACGCAGA57950.022.0240557
TACATGG94600.021.4769782
ACATGGG94050.020.7590083
CGCAGAG60800.020.6512748
AGAGTAC65600.018.03596111
CATGGGG75550.016.8020534
GGACCGT4950.016.7245736
GCAGAGT77550.016.1463329
GAGTACA64000.016.0079461
CAGAGTA76400.015.93787410
GACCGTA2402.3171742E-715.8099487
GTCTTAG12900.015.5094871
Produced by FastQC (version 0.11.5)