FastQCFastQC Report
Mon 6 Feb 2017
SRR3203964_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203964_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4280017
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58050.039.5407451
GGTATCA24650.031.731921
ATCAACG76250.029.0521133
TCAACGC76100.028.7832854
CAACGCA77500.028.0359975
TATCAAC79650.027.9817522
AACGCAG80250.027.160596
GTACATG89650.024.4222431
CTCGTAT15650.022.81405439
ACGCAGA94850.022.7536897
TACATGG93150.022.4241542
TATGCCG17000.022.2948843
CGCAGAG98450.021.7485038
TCGTATG17300.021.6440
AATCTCG14700.021.00131436
ACGTAGA18950.020.48966427
ACATGGG97100.020.4586353
AGAGTAC101850.020.36178211
GACGTAG18550.020.18393526
TCTCGTA15750.020.02860838
Produced by FastQC (version 0.11.5)