FastQCFastQC Report
Mon 6 Feb 2017
SRR3203963_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203963_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4404805
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43800.035.519511
GGTATCA21150.030.336611
TCAACGC52300.028.228764
CAACGCA52200.028.0845955
ATCAACG54300.026.998453
TATCAAC56550.026.5342242
AACGCAG54350.026.4658746
GTACATG101200.023.7924631
TACATGG106100.022.1401942
ACGCAGA64150.021.8312747
ACATGGG103300.021.7051493
CGCAGAG66500.020.8523068
CATGGGG74050.018.7262464
AGAGTAC70000.018.2820811
CGCACGA4600.016.49736435
CAGAGTA79750.016.17672510
TATAGCG2001.1451557E-515.522525
GCAGAGT85100.015.4840129
GAGTACA70750.014.8709221
GTATTAA26550.014.2919881
Produced by FastQC (version 0.11.5)