FastQCFastQC Report
Mon 6 Feb 2017
SRR3203963_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203963_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4404805
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70100.040.5005871
GGTATCA27600.037.754531
ATCAACG91350.030.5400373
TCAACGC91250.030.5218544
CAACGCA91800.030.21445
TATCAAC93150.030.0944712
AACGCAG95700.028.9474456
ACGCAGA112200.024.647747
CGCAGAG114700.023.8132278
CTCGTAT13800.022.35394739
TCGTATG14450.022.3073740
AGAGTAC115750.022.12950111
TATGCCG13750.022.05284343
GTACATG100350.021.959411
GACGTAG14900.021.8715826
GAGTACT78850.021.71206712
AATCTCG11850.021.07284536
CGTAGAG15750.020.69096828
TACATGG103550.020.5430242
CAGAGTA129050.020.23630110
Produced by FastQC (version 0.11.5)