FastQCFastQC Report
Mon 6 Feb 2017
SRR3203962_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203962_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2359674
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23300.041.454531
TCAACGC30850.030.3022424
CAACGCA31250.030.024765
TATCAAC32350.029.3237232
ATCAACG31450.029.2854083
AACGCAG33850.027.7185766
GGTATCA12150.025.5526581
ACGCAGA39350.023.2306757
GTATATA4500.021.4642331
CGCAGAG40950.021.3963898
GTACATG60650.021.2152371
TACATGG62050.020.2357962
AGAGTAC41100.019.30397211
ACATGGG65450.018.7102413
GTACTAA3750.018.3979151
CTAATAC3250.018.0437263
CGTGCCG4400.018.03166610
CATGGGG53400.017.9582584
CAGAGTA46350.017.63840510
CGGTATG3501.8189894E-1216.75488959
Produced by FastQC (version 0.11.5)