##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3203962_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2359674
Sequences flagged as poor quality	0
Sequence length	75
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.46522316218257	35.0	31.0	35.0	15.0	35.0
2	31.418861673265035	35.0	31.0	35.0	15.0	35.0
3	31.401691504843466	35.0	31.0	35.0	15.0	35.0
4	31.57766284664746	35.0	31.0	35.0	15.0	35.0
5	31.622128310944646	35.0	31.0	35.0	15.0	35.0
6	35.244344345871504	39.0	34.0	40.0	16.0	40.0
7	35.023658352806365	39.0	34.0	40.0	16.0	40.0
8	34.91226584689241	39.0	34.0	40.0	16.0	40.0
9	34.938291899643765	39.0	34.0	40.0	16.0	40.0
10	35.0490542337628	39.0	34.0	40.0	16.0	40.0
11	35.122439370862246	39.0	34.0	40.0	16.0	40.0
12	34.6967538736283	39.0	34.0	40.0	16.0	40.0
13	34.74737823953648	39.0	34.0	40.0	16.0	40.0
14	34.70784608382344	39.0	34.0	40.0	16.0	40.0
15	34.76615371445378	39.0	34.0	40.0	16.0	40.0
16	34.90419905461517	39.0	34.0	40.0	16.0	40.0
17	34.937741399871335	39.0	34.0	40.0	16.0	40.0
18	34.994567469913214	39.0	34.0	40.0	16.0	40.0
19	35.02983166318737	39.0	34.0	40.0	16.0	40.0
20	34.9880072416783	39.0	34.0	40.0	16.0	40.0
21	35.00179262050605	39.0	34.0	40.0	16.0	40.0
22	35.05089728496394	39.0	34.0	40.0	16.0	40.0
23	35.06237937952446	39.0	34.0	40.0	16.0	40.0
24	35.08500581012462	39.0	34.0	40.0	16.0	40.0
25	35.09945907782177	39.0	34.0	40.0	16.0	40.0
26	35.10207681230543	39.0	34.0	40.0	16.0	40.0
27	35.095750938477096	39.0	34.0	40.0	16.0	40.0
28	34.96374753461707	39.0	34.0	40.0	16.0	40.0
29	35.05722527772904	39.0	34.0	40.0	16.0	40.0
30	35.0697198002775	39.0	34.0	40.0	16.0	40.0
31	35.05764228448506	39.0	34.0	40.0	16.0	40.0
32	34.99431192613895	39.0	34.0	40.0	16.0	40.0
33	34.88547019630678	39.0	34.0	40.0	16.0	40.0
34	34.91760260103726	39.0	34.0	40.0	16.0	40.0
35	34.91056645960417	39.0	34.0	40.0	16.0	40.0
36	34.92214687283074	39.0	34.0	40.0	16.0	40.0
37	34.895424537457295	39.0	34.0	40.0	16.0	40.0
38	34.91234467134019	39.0	34.0	40.0	16.0	40.0
39	34.879713892681785	39.0	34.0	40.0	16.0	40.0
40	34.79848445166578	39.0	34.0	40.0	16.0	40.0
41	34.758866690907304	39.0	34.0	40.0	16.0	40.0
42	34.77517106176531	39.0	34.0	40.0	16.0	40.0
43	34.73369838375979	39.0	34.0	40.0	16.0	40.0
44	34.61406109488006	39.0	34.0	40.0	16.0	40.0
45	34.639075991005534	39.0	34.0	40.0	16.0	40.0
46	34.61009359767493	39.0	34.0	40.0	16.0	40.0
47	34.525083125889424	39.0	34.0	40.0	16.0	40.0
48	34.562078490503346	39.0	34.0	40.0	16.0	40.0
49	34.5104311019234	39.0	34.0	40.0	16.0	40.0
50	34.410415591306254	39.0	34.0	40.0	16.0	40.0
51	34.4511644405117	39.0	34.0	40.0	16.0	40.0
52	34.480655802454066	39.0	34.0	40.0	16.0	40.0
53	34.425886372439585	39.0	34.0	40.0	16.0	40.0
54	34.45559979895528	39.0	34.0	40.0	16.0	40.0
55	34.44556154790874	39.0	34.0	40.0	16.0	40.0
56	34.38632751812327	39.0	34.0	40.0	16.0	40.0
57	34.26743439983659	39.0	34.0	40.0	16.0	40.0
58	34.28810250907541	39.0	34.0	40.0	16.0	40.0
59	34.232076125769915	39.0	34.0	40.0	16.0	40.0
60	34.205289798506065	39.0	34.0	40.0	16.0	40.0
61	34.17036675405162	39.0	34.0	40.0	16.0	40.0
62	34.126543751382606	39.0	34.0	40.0	16.0	40.0
63	34.09653113099522	39.0	34.0	40.0	16.0	40.0
64	34.072952873998695	39.0	34.0	40.0	16.0	40.0
65	33.99528367054093	39.0	34.0	40.0	16.0	40.0
66	33.95357748570353	39.0	34.0	40.0	16.0	40.0
67	33.941613968709234	39.0	34.0	40.0	16.0	40.0
68	33.89926362709425	39.0	34.0	40.0	16.0	40.0
69	33.86439991286932	39.0	34.0	40.0	16.0	40.0
70	33.75322438608045	39.0	34.0	40.0	16.0	40.0
71	33.652165934785906	39.0	34.0	40.0	16.0	40.0
72	33.62165960213148	39.0	34.0	40.0	16.0	40.0
73	33.57339954586947	39.0	34.0	40.0	16.0	40.0
74	33.52047231948142	39.0	34.0	40.0	16.0	40.0
75	33.421635785282206	39.0	34.0	40.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5484.0
3	1528.0
4	1735.0
5	1739.0
6	1658.0
7	1788.0
8	1896.0
9	2072.0
10	2265.0
11	2516.0
12	2737.0
13	3177.0
14	3664.0
15	4272.0
16	4836.0
17	5485.0
18	6600.0
19	11954.0
20	21284.0
21	29566.0
22	34295.0
23	38804.0
24	42216.0
25	45545.0
26	48969.0
27	51842.0
28	56441.0
29	60467.0
30	65321.0
31	71044.0
32	79398.0
33	87891.0
34	99945.0
35	118827.0
36	148117.0
37	204924.0
38	356569.0
39	632803.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.37634663796099	15.28377931665805	12.231371684791362	26.108502360589597
2	16.247837625027863	18.189080356015282	38.329574339506216	27.233507679450636
3	20.02912266694467	22.511626606048125	26.973217486822332	30.486033240184874
4	13.54835456084188	15.211677545288035	35.0220835590001	36.217884334869986
5	15.838289526434584	35.98441988172942	32.682862124174775	15.494428467661212
6	35.33462673233675	33.95028296281605	16.495626090722702	14.219464214124494
7	31.342931269319408	29.845309140160886	19.5016769265585	19.310082663961207
8	27.994163600565162	33.477421033583454	18.806072364233366	19.72234300161802
9	27.745103772809294	15.065089499651224	17.14211369875669	40.04769302878279
10	15.46171208395736	28.019675599256505	32.04976619651698	24.468846120269156
11	37.44101939505203	21.450590208647466	21.724865383947105	19.3835250123534
12	25.466272035882927	22.987031259402784	28.308359544581158	23.23833716013314
13	29.93591487637699	20.064847940859625	23.99395001173891	26.00528717102447
14	23.872789207322707	19.046656444915698	25.060198993589793	32.020355354171805
15	25.42791928037517	26.70936748042314	21.97706123812018	25.885652001081503
16	25.840900056533233	25.87077706496745	23.224987858492316	25.063335020007
17	24.788085133793906	25.707364661389665	24.49198490977991	25.012565295036516
18	24.801264920493253	25.670071374266108	24.782236868313166	24.746426836927473
19	25.776145348891415	25.20127780362881	24.698920274580303	24.323656572899477
20	25.885863894758344	24.768718051730875	24.8476272569855	24.497790796525283
21	26.119370726634273	24.44994520429517	24.86173937586294	24.56894469320762
22	25.788435182148046	24.337048253275665	24.66620389087645	25.20831267369984
23	25.583533996645297	24.429391517641843	24.573394460421227	25.41368002529163
24	25.215771331124554	24.44316460663634	24.783677745315664	25.55738631692344
25	25.60256204882539	24.28305774441724	24.622511414712374	25.491868792045004
26	25.369818034186075	24.81126630204003	24.71926206755679	25.099653596217102
27	25.476654826048005	24.876953341859934	24.49859599249727	25.147795839594796
28	25.590994737569915	24.708606614050847	24.406205646232014	25.29419300214722
29	25.68121698166781	24.868011428697354	24.382774908737392	25.067996680897448
30	25.606588028685316	24.74833388001902	24.49486666378491	25.150211427510747
31	25.589255125919934	24.42650976363684	24.324631283812934	25.65960382663029
32	25.426266509695832	24.75566540123763	24.50804645048426	25.31002163858228
33	25.643569519753857	24.45155034569192	24.436965440983496	25.467914693570727
34	25.768739990668198	24.65091561415501	24.478815496893002	25.10152889828379
35	25.738470653149548	24.51817496823714	24.464862519144596	25.278491859468723
36	25.58883133856626	24.767573825875946	24.38561428400703	25.257980551550762
37	25.71517324646254	24.35807842866363	24.490342517798016	25.43640580707581
38	25.63632156551633	24.661137047095	24.508275782571697	25.194265604816973
39	25.719550377425975	24.769798124142888	24.404873905990165	25.10577759244097
40	25.81297246992593	24.434137936003026	24.64264131401202	25.11024828005903
41	25.46147401307049	24.50837342726749	24.69683222251275	25.333320337149267
42	25.525245162438658	24.53730622208284	24.634396480848935	25.303052134629567
43	25.376281110256	24.373684139151543	24.64728148657949	25.60275326401296
44	25.546943203681888	24.66774874346304	24.46043074002187	25.324877312833205
45	25.51215266005777	24.663254858351756	24.39804581022254	25.426546671367934
46	25.579667674010487	24.515451518453208	24.246473343105883	25.65840746443042
47	25.44887594547039	24.686439471248548	24.414325380815637	25.450359202465428
48	25.30591141981718	24.744943148830135	24.59250657930982	25.356638852042874
49	25.74475361628497	24.366977966862358	24.339770807226255	25.54849760962642
50	25.769885522079576	24.46186308333923	24.453472075956416	25.314779318624776
51	25.422359040252786	24.751288637402883	24.39276286486672	25.433589457477613
52	25.63755710012116	24.69293388781741	24.193372720180058	25.476136291881367
53	25.40946438227751	24.7245798985281	24.412968265027114	25.452987454167282
54	25.25699271062892	24.760764536362096	24.4568994744872	25.525343278521785
55	25.50036297955999	24.42833368013809	24.35010130795542	25.7212020323465
56	25.23404845017992	24.849967855552133	24.51644537075453	25.399538323513422
57	25.62442179243392	24.525324167052666	24.331906717188005	25.518347323325408
58	25.465161762505918	24.492861904432964	24.445906046753286	25.59607028630783
59	25.573312799327702	24.70242572774163	24.4414986377292	25.282762835201467
60	25.525467746392206	24.72037775702107	24.570225952402343	25.18392854418438
61	25.67515680623835	24.441430750974742	24.486862180030514	25.3965502627564
62	25.34254325574063	24.913747033961695	24.39718277746205	25.346526932835626
63	25.22563718109161	24.625548439154773	24.54329051202911	25.605523867724507
64	25.481768405349968	24.529970673492567	24.460171890627382	25.528089030530083
65	25.573956266890885	24.554983385114028	24.388982853675003	25.482077494320087
66	25.377159784879705	24.612118104262994	24.556303795966283	25.45441831489102
67	25.560311965089337	24.468061211473188	24.32219045161028	25.649436371827193
68	25.43965227886855	24.808568599995255	24.523349799456856	25.22842932167934
69	25.490476119842597	24.622318660617605	24.377742790606018	25.509462428933777
70	25.36495994615977	24.416992219782045	24.64919515132816	25.56885268273002
71	25.494870334394527	24.474870402202402	24.530133818837704	25.50012544456537
72	25.229059200254955	24.7525058779854	24.463014037663182	25.55542088409647
73	25.425230032750424	24.49035116864096	24.479629303155026	25.604789495453588
74	25.445738955535656	24.69570325064915	24.41277645859229	25.4457813352229
75	25.356695604635444	24.716895973758763	24.43401443861259	25.492393982993207
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	121.0
1	151.0
2	218.0
3	260.0
4	265.0
5	254.5
6	243.0
7	266.0
8	290.0
9	378.0
10	537.0
11	613.5
12	619.0
13	641.5
14	723.0
15	878.0
16	974.0
17	1037.0
18	1249.5
19	1530.5
20	1662.0
21	1917.5
22	2466.0
23	3071.0
24	3383.0
25	3739.5
26	4701.5
27	5698.5
28	6090.0
29	6755.5
30	8147.5
31	9779.0
32	10684.0
33	11698.5
34	14321.0
35	17994.0
36	20059.0
37	23071.5
38	30665.5
39	41446.0
40	47645.0
41	55877.0
42	72372.5
43	89632.5
44	98629.0
45	107589.5
46	123346.5
47	136452.5
48	142762.0
49	148431.0
50	156745.5
51	160623.0
52	161855.0
53	160685.5
54	156748.5
55	148697.5
56	143414.0
57	136954.0
58	121359.0
59	101619.0
60	91014.0
61	82199.0
62	63840.0
63	46687.0
64	39078.0
65	32292.0
66	21127.0
67	14338.5
68	11929.0
69	9889.5
70	6454.0
71	4139.5
72	3221.0
73	2802.5
74	1956.5
75	1127.0
76	725.0
77	550.5
78	309.0
79	175.0
80	108.0
81	76.5
82	31.5
83	15.0
84	12.0
85	7.5
86	3.0
87	2.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.003983601124562122
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.09357224769184218
29	0.0
30	0.0
31	0.0
32	0.0
33	0.045387625578787574
34	4.2378735367682146E-5
35	0.0
36	0.0
37	4.2378735367682146E-5
38	7.628172366182786E-4
39	2.542724122060929E-4
40	0.0
41	2.96651147573775E-4
42	5.9330229514755E-4
43	8.475747073536429E-5
44	5.9330229514755E-4
45	0.0011018471195597358
46	1.2713620610304644E-4
47	8.475747073536429E-5
48	1.6951494147072858E-4
49	4.2378735367682146E-5
50	2.118936768384107E-4
51	4.661660890445036E-4
52	1.2713620610304644E-4
53	2.542724122060929E-4
54	0.003136026417208479
55	0.0015680132086042394
56	5.509235597798679E-4
57	4.661660890445036E-4
58	4.661660890445036E-4
59	4.661660890445036E-4
60	0.002627481592796293
61	0.003136026417208479
62	0.0019070430915456966
63	9.747109134566895E-4
64	0.001440877002501193
65	0.001822285620810332
66	0.0027122390635316573
67	0.0021613155037517894
68	0.003475056300149936
69	0.0033479200940468895
70	0.004237873536768215
71	0.0027969965342670216
72	9.323321780890072E-4
73	4.237873536768214E-4
74	0.0022460729744871537
75	0.0012289833256627822
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	2359674.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.38707958997188
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.30647644547082	52.510059669302024
2	11.09431683309964	14.508499555245017
3	3.8214930383347103	7.496288083503456
4	1.7279491242804634	4.519421876669956
5	0.8938412142251941	2.922283330766994
6	0.5345459041812842	2.097143734871707
7	0.3521004656005348	1.611597481752183
8	0.2487962915930569	1.301445033606805
9	0.17797676010002306	1.0473642520042983
>10	0.7918993688767415	8.901909003678174
>50	0.03830227399390367	1.7058910733057941
>100	0.011908540996035962	1.2229376025542644
>500	3.9373924767341335E-4	0.15515930273923667
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	4.2378735367682146E-5
3	0.0	0.0	0.0	0.0	4.2378735367682146E-5
4	0.0	0.0	0.0	0.0	4.2378735367682146E-5
5	0.0	0.0	0.0	0.0	4.2378735367682146E-5
6	0.0	0.0	0.0	0.0	1.2713620610304644E-4
7	0.0	0.0	0.0	0.0	1.2713620610304644E-4
8	0.0	0.0	0.0	0.0	1.2713620610304644E-4
9	0.0	0.0	0.0	0.0	2.1189367683841073E-4
10	0.0	0.0	0.0	0.0	2.1189367683841073E-4
11	0.0	0.0	0.0	0.0	2.1189367683841073E-4
12	0.0	0.0	0.0	0.0	2.1189367683841073E-4
13	0.0	0.0	0.0	0.0	2.1189367683841073E-4
14	0.0	0.0	0.0	0.0	2.96651147573775E-4
15	0.0	0.0	0.0	0.0	3.814086183091393E-4
16	0.0	0.0	0.0	0.0	3.814086183091393E-4
17	0.0	0.0	0.0	0.0	3.814086183091393E-4
18	0.0	0.0	0.0	0.0	4.2378735367682146E-4
19	0.0	0.0	0.0	0.0	4.2378735367682146E-4
20	0.0	0.0	0.0	0.0	4.2378735367682146E-4
21	0.0	0.0	0.0	0.0	4.661660890445036E-4
22	0.0	0.0	0.0	4.2378735367682146E-5	4.661660890445036E-4
23	0.0	0.0	0.0	4.2378735367682146E-5	5.9330229514755E-4
24	0.0	0.0	0.0	4.2378735367682146E-5	5.9330229514755E-4
25	0.0	0.0	0.0	4.2378735367682146E-5	5.9330229514755E-4
26	0.0	0.0	0.0	1.2713620610304644E-4	5.9330229514755E-4
27	0.0	0.0	0.0	2.542724122060929E-4	5.9330229514755E-4
28	0.0	0.0	0.0	7.204385012505965E-4	5.9330229514755E-4
29	0.0	0.0	0.0	0.001483255737868875	5.9330229514755E-4
30	0.0	0.0	0.0	0.0028393752696347038	5.9330229514755E-4
31	0.0	0.0	0.0	0.006483946511255369	6.780597658829143E-4
32	0.0	0.0	0.0	0.01084895625412663	6.780597658829143E-4
33	0.0	0.0	0.0	0.015425859673836301	6.780597658829143E-4
34	0.0	0.0	0.0	0.01957897573986915	6.780597658829143E-4
35	0.0	0.0	0.0	0.024537287777887964	7.204385012505965E-4
36	0.0	0.0	0.0	0.03140264290745247	7.204385012505965E-4
37	0.0	0.0	0.0	0.040429313540768765	7.204385012505965E-4
38	0.0	0.0	0.0	0.05348196403401487	7.204385012505965E-4
39	0.0	0.0	0.0	0.06920447485542494	7.628172366182786E-4
40	0.0	0.0	0.0	0.0847574707353643	7.628172366182786E-4
41	0.0	0.0	0.0	0.10094614764581887	7.628172366182786E-4
42	0.0	0.0	0.0	0.12027085097348193	7.628172366182786E-4
43	0.0	0.0	0.0	0.13895987327062975	7.628172366182786E-4
44	4.2378735367682146E-5	0.0	0.0	0.15896263636417574	7.628172366182786E-4
45	4.2378735367682146E-5	0.0	0.0	0.17930442934066315	8.051959719859608E-4
46	4.2378735367682146E-5	0.0	0.0	0.2005785544952396	8.051959719859608E-4
47	4.2378735367682146E-5	0.0	0.0	0.2247768123901861	8.051959719859608E-4
48	4.2378735367682146E-5	0.0	0.0	0.24889031281439725	9.747109134566894E-4
49	4.2378735367682146E-5	0.0	0.0	0.2745718264472126	9.747109134566894E-4
50	4.2378735367682146E-5	0.0	0.0	0.3036012601740749	9.747109134566894E-4
51	4.2378735367682146E-5	0.0	0.0	0.33144408931064206	9.747109134566894E-4
52	4.2378735367682146E-5	0.0	0.0	0.3596683270655184	9.747109134566894E-4
53	4.2378735367682146E-5	0.0	0.0	0.3914947573266477	0.0012713620610304644
54	4.2378735367682146E-5	0.0	0.0	0.42378735367682147	0.0012713620610304644
55	4.2378735367682146E-5	0.0	0.0	0.4555290264672154	0.0012713620610304644
56	4.2378735367682146E-5	0.0	0.0	0.4914661940590098	0.0012713620610304644
57	4.2378735367682146E-5	0.0	0.0	0.5274881191215397	0.0013561195317658287
58	4.2378735367682146E-5	0.0	0.0	0.5661799045122334	0.0013561195317658287
59	4.2378735367682146E-5	0.0	0.0	0.6079229588494004	0.0013984982671335108
60	4.2378735367682146E-5	0.0	0.0	0.648776059743846	0.0013984982671335108
61	4.2378735367682146E-5	0.0	0.0	0.6921718847603525	0.0013984982671335108
62	4.2378735367682146E-5	0.0	0.0	0.7367119356317864	0.001483255737868875
63	4.2378735367682146E-5	0.0	0.0	0.7850236939509441	0.001483255737868875
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2330	0.0	41.45453	1
TCAACGC	3085	0.0	30.302242	4
CAACGCA	3125	0.0	30.02476	5
TATCAAC	3235	0.0	29.323723	2
ATCAACG	3145	0.0	29.285408	3
AACGCAG	3385	0.0	27.718576	6
GGTATCA	1215	0.0	25.552658	1
ACGCAGA	3935	0.0	23.230675	7
GTATATA	450	0.0	21.464233	1
CGCAGAG	4095	0.0	21.396389	8
GTACATG	6065	0.0	21.215237	1
TACATGG	6205	0.0	20.235796	2
AGAGTAC	4110	0.0	19.303972	11
ACATGGG	6545	0.0	18.710241	3
GTACTAA	375	0.0	18.397915	1
CTAATAC	325	0.0	18.043726	3
CGTGCCG	440	0.0	18.031666	10
CATGGGG	5340	0.0	17.958258	4
CAGAGTA	4635	0.0	17.638405	10
CGGTATG	350	1.8189894E-12	16.754889	59
>>END_MODULE