FastQCFastQC Report
Mon 6 Feb 2017
SRR3203962_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203962_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2359674
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29850.042.9327771
GGTATCA13100.035.217991
TATCAAC43650.029.4172062
CAACGCA43450.028.5066495
TCAACGC43900.028.148654
ATCAACG45700.027.7251533
AACGCAG46000.026.9263886
ACGCAGA54350.022.7171697
CTCGTAT10000.021.49077639
GTACATG57000.021.463981
CGCAGAG58050.021.0929538
TGCGATA3250.021.06400713
TCGTATG10550.020.69851140
AATCTCG9200.020.35768336
TCTCGTA9450.020.174438
GCGATAC3250.020.0112314
TATGCCG10900.019.90951743
AGAGTAC58500.019.83009311
TACATGG59000.019.7957482
GTACTTT38650.019.12968614
Produced by FastQC (version 0.11.5)