FastQCFastQC Report
Mon 6 Feb 2017
SRR3203961_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203961_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643077
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA35450.036.4935341
TCAACGC43300.029.1572384
CAACGCA44250.028.6871725
ATCAACG43700.028.4956743
AACGCAG45900.027.6559336
TATCAAC47200.026.9673042
GGTATCA17550.025.1613371
ACGCAGA54450.022.9331577
GTACATG78850.022.0510731
CGCAGAG57600.021.2597928
TACATGG80850.021.2045732
AGAGTAC58200.019.67742511
ACATGGG83400.019.6049393
GAGTACT28950.017.87293412
CAGAGTA67350.017.77235810
GTATACG2156.6065695E-817.6504231
GCAGAGT70650.017.0398759
CATGGGG60500.016.9337924
TCGCGTC5250.016.42607917
GTATATA15300.016.009131
Produced by FastQC (version 0.11.5)