FastQCFastQC Report
Mon 6 Feb 2017
SRR3203961_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203961_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3643077
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56500.041.7584721
GGTATCA23250.033.580391
TCAACGC78600.029.1633994
ATCAACG79100.029.0330753
CAACGCA78900.028.998795
TATCAAC80600.028.8745272
AACGCAG81950.027.9612526
ACGCAGA93850.024.042827
CGCAGAG97400.022.9910138
AGAGTAC95500.022.420111
GAGTACT67950.021.0429212
GTACATG76700.020.804841
CAGAGTA105300.020.77627810
TATGCCG14350.020.39332243
AGTACTT72800.019.73603813
GCAGAGT111450.019.691159
TACATGG78400.019.644342
AATCTCG12200.019.60696436
TCGTATG15550.019.13752740
CGCACGA4400.018.90948335
Produced by FastQC (version 0.11.5)