FastQCFastQC Report
Mon 6 Feb 2017
SRR3203960_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203960_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3618439
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36150.036.0714571
CAACGCA45200.028.236675
TCAACGC45100.028.146314
ATCAACG45750.027.5956213
AACGCAG47150.026.9225586
GGTATCA17950.026.7134861
TATCAAC48750.025.968192
ACGCAGA54100.022.7625647
CGCAGAG57650.021.0617078
GTACATG76100.020.9882661
TACATGG77350.019.9341352
AGAGTAC57150.019.25416211
GCGATAC3350.018.53437214
GTGCGAT3950.018.33886512
ACATGGG82000.017.8782633
GTATACG1552.2499331E-517.8048571
TGCGATA3350.017.50468413
CAGAGTA65350.016.99653810
GCAGAGT69300.016.4259669
GTATAGG9150.016.2116621
Produced by FastQC (version 0.11.5)