FastQCFastQC Report
Mon 6 Feb 2017
SRR3203960_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203960_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3618439
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55650.041.3759571
GGTATCA24250.033.3879621
TCAACGC75350.029.6236784
CAACGCA74700.029.5946125
TATCAAC76850.029.5911052
ATCAACG76650.029.3123463
AACGCAG78800.028.1417686
ACGCAGA89850.024.3236057
TCGTATG17200.023.78213140
TATGCCG17950.023.42137343
CTCGTAT16050.023.32630339
CGCAGAG93850.023.1419098
AGAGTAC94700.021.86134111
GTACATG76700.021.7359091
CGTATGC20850.021.61396841
TCTCGTA15300.021.52462238
GAGTACT68300.021.1544912
AGTACTT72150.020.50219513
CAGAGTA102100.020.46542210
ATGCCGT19800.020.1879244
Produced by FastQC (version 0.11.5)