FastQCFastQC Report
Mon 6 Feb 2017
SRR3203959_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203959_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2992164
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37350.041.007141
GGTATCA14750.032.975711
TCAACGC48900.029.8384914
ATCAACG49050.029.8175663
TATCAAC50800.029.5373062
CAACGCA50400.028.745125
AACGCAG53000.027.2698976
GTACATG96000.022.8894481
ACGCAGA62300.022.5346977
TACATGG97200.021.647562
CGCAGAG65000.021.4925048
ACATGGG97600.020.1451453
AGAGTAC65600.019.87619811
CAGAGTA73900.018.15726910
CATGGGG61650.018.1283074
GCAGAGT75000.017.8449699
ATACCGT2952.910383E-1117.5393946
GAGTACT27150.016.64358312
GAGTACA65600.015.7755521
GGACCGT3502.1827873E-1115.7687516
Produced by FastQC (version 0.11.5)