##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3203959_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2992164
Sequences flagged as poor quality	0
Sequence length	75
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.07305916386936	35.0	31.0	35.0	15.0	35.0
2	31.037615919448264	35.0	31.0	35.0	15.0	35.0
3	30.93157326937962	35.0	31.0	35.0	15.0	35.0
4	31.34373750904028	35.0	31.0	35.0	15.0	35.0
5	31.223596367044053	35.0	31.0	35.0	15.0	35.0
6	34.780376677214214	39.0	34.0	40.0	16.0	40.0
7	34.42252396593235	39.0	34.0	40.0	16.0	40.0
8	34.43199804556168	39.0	34.0	40.0	16.0	40.0
9	34.4388506111296	39.0	34.0	40.0	16.0	40.0
10	34.54008069076428	39.0	34.0	40.0	16.0	40.0
11	34.59460143227444	39.0	34.0	40.0	16.0	40.0
12	34.193106728107146	39.0	31.0	40.0	16.0	40.0
13	34.24654297023826	39.0	34.0	40.0	16.0	40.0
14	34.214750261015105	39.0	31.0	40.0	16.0	40.0
15	34.278132147836814	39.0	34.0	40.0	16.0	40.0
16	34.414879665686776	39.0	34.0	40.0	16.0	40.0
17	34.45477320093417	39.0	34.0	40.0	16.0	40.0
18	34.50013735878114	39.0	34.0	40.0	16.0	40.0
19	34.53564477080802	39.0	34.0	40.0	16.0	40.0
20	34.500738595879106	39.0	34.0	40.0	16.0	40.0
21	34.515939300118575	39.0	34.0	40.0	16.0	40.0
22	34.569243530769036	39.0	34.0	40.0	16.0	40.0
23	34.57184933713526	39.0	34.0	40.0	16.0	40.0
24	34.596531139335944	39.0	34.0	40.0	16.0	40.0
25	34.61746949699281	39.0	34.0	40.0	16.0	40.0
26	34.626396146735274	39.0	34.0	40.0	16.0	40.0
27	34.62019361238221	39.0	34.0	40.0	16.0	40.0
28	34.49237575213123	39.0	34.0	40.0	16.0	40.0
29	34.57470312456135	39.0	34.0	40.0	16.0	40.0
30	34.59493363331689	39.0	34.0	40.0	16.0	40.0
31	34.58507053757749	39.0	34.0	40.0	16.0	40.0
32	34.52594543614588	39.0	34.0	40.0	16.0	40.0
33	34.414302157234694	39.0	34.0	40.0	16.0	40.0
34	34.442637836696115	39.0	34.0	40.0	16.0	40.0
35	34.430874109841575	39.0	34.0	40.0	16.0	40.0
36	34.43514827395825	39.0	34.0	40.0	16.0	40.0
37	34.40136336109919	39.0	34.0	40.0	16.0	40.0
38	34.41581076438324	39.0	34.0	40.0	16.0	40.0
39	34.3788909966165	39.0	34.0	40.0	16.0	40.0
40	34.3054588585385	39.0	34.0	40.0	16.0	40.0
41	34.27063957724243	39.0	34.0	40.0	16.0	40.0
42	34.272305929755184	39.0	34.0	40.0	16.0	40.0
43	34.18956213630002	39.0	34.0	40.0	16.0	40.0
44	34.05699186274549	39.0	34.0	40.0	16.0	40.0
45	34.06249390073539	39.0	34.0	40.0	16.0	40.0
46	34.02240151275131	39.0	34.0	40.0	16.0	40.0
47	33.949626424220064	39.0	31.0	40.0	16.0	40.0
48	33.970996910597144	39.0	31.0	40.0	16.0	40.0
49	33.92476816110347	39.0	31.0	40.0	16.0	40.0
50	33.823129347188186	39.0	31.0	40.0	16.0	40.0
51	33.8542279099675	39.0	31.0	40.0	16.0	40.0
52	33.87605960101117	39.0	31.0	40.0	16.0	40.0
53	33.82147368927639	39.0	31.0	40.0	16.0	40.0
54	33.84392366193832	39.0	31.0	40.0	16.0	40.0
55	33.82982015691653	39.0	31.0	40.0	16.0	40.0
56	33.76958415380975	39.0	31.0	40.0	16.0	40.0
57	33.652843226507635	38.0	31.0	40.0	16.0	40.0
58	33.67055415411722	38.0	31.0	40.0	16.0	40.0
59	33.60948664578546	38.0	31.0	40.0	16.0	40.0
60	33.56976188470953	38.0	31.0	40.0	16.0	40.0
61	33.53828232677086	38.0	31.0	40.0	16.0	40.0
62	33.48530695510006	38.0	31.0	40.0	16.0	40.0
63	33.445655050993196	38.0	31.0	40.0	16.0	40.0
64	33.42935246864811	38.0	31.0	40.0	16.0	40.0
65	33.3440316105668	38.0	31.0	40.0	16.0	40.0
66	33.29829247327353	38.0	31.0	39.0	16.0	40.0
67	33.282107865745324	38.0	31.0	39.0	16.0	40.0
68	33.23885288373231	38.0	31.0	39.0	16.0	40.0
69	33.19583117770283	38.0	31.0	39.0	16.0	40.0
70	33.086687093354506	38.0	31.0	39.0	16.0	40.0
71	32.980848309116745	38.0	30.0	39.0	16.0	40.0
72	32.94390414429156	38.0	30.0	39.0	16.0	40.0
73	32.88803922512269	38.0	30.0	39.0	16.0	40.0
74	32.83599862841743	38.0	30.0	39.0	16.0	40.0
75	32.72510631101771	38.0	30.0	39.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	9111.0
3	2393.0
4	2639.0
5	2574.0
6	2502.0
7	2579.0
8	2740.0
9	3105.0
10	3284.0
11	3671.0
12	4102.0
13	4598.0
14	5215.0
15	5909.0
16	6799.0
17	7611.0
18	9465.0
19	17873.0
20	33288.0
21	45436.0
22	51893.0
23	57193.0
24	61734.0
25	65127.0
26	69167.0
27	73535.0
28	77480.0
29	82231.0
30	88831.0
31	96698.0
32	104865.0
33	115781.0
34	130610.0
35	151905.0
36	188672.0
37	259623.0
38	452984.0
39	688941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.52514321236873	17.50228939259239	12.830625054310406	25.141942340728463
2	15.231183852222005	20.12931109391063	39.492320608095014	25.147184445772357
3	18.909558433294432	25.415518668094393	28.437746059373755	27.23717683923742
4	12.406706316899742	16.136080776321084	37.44126324626592	34.015949660513265
5	13.939042111328122	38.129895286488306	33.76435917282608	14.166703429357483
6	32.1963969889351	36.88664792437847	17.595158554143424	13.321796532543
7	28.674464367594826	31.899120502753192	21.489530654068425	17.936884475583557
8	27.048617655984096	34.79886129236232	19.712121394415547	18.44039965723804
9	27.214116605907968	15.750339887786899	18.50643881819312	38.529104688112014
10	14.327022181939226	29.483477509922583	33.4634732588187	22.726027049319487
11	35.555370628080546	23.08386171346223	23.4161630178025	17.944604640654724
12	24.08374006237626	24.64694448566322	29.871724945557798	21.397590506402725
13	28.148924992079312	22.053269807403606	25.651635405011223	24.146169795505863
14	22.48382775810417	20.58663896765017	26.853875656548237	30.075657617697427
15	23.667252196069466	28.936214726198163	23.31406299922063	24.08247007851174
16	23.87626480366718	28.050902290115115	24.594206734657593	23.478626171560116
17	22.834878034760127	27.824577797206302	26.213803788829757	23.126740379203813
18	22.95469098618926	27.5616911372505	26.745893607435956	22.737724269124286
19	23.95276462119055	26.96613554604627	26.672669011457927	22.40843082130525
20	24.144866391013327	26.473147862216106	26.55496155959366	22.827024187176907
21	24.21832493138745	26.324359226299094	26.51936859075906	22.937947251554395
22	23.908615971584446	26.21691190723503	26.482572479315973	23.39189964186455
23	23.635569440712477	26.408077899473426	26.484577716996792	23.471774942817305
24	23.302733406323988	26.36456424179958	26.602987002049357	23.72971534982708
25	23.598539384873288	26.265104452830794	26.54904610843523	23.587310053860683
26	23.44991785209634	26.553925520125233	26.55419288514934	23.441963742629078
27	23.544097181838964	26.700341291453277	26.274194863650525	23.481366663057237
28	23.71800254029465	26.509449835871006	26.36400341605069	23.408544207783656
29	23.801536279428532	26.629322456924154	26.25400880433024	23.31513245931707
30	23.707356949685913	26.60151649441675	26.255445891334833	23.435680664562504
31	23.67784653514981	26.23455799882627	26.41452808068007	23.673067385343852
32	23.4629853176497	26.577019174082707	26.345113436295602	23.61488207197199
33	23.477382767568145	26.54846454270658	26.311391541609826	23.662761148115454
34	23.878437144488068	26.481369336707477	26.317975886348478	23.322217632455974
35	23.862462084297515	26.52956188230324	26.281614243069562	23.326361790329674
36	23.852034848357242	26.49634177805762	26.169454615455567	23.482168758129568
37	23.780113656871748	26.220454493804485	26.42502215787637	23.574409691447393
38	23.791589478141514	26.35430978502627	26.301036752042773	23.553063984789446
39	23.87601837205517	26.610938678603667	26.114573686572847	23.398469262768316
40	23.833954288601827	26.358013798708896	26.480533821007135	23.32749809168214
41	23.57930514731071	26.378066570971658	26.42321812612691	23.61941015559072
42	23.675655659275417	26.481539472905858	26.19836431505066	23.644440552768067
43	23.547637826854178	26.10859713384311	26.62665098123095	23.71711405807176
44	23.63800368430481	26.378238806049957	26.3677112660052	23.616046243640028
45	23.700143509423697	26.55475783536722	26.15490996738787	23.590188687821218
46	23.81878928387257	26.30321275946531	26.152986986337307	23.72501097032481
47	23.71743241174776	26.522227071929926	26.159245388451563	23.60109512787075
48	23.550513942417595	26.665750941459095	26.13950973275529	23.644225383368024
49	23.714683992817235	26.308058752146856	26.169730726568037	23.807526528467868
50	23.830034546937814	26.544694620376376	26.154107349170047	23.47116348351576
51	23.78521889320835	26.539899149238806	25.965494976177617	23.70938698137523
52	23.92096417239441	26.414412053892804	25.88559710336539	23.779026670347395
53	23.810222461663095	26.49698077239288	26.128784729138456	23.56401203680557
54	23.62047010932231	26.501986918755243	26.19781622756152	23.679726744360927
55	23.510436419954026	26.37282353169227	26.268984044078476	23.847756004275233
56	23.54299827381937	26.6981489929499	26.102524768937442	23.656327964293293
57	23.74085067003458	26.457517799020707	26.0594440335557	23.742187497389008
58	23.751599185601783	26.348830338606245	26.230052791506303	23.669517684285672
59	23.7090097024171	26.56121486253489	26.05525608585959	23.674519349188426
60	23.582282698897522	26.66264716764018	26.281775125740246	23.47329500772205
61	23.86380992773262	26.388038032367415	26.224473051048385	23.52367898885158
62	23.770271277425945	26.531239064965344	26.13496259988376	23.56352705772495
63	23.60100730749114	26.518622989938624	26.186986883947416	23.693382818622823
64	23.594582969460774	26.46104650249539	26.173825305744856	23.77054522229898
65	23.670304585917226	26.46419983322722	26.066084131686996	23.79941144916856
66	23.569725983628803	26.646544682569072	26.13165272097976	23.652076612822366
67	23.729737461807733	26.45150660172227	26.06151269109501	23.757243245374983
68	23.705977880695055	26.484042530080576	26.373482769488582	23.436496819735787
69	23.6791374504522	26.469489248210266	26.07113493713361	23.780238364203925
70	23.475165690183587	26.17146218453692	26.426840639966848	23.926531485312648
71	23.633136335942105	26.282090059547063	26.21875633963852	23.866017264872312
72	23.517710538760657	26.56843167694079	26.122330301506107	23.791527482792446
73	23.6194041074144	26.350216399578898	26.125728990859415	23.904650502147284
74	23.50683666075778	26.510776363388437	26.062763861149215	23.91962311470457
75	23.589878474044138	26.43732633539185	26.25498058565872	23.71781460490529
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	196.0
1	271.0
2	420.5
3	501.0
4	507.0
5	476.5
6	442.0
7	465.0
8	492.0
9	610.5
10	856.0
11	1009.0
12	1035.0
13	1104.0
14	1228.0
15	1396.5
16	1510.0
17	1705.5
18	2218.5
19	2997.5
20	3459.0
21	4180.0
22	6587.5
23	9703.5
24	11133.0
25	13289.5
26	18708.5
27	24403.5
28	26836.0
29	31291.5
30	39498.5
31	47835.0
32	52420.0
33	56738.5
34	66248.5
35	76782.0
36	82124.0
37	86359.0
38	93448.0
39	100495.0
40	104688.0
41	109775.0
42	119295.5
43	127267.0
44	130805.0
45	135190.5
46	143077.5
47	149357.0
48	152135.0
49	154251.0
50	156430.0
51	155327.0
52	154161.0
53	151542.0
54	146826.0
55	139682.0
56	134635.0
57	129425.5
58	114807.0
59	94727.5
60	84057.0
61	76403.0
62	60158.0
63	44385.0
64	37203.0
65	30856.5
66	20562.5
67	14158.5
68	11702.0
69	9863.0
70	6530.5
71	3970.0
72	2903.0
73	2636.0
74	1973.5
75	1166.0
76	754.0
77	578.0
78	297.0
79	141.5
80	91.0
81	68.0
82	30.5
83	13.0
84	10.0
85	9.0
86	6.5
87	3.5
88	2.0
89	2.0
90	1.5
91	0.5
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.003475745313425334
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.091806465153648
29	0.0
30	0.0
31	0.0
32	0.0
33	0.050933037092886616
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0010360394684248591
39	3.6762690815075644E-4
40	0.0
41	6.349919322603975E-4
42	7.686744443152181E-4
43	1.336825120548205E-4
44	4.0104753616446154E-4
45	8.689363283563335E-4
46	3.342062801370513E-5
47	6.684125602741026E-5
48	0.0
49	3.342062801370513E-5
50	1.0026188404111539E-4
51	6.684125602741027E-4
52	6.684125602741026E-5
53	2.67365024109641E-4
54	0.003141539033288282
55	0.0015373488886304362
56	3.007856521233462E-4
57	1.6710314006852567E-4
58	5.34730048219282E-4
59	7.686744443152181E-4
60	0.002773912125137526
61	0.0027070708691101157
62	0.0020386583088360132
63	9.691982123974489E-4
64	0.0015039282606167307
65	0.002306023332945654
66	0.0026736502410964107
67	0.0022057614489045386
68	0.0039102134776035
69	0.003475745313425334
70	0.004478364153836487
71	0.0025733883570552953
72	6.349919322603975E-4
73	4.678887921918718E-4
74	0.002005237680822308
75	0.0015373488886304362
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	2992164.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.774582577154156
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.99058263348383	44.48098774021422
2	11.408148029300552	13.182019807424464
3	4.592505564116508	7.959902758502674
4	2.298693091325471	5.312241352972687
5	1.2985414119433776	3.7511344017088506
6	0.8100082053657972	2.8078731569447215
7	0.5572312526181713	2.253566211327171
8	0.3717503148765036	1.7182175411932492
9	0.2739976407916479	1.4247089391476235
>10	1.3273621781308453	13.074950815668018
>50	0.05289534933897118	2.057022418567116
>100	0.017635642860346433	1.7434467778047131
>500	6.48685848060292E-4	0.23392807852446548
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	3.342062801370513E-5
13	0.0	0.0	0.0	0.0	6.684125602741026E-5
14	0.0	0.0	0.0	3.342062801370513E-5	6.684125602741026E-5
15	0.0	0.0	0.0	3.342062801370513E-5	1.002618840411154E-4
16	0.0	0.0	0.0	3.342062801370513E-5	1.002618840411154E-4
17	0.0	0.0	0.0	3.342062801370513E-5	1.002618840411154E-4
18	0.0	0.0	0.0	3.342062801370513E-5	1.002618840411154E-4
19	0.0	0.0	0.0	3.342062801370513E-5	1.002618840411154E-4
20	0.0	0.0	0.0	3.342062801370513E-5	1.336825120548205E-4
21	0.0	0.0	0.0	3.342062801370513E-5	1.6710314006852567E-4
22	0.0	0.0	0.0	3.342062801370513E-5	1.6710314006852567E-4
23	0.0	0.0	0.0	1.6710314006852567E-4	1.6710314006852567E-4
24	0.0	0.0	0.0	3.3420628013705134E-4	1.6710314006852567E-4
25	0.0	3.342062801370513E-5	0.0	3.6762690815075644E-4	1.6710314006852567E-4
26	0.0	3.342062801370513E-5	0.0	4.010475361644616E-4	1.6710314006852567E-4
27	0.0	3.342062801370513E-5	0.0	4.010475361644616E-4	1.6710314006852567E-4
28	0.0	3.342062801370513E-5	0.0	7.352538163015129E-4	1.6710314006852567E-4
29	0.0	3.342062801370513E-5	0.0	0.0014705076326030258	1.6710314006852567E-4
30	0.0	3.342062801370513E-5	0.0	0.002306023332945654	1.6710314006852567E-4
31	0.0	3.342062801370513E-5	0.0	0.005614665506302462	1.6710314006852567E-4
32	0.0	3.342062801370513E-5	0.0	0.009458037727878552	1.6710314006852567E-4
33	0.0	3.342062801370513E-5	0.0	0.013067465553358706	1.6710314006852567E-4
34	0.0	3.342062801370513E-5	0.0	0.01701109965897591	1.6710314006852567E-4
35	0.0	3.342062801370513E-5	0.0	0.022358400141168733	1.6710314006852567E-4
36	0.0	6.684125602741026E-5	0.0	0.028908843231854938	1.6710314006852567E-4
37	0.0	6.684125602741026E-5	0.0	0.03796583342356903	2.005237680822308E-4
38	0.0	6.684125602741026E-5	0.0	0.04983015636843435	2.005237680822308E-4
39	0.0	6.684125602741026E-5	0.0	0.06453523269446461	2.005237680822308E-4
40	0.0	6.684125602741026E-5	0.0	0.07756927761980961	2.005237680822308E-4
41	0.0	6.684125602741026E-5	0.0	0.0918733064096754	2.005237680822308E-4
42	0.0	6.684125602741026E-5	0.0	0.10667864461974678	2.005237680822308E-4
43	0.0	6.684125602741026E-5	0.0	0.12335553799858565	2.005237680822308E-4
44	0.0	6.684125602741026E-5	0.0	0.1407008439376986	2.005237680822308E-4
45	0.0	6.684125602741026E-5	0.0	0.15704353103640042	2.005237680822308E-4
46	0.0	6.684125602741026E-5	0.0	0.17682854282051386	2.005237680822308E-4
47	0.0	6.684125602741026E-5	0.0	0.1966803958606547	2.005237680822308E-4
48	0.0	6.684125602741026E-5	0.0	0.21850406595360414	2.005237680822308E-4
49	0.0	6.684125602741026E-5	0.0	0.2395924822302521	2.005237680822308E-4
50	0.0	6.684125602741026E-5	0.0	0.26235192990758527	2.3394439609593593E-4
51	0.0	6.684125602741026E-5	0.0	0.2880523928501245	2.3394439609593593E-4
52	0.0	6.684125602741026E-5	0.0	0.31308444323238965	2.3394439609593593E-4
53	0.0	6.684125602741026E-5	0.0	0.33774886670650406	2.3394439609593593E-4
54	0.0	6.684125602741026E-5	0.0	0.36381695655719404	2.3394439609593593E-4
55	0.0	6.684125602741026E-5	0.0	0.3905534589681582	2.3394439609593593E-4
56	0.0	6.684125602741026E-5	0.0	0.42029781790035575	2.3394439609593593E-4
57	0.0	6.684125602741026E-5	0.0	0.45061032750878627	2.3394439609593593E-4
58	0.0	6.684125602741026E-5	0.0	0.4839975348944777	2.3394439609593593E-4
59	0.0	6.684125602741026E-5	0.0	0.5179528929564021	2.3394439609593593E-4
60	0.0	6.684125602741026E-5	0.0	0.5527771873466829	2.3394439609593593E-4
61	0.0	6.684125602741026E-5	0.0	0.5881362117851829	2.3394439609593593E-4
62	0.0	6.684125602741026E-5	0.0	0.6293104254980676	2.3394439609593593E-4
63	0.0	6.684125602741026E-5	0.0	0.6681451952499929	2.3394439609593593E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3735	0.0	41.00714	1
GGTATCA	1475	0.0	32.97571	1
TCAACGC	4890	0.0	29.838491	4
ATCAACG	4905	0.0	29.817566	3
TATCAAC	5080	0.0	29.537306	2
CAACGCA	5040	0.0	28.74512	5
AACGCAG	5300	0.0	27.269897	6
GTACATG	9600	0.0	22.889448	1
ACGCAGA	6230	0.0	22.534697	7
TACATGG	9720	0.0	21.64756	2
CGCAGAG	6500	0.0	21.492504	8
ACATGGG	9760	0.0	20.145145	3
AGAGTAC	6560	0.0	19.876198	11
CAGAGTA	7390	0.0	18.157269	10
CATGGGG	6165	0.0	18.128307	4
GCAGAGT	7500	0.0	17.844969	9
ATACCGT	295	2.910383E-11	17.539394	6
GAGTACT	2715	0.0	16.643583	12
GAGTACA	6560	0.0	15.775552	1
GGACCGT	350	2.1827873E-11	15.768751	6
>>END_MODULE