FastQCFastQC Report
Mon 6 Feb 2017
SRR3203959_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203959_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2992164
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61400.042.4017751
GGTATCA23300.037.2945671
TATCAAC84150.030.4718362
TCAACGC82950.030.3249664
ATCAACG84600.029.9869483
CAACGCA83950.029.8266325
AACGCAG87550.028.5611616
ACGCAGA102450.024.2967247
CGCAGAG106000.023.2257028
TCGTATG15150.022.40007840
AGAGTAC107250.022.2038911
GAGTACT62250.022.01847312
GTACATG103900.021.7318231
TACATGG103800.021.1789822
AGTACTT65000.020.9821913
TCTCGTA12450.020.86065738
CAGAGTA117200.020.65345610
ACATGGG104250.020.530383
CTCGTAT15350.020.5290639
GCAGAGT119150.020.4863389
Produced by FastQC (version 0.11.5)