##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3203959_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2992164 Sequences flagged as poor quality 0 Sequence length 75 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.583004140147395 35.0 32.0 35.0 27.0 35.0 2 32.57902775382633 35.0 32.0 35.0 27.0 35.0 3 32.59244379652987 35.0 32.0 35.0 27.0 35.0 4 32.71456511073591 35.0 32.0 35.0 30.0 35.0 5 32.79327302915215 35.0 32.0 35.0 30.0 35.0 6 36.28318033369829 39.0 36.0 40.0 27.0 40.0 7 36.08898175367393 39.0 36.0 40.0 27.0 40.0 8 35.93187338661918 39.0 36.0 40.0 27.0 40.0 9 35.91287208856199 39.0 36.0 40.0 27.0 40.0 10 35.845900826291604 39.0 36.0 40.0 27.0 40.0 11 35.924551929640224 39.0 36.0 40.0 27.0 40.0 12 35.93131192006855 39.0 36.0 40.0 27.0 40.0 13 35.92927092231576 39.0 36.0 40.0 27.0 40.0 14 35.873230879056095 39.0 36.0 40.0 27.0 40.0 15 35.85407450928492 39.0 36.0 40.0 27.0 40.0 16 35.845746088783905 39.0 36.0 40.0 27.0 40.0 17 35.58376813570379 39.0 35.0 40.0 27.0 40.0 18 35.54094327717331 39.0 35.0 40.0 27.0 40.0 19 35.724319589434266 39.0 35.0 40.0 27.0 40.0 20 35.70741744102262 39.0 35.0 40.0 27.0 40.0 21 35.507621574218525 39.0 35.0 40.0 27.0 40.0 22 35.520574741224074 39.0 35.0 40.0 27.0 40.0 23 35.31913023484007 39.0 35.0 40.0 27.0 40.0 24 35.37473447311043 39.0 35.0 40.0 27.0 40.0 25 35.15905311339886 39.0 35.0 40.0 16.0 40.0 26 35.20384711533191 39.0 35.0 40.0 16.0 40.0 27 35.299984893876136 39.0 35.0 40.0 27.0 40.0 28 35.28253297613366 39.0 35.0 40.0 27.0 40.0 29 35.239422371233665 39.0 35.0 40.0 16.0 40.0 30 35.17179472782909 39.0 34.0 40.0 16.0 40.0 31 35.203313053696256 39.0 34.0 40.0 16.0 40.0 32 34.98416564065339 39.0 34.0 40.0 16.0 40.0 33 35.209234520567726 39.0 34.0 40.0 27.0 40.0 34 35.22584724634078 39.0 35.0 40.0 16.0 40.0 35 35.27012456536473 39.0 35.0 40.0 27.0 40.0 36 35.25150860714854 39.0 35.0 40.0 16.0 40.0 37 35.06496970085864 39.0 34.0 40.0 16.0 40.0 38 35.101847024427805 39.0 34.0 40.0 16.0 40.0 39 35.1138908161451 39.0 34.0 40.0 16.0 40.0 40 35.170218276805684 39.0 35.0 40.0 16.0 40.0 41 35.283915587514585 39.0 35.0 40.0 27.0 40.0 42 35.0245300725495 39.0 34.0 40.0 16.0 40.0 43 35.01154281650337 39.0 34.0 40.0 16.0 40.0 44 35.0536451210562 39.0 34.0 40.0 16.0 40.0 45 34.863310299836506 39.0 34.0 40.0 16.0 40.0 46 34.792863626458974 39.0 34.0 40.0 16.0 40.0 47 34.86455822608654 39.0 34.0 40.0 16.0 40.0 48 34.75402985932589 39.0 34.0 40.0 16.0 40.0 49 34.760694600964385 39.0 34.0 40.0 16.0 40.0 50 34.67473240103149 39.0 34.0 40.0 16.0 40.0 51 34.40258054037145 39.0 34.0 40.0 16.0 40.0 52 34.65840609004052 39.0 34.0 40.0 16.0 40.0 53 34.659829474587625 39.0 34.0 40.0 16.0 40.0 54 34.59430365447883 39.0 34.0 40.0 16.0 40.0 55 34.519802390510684 39.0 34.0 40.0 16.0 40.0 56 34.544221840781454 39.0 34.0 40.0 16.0 40.0 57 34.488432118025614 39.0 34.0 40.0 16.0 40.0 58 34.529292846247735 39.0 34.0 40.0 16.0 40.0 59 34.48943741051627 39.0 34.0 40.0 16.0 40.0 60 34.22218100344767 39.0 34.0 40.0 16.0 40.0 61 34.423002215119226 39.0 34.0 40.0 16.0 40.0 62 34.15962494034418 39.0 34.0 40.0 16.0 40.0 63 34.31449078325921 39.0 34.0 40.0 16.0 40.0 64 34.283718071603026 39.0 34.0 40.0 16.0 40.0 65 34.24841519381959 39.0 34.0 40.0 16.0 40.0 66 34.25080811078537 39.0 34.0 40.0 16.0 40.0 67 34.22609723263832 39.0 34.0 40.0 16.0 40.0 68 34.14532492202967 39.0 34.0 40.0 16.0 40.0 69 34.03578346641427 39.0 34.0 40.0 16.0 40.0 70 33.95218577591335 39.0 34.0 40.0 16.0 40.0 71 33.90965668994079 39.0 34.0 40.0 16.0 40.0 72 33.90934053079978 39.0 34.0 40.0 16.0 40.0 73 33.75496162643491 39.0 34.0 40.0 16.0 40.0 74 33.67162428262622 39.0 34.0 40.0 16.0 40.0 75 33.61029408815827 39.0 34.0 40.0 16.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 888.0 3 1651.0 4 1507.0 5 1440.0 6 1836.0 7 2453.0 8 3682.0 9 5380.0 10 5132.0 11 3979.0 12 4298.0 13 4834.0 14 3103.0 15 3600.0 16 4378.0 17 4988.0 18 5954.0 19 8249.0 20 15024.0 21 24668.0 22 33923.0 23 42495.0 24 50951.0 25 57682.0 26 61632.0 27 67387.0 28 73471.0 29 79125.0 30 85881.0 31 93137.0 32 101078.0 33 111312.0 34 123133.0 35 141267.0 36 173137.0 37 232664.0 38 397551.0 39 959294.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.95632514705794 18.030276575291055 12.98384620479135 25.029552072859655 2 15.4903942430963 19.957261700896073 39.317129676047166 25.235214379960457 3 18.55944166302783 25.89206127155802 28.63971229891383 26.908784766500315 4 12.457798524967123 16.638540709188746 37.046583874815134 33.857076891028996 5 13.85404032588777 38.143487809288075 33.73968482720843 14.262787037615727 6 31.96451886314586 37.33737672963019 17.082234856906904 13.615869550317047 7 28.38684644290888 32.08072819537966 21.392109523408475 18.140315838302982 8 26.70192226698346 34.83816429319218 19.593398482990807 18.866514956833562 9 27.0276548214121 15.33000359622303 18.421217983254166 39.221123599110705 10 14.15715459256551 29.40415659312564 33.35123566170548 23.08745315260337 11 35.4171589258184 22.812305637705506 23.46314047023602 18.30739496624007 12 24.142592687285415 24.397394365420496 30.033678338281312 21.426334609012773 13 28.26409396376898 21.638450442694374 26.039292384159523 24.058163209377128 14 22.501911708479163 20.277010569207857 27.0691236354011 30.151954086911882 15 23.516955171343135 28.528022882069976 23.811570511995676 24.143451434591217 16 23.75845942816806 27.75257292287782 24.799393568294427 23.689574080659696 17 22.441961453556285 27.47909762889642 26.421981592603334 23.65695932494396 18 22.745222135661447 26.77316994353585 27.47746328355567 23.00414463724703 19 23.704331658469517 26.398008749494817 27.62211569029048 22.275543901745184 20 23.802604379765047 25.87321866467708 27.48935678050633 22.834820175051547 21 23.93845316287572 25.756806252973067 27.366780544205238 22.937960039945974 22 23.494095458266592 25.86385728528528 27.45861658125927 23.183430675188855 23 23.4106852657816 25.7855150335908 27.32601721644422 23.477782484183383 24 23.12440577056728 25.954258847602972 27.46030757736131 23.461027804468436 25 23.308867193995106 25.817477849830162 27.43852076567818 23.43513419049655 26 23.212640925658352 26.0677239698247 27.479925381924865 23.23970972259208 27 23.30202062520033 26.262161887970585 27.23268619354604 23.203131293283043 28 23.311298064834514 26.0518158478259 27.247909175088857 23.38897691225073 29 23.470119308397564 26.00553651365472 27.193362385480352 23.330981792467362 30 23.50024959745879 25.918260792772763 27.27458113636448 23.30690847340397 31 23.24120708307924 25.82707612610453 27.378121493469116 23.553595297347112 32 23.248541681925158 26.014679878805104 27.35832722149863 23.37845121777111 33 23.391180407657362 25.89194582806389 27.289531233353575 23.42734253092517 34 23.427812223206377 26.03980531162143 27.25503198653086 23.27735047864134 35 23.40546500405462 25.973569711469963 27.47939418380787 23.141571100667548 36 23.63073773007066 25.946635679220588 27.09655903115223 23.326067559556527 37 23.394238885347065 25.836457134872965 27.363522750398833 23.405781229381137 38 23.37102365835228 25.834735991339357 27.27488434390995 23.51935600639841 39 23.635038929754376 26.168094270228416 26.873057545059574 23.323809254957627 40 23.491795151271262 25.815859123780328 27.564274320233956 23.128071404714458 41 23.350975267436368 25.89901626859183 27.11886734961701 23.631141114354794 42 23.552932556409704 25.917541346108152 27.042516784981363 23.487009312500778 43 23.400001210146677 25.56771005961317 27.461152610925073 23.571136119315085 44 23.322656566548662 25.99129768223596 27.085626950487534 23.600418800727844 45 23.41063430503366 26.006348848831234 26.9980925298966 23.5849243162385 46 23.83561625469955 25.773856547828732 26.69112538130155 23.69940181617017 47 23.434214711656324 26.037658820883465 26.81215939420896 23.715967073251257 48 23.336030336933288 26.158264729561147 26.682035341696054 23.82366959180951 49 23.626776520980027 25.880957887545936 26.66125015206792 23.831015439406116 50 23.657364392901307 26.16907857357709 26.861000635005517 23.312556398516094 51 23.527531264752906 26.13059841583583 26.380162228305775 23.96170809110549 52 23.639155272108617 26.124183365799837 26.555945391924084 23.680715970167462 53 23.628679821008358 26.203419154626133 26.702781837932193 23.465119186433316 54 23.370718277597792 26.212966147084988 26.533087720215555 23.88322785510167 55 23.324338674122263 26.109333835152427 26.84542851766224 23.72089897306307 56 23.43680337528149 26.364674246166413 26.59167155174315 23.60685082680894 57 23.533457774004823 26.259677713143642 26.348178760082952 23.858685752768583 58 23.48935026333157 26.237966737735295 26.694175735151422 23.578507263781713 59 23.368595973829088 26.41967444111411 26.602928318756803 23.608801266300002 60 23.46067552100278 26.626086634043062 26.548713738830237 23.36452410612392 61 23.59716957358447 26.42950103239977 26.58093526261857 23.392394131397186 62 23.581393051442657 26.53871497017376 26.47994621096069 23.399945767422885 63 23.405779906353082 26.453816563004455 26.59248217800935 23.547921352633107 64 23.388206603963933 26.419006288372245 26.572579050422945 23.62020805724088 65 23.445951803488228 26.425254009823707 26.497463429910496 23.631330756777572 66 23.315704206356713 26.597609689591216 26.479487965388397 23.607198138663673 67 23.448110577636587 26.359357263476248 26.523210152862514 23.66932200602465 68 23.574496900920366 26.34194292773449 26.713284329192316 23.370275842152832 69 23.471306973499715 26.39104118292427 26.377800594049916 23.759851249526104 70 23.292141711188613 26.095596107922674 26.65224086143498 23.960021319453734 71 23.4389881156338 26.147076511436584 26.600107173576067 23.813828199353548 72 23.385156951320706 26.328613876460178 26.493532158392803 23.792697013826317 73 23.507236058115765 26.173053685049712 26.4695824830401 23.850127773794423 74 23.423775993711384 26.352148278636605 26.343181878703486 23.88089384894852 75 23.52614024872925 26.213020779162576 26.502570599088543 23.75826837301963 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 127.0 1 131.5 2 267.0 3 444.5 4 491.0 5 408.0 6 391.0 7 629.0 8 801.0 9 1468.5 10 2871.0 11 3401.0 12 3196.0 13 3112.0 14 3188.5 15 3604.0 16 3859.0 17 4119.5 18 4662.0 19 5456.5 20 5969.0 21 6843.5 22 9187.0 23 12012.0 24 13368.0 25 15425.0 26 20654.5 27 25999.5 28 28172.0 29 32326.5 30 40216.0 31 48167.0 32 52383.0 33 56480.5 34 65764.0 35 76324.0 36 81698.0 37 85255.0 38 91617.5 39 98789.0 40 103155.0 41 108309.5 42 117857.0 43 126249.5 44 130249.0 45 135366.0 46 144415.5 47 150185.5 48 152023.0 49 153544.5 50 155185.0 51 153405.0 52 151506.0 53 149423.5 54 145051.5 55 137575.0 56 132388.0 57 127239.0 58 112918.5 59 93076.5 60 82406.0 61 74759.0 62 58269.0 63 42544.5 64 35663.0 65 29421.5 66 19392.5 67 13334.0 68 11063.0 69 9310.0 70 6061.5 71 3592.5 72 2619.0 73 2416.5 74 1857.5 75 1087.0 76 673.0 77 520.5 78 269.0 79 127.5 80 85.0 81 70.0 82 34.0 83 10.0 84 7.0 85 5.0 86 1.5 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.08442050636261916 2 0.0 3 0.04284524511356998 4 0.04849333124788615 5 0.003041277149247167 6 0.04274498322952886 7 0.0 8 0.016309266470688106 9 0.004545205409863898 10 6.684125602741026E-5 11 2.339443960959359E-4 12 0.0011028807244522693 13 0.001069460096438564 14 0.00253996772904159 15 0.004244419757740552 16 0.007887268211234411 17 0.06527048651076613 18 0.3018551122197847 19 0.022625765165278373 20 0.019651329272058617 21 0.3052974369051964 22 0.3076703014941694 23 0.9791909801735466 24 0.6977892922981495 25 1.511748687571938 26 0.9806614878061497 27 0.8425674528535201 28 0.31315128448841706 29 0.8460431981669453 30 0.8484494833839321 31 0.3120484037639648 32 0.9803272815260127 33 0.557288972128533 34 0.31234918941608814 35 0.30569848444136083 36 0.8910607841014061 37 0.9745789335076552 38 0.8414645721290679 39 0.5530445523707925 40 0.8380890886996836 41 0.2984796287904005 42 0.8380890886996836 43 0.5787784359413455 44 0.855501235894824 45 0.288453440386289 46 0.28852028164231636 47 0.2905255193231387 48 0.2876179246859463 49 0.0026736502410964107 50 0.002138920192877128 51 1.102914145080283 52 0.2866153058455352 53 0.2861139964253296 54 0.2877516071980012 55 0.5455583316957225 56 0.0019383964247948978 57 0.003642848453493859 58 0.28952290048272755 59 0.004678887921918718 60 0.8692371140084568 61 0.0035425865694527436 62 0.5378381666245567 63 0.00247312647301418 64 0.28614741705334334 65 0.5380052697646253 66 0.01119591038459122 67 0.01523980637424954 68 0.010293553428221181 69 0.29787805748615387 70 0.5507051084098331 71 0.02523257415034737 72 0.5598289398575746 73 0.018347924779524117 74 0.8533288950739331 75 0.06700835916747878 >>END_MODULE >>Sequence Length Distribution pass #Length Count 75 2992164.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.86902561403755 #Duplication Level Percentage of deduplicated Percentage of total 1 74.29282442992033 40.76374886584493 2 12.74276516502299 13.983662164666235 3 5.08910249233701 8.37702285013505 4 2.561562861996792 5.622018331474772 5 1.453966116135915 3.9888852034102107 6 0.9090887815486269 2.992848938413548 7 0.6252356993119259 2.401425152024972 8 0.4479351535174345 1.9662212329420763 9 0.31721602602093724 1.5664800831233405 >10 1.4762694354870072 13.816573453128331 >50 0.064180070924106 2.3751317149782585 >100 0.018985103296184698 1.8062485683043559 >500 7.4456955502867E-4 0.2505019203572305 >1k 1.240949257756265E-4 0.08923152119681449 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 3.342062801370513E-5 0.0 0.0 0.0 9 0.0 3.342062801370513E-5 0.0 0.0 0.0 10 0.0 3.342062801370513E-5 0.0 0.0 0.0 11 0.0 3.342062801370513E-5 0.0 0.0 0.0 12 0.0 3.342062801370513E-5 0.0 0.0 0.0 13 0.0 3.342062801370513E-5 0.0 0.0 3.342062801370513E-5 14 0.0 3.342062801370513E-5 0.0 0.0 3.342062801370513E-5 15 0.0 3.342062801370513E-5 0.0 0.0 3.342062801370513E-5 16 0.0 3.342062801370513E-5 0.0 0.0 3.342062801370513E-5 17 0.0 3.342062801370513E-5 0.0 0.0 3.342062801370513E-5 18 0.0 3.342062801370513E-5 0.0 0.0 3.342062801370513E-5 19 0.0 3.342062801370513E-5 0.0 0.0 3.342062801370513E-5 20 0.0 3.342062801370513E-5 0.0 0.0 3.342062801370513E-5 21 0.0 3.342062801370513E-5 0.0 0.0 3.342062801370513E-5 22 0.0 3.342062801370513E-5 0.0 0.0 3.342062801370513E-5 23 0.0 3.342062801370513E-5 0.0 1.336825120548205E-4 3.342062801370513E-5 24 0.0 3.342062801370513E-5 0.0 2.67365024109641E-4 3.342062801370513E-5 25 0.0 3.342062801370513E-5 0.0 3.007856521233462E-4 3.342062801370513E-5 26 0.0 3.342062801370513E-5 0.0 3.3420628013705134E-4 3.342062801370513E-5 27 0.0 3.342062801370513E-5 0.0 3.3420628013705134E-4 3.342062801370513E-5 28 0.0 3.342062801370513E-5 0.0 6.349919322603975E-4 3.342062801370513E-5 29 0.0 3.342062801370513E-5 0.0 0.0013368251205482054 3.342062801370513E-5 30 0.0 3.342062801370513E-5 0.0 0.0021723408208908336 3.342062801370513E-5 31 0.0 3.342062801370513E-5 0.0 0.005414141738220231 3.342062801370513E-5 32 0.0 3.342062801370513E-5 0.0 0.009357775843837436 3.342062801370513E-5 33 0.0 6.684125602741026E-5 0.0 0.012800100529249065 3.342062801370513E-5 34 0.0 6.684125602741026E-5 0.0 0.016576631494797746 3.342062801370513E-5 35 0.0 6.684125602741026E-5 0.0 0.022057614489045387 3.342062801370513E-5 36 0.0 6.684125602741026E-5 0.0 0.02814016878753972 3.342062801370513E-5 37 0.0 1.002618840411154E-4 0.0 0.036896373327130465 3.342062801370513E-5 38 0.0 1.002618840411154E-4 0.0 0.0481925455957628 3.342062801370513E-5 39 0.0 1.002618840411154E-4 0.0 0.06246315375761489 3.342062801370513E-5 40 0.0 1.002618840411154E-4 0.0 0.07496246863474061 3.342062801370513E-5 41 0.0 1.002618840411154E-4 0.0 0.08866492612035971 3.342062801370513E-5 42 0.0 1.002618840411154E-4 0.0 0.10296895491022551 3.342062801370513E-5 43 0.0 1.002618840411154E-4 0.0 0.11877691196070804 3.342062801370513E-5 44 0.0 1.002618840411154E-4 0.0 0.1353535434555058 3.342062801370513E-5 45 0.0 1.002618840411154E-4 0.0 0.15089413548187866 3.342062801370513E-5 46 0.0 1.002618840411154E-4 0.0 0.1700441553337317 3.342062801370513E-5 47 3.342062801370513E-5 1.002618840411154E-4 0.0 0.18862602450935176 3.342062801370513E-5 48 3.342062801370513E-5 1.002618840411154E-4 0.0 0.20924655199380782 6.684125602741026E-5 49 3.342062801370513E-5 1.002618840411154E-4 0.0 0.2291986669179898 6.684125602741026E-5 50 3.342062801370513E-5 1.002618840411154E-4 0.0 0.2505878688467611 6.684125602741026E-5 51 6.684125602741026E-5 1.002618840411154E-4 0.0 0.2746841416446425 6.684125602741026E-5 52 6.684125602741026E-5 1.002618840411154E-4 0.0 0.2982122637662909 6.684125602741026E-5 53 6.684125602741026E-5 1.002618840411154E-4 0.0 0.3218406477719804 1.002618840411154E-4 54 6.684125602741026E-5 1.002618840411154E-4 0.0 0.345803238057807 1.002618840411154E-4 55 6.684125602741026E-5 1.002618840411154E-4 0.0 0.37096897095212694 1.002618840411154E-4 56 6.684125602741026E-5 1.002618840411154E-4 0.0 0.39844072717939255 1.002618840411154E-4 57 6.684125602741026E-5 1.002618840411154E-4 0.0 0.42688168161905565 1.002618840411154E-4 58 6.684125602741026E-5 1.002618840411154E-4 0.0 0.45809654818385626 1.002618840411154E-4 59 6.684125602741026E-5 1.002618840411154E-4 0.0 0.4896790416568076 1.002618840411154E-4 60 6.684125602741026E-5 1.002618840411154E-4 0.0 0.5229325665304442 1.002618840411154E-4 61 6.684125602741026E-5 1.002618840411154E-4 0.0 0.556954765848396 1.002618840411154E-4 62 6.684125602741026E-5 1.002618840411154E-4 0.0 0.5957895356003213 1.002618840411154E-4 63 6.684125602741026E-5 1.002618840411154E-4 0.0 0.6326190676714244 1.002618840411154E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6140 0.0 42.401775 1 GGTATCA 2330 0.0 37.294567 1 TATCAAC 8415 0.0 30.471836 2 TCAACGC 8295 0.0 30.324966 4 ATCAACG 8460 0.0 29.986948 3 CAACGCA 8395 0.0 29.826632 5 AACGCAG 8755 0.0 28.561161 6 ACGCAGA 10245 0.0 24.296724 7 CGCAGAG 10600 0.0 23.225702 8 TCGTATG 1515 0.0 22.400078 40 AGAGTAC 10725 0.0 22.20389 11 GAGTACT 6225 0.0 22.018473 12 GTACATG 10390 0.0 21.731823 1 TACATGG 10380 0.0 21.178982 2 AGTACTT 6500 0.0 20.98219 13 TCTCGTA 1245 0.0 20.860657 38 CAGAGTA 11720 0.0 20.653456 10 ACATGGG 10425 0.0 20.53038 3 CTCGTAT 1535 0.0 20.52906 39 GCAGAGT 11915 0.0 20.486338 9 >>END_MODULE