FastQCFastQC Report
Mon 6 Feb 2017
SRR3203958_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203958_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3658242
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA41500.035.1612431
CAACGCA49550.027.9857235
TCAACGC49600.027.9575124
ATCAACG49800.027.8452323
TATCAAC52550.026.8475532
AACGCAG52000.026.0701396
GGTATCA19600.026.0481911
ACGCAGA60050.022.4029797
GTACATG89450.020.9792771
CGCAGAG64250.020.777448
TACATGG88900.020.5649782
TATACCG3100.020.0292635
AGAGTAC63400.019.36934311
ACATGGG92100.018.9141083
ATACCGA1552.2511056E-517.803796
CGTATAC1753.2162625E-617.7402043
CATGGGG68500.017.5747434
CAGAGTA72900.017.0344910
GAGTACT30850.016.66039512
CGTATCG1250.001943290216.55797635
Produced by FastQC (version 0.11.5)