FastQCFastQC Report
Mon 6 Feb 2017
SRR3203958_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203958_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3658242
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA62900.040.696511
GGTATCA24650.035.2162251
TCAACGC80300.030.5723694
ATCAACG81200.030.5387233
CAACGCA80950.030.2714485
TATCAAC83600.029.8662992
AACGCAG84250.029.0460266
ACGCAGA99000.024.2914737
CGCAGAG103400.022.9608868
AGAGTAC103350.021.5285711
GAGTACT70050.020.92276412
CAGAGTA110550.020.45564510
CTCGTAT16800.020.42216939
GTACATG90850.020.1634981
GACGTAG17250.020.09702126
GCAGAGT117600.019.6928679
CGAATCG3050.019.45170237
AGTACTT76700.019.42312213
TACATGG91250.019.3895242
TCTCGTA14900.019.30517838
Produced by FastQC (version 0.11.5)