FastQCFastQC Report
Mon 6 Feb 2017
SRR3203957_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203957_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3095954
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34050.038.0925371
TCAACGC43000.029.119664
ATCAACG43300.028.9975723
CAACGCA44600.028.075015
TATCAAC45350.027.838892
AACGCAG47000.027.0817456
GGTATCA17000.025.3647331
GTACATG77500.023.0567041
ACGCAGA54250.022.8266857
TACATGG79600.021.4506422
CGCAGAG56900.021.3392188
ATCGCGC850.00412409320.29211269
AGAGTAC55850.020.1963411
ACATGGG83150.019.5392023
GTATAGC9100.018.574791
CAGAGTA64000.018.05564510
ATAATAC10950.017.6409533
GCAGAGT66550.017.4674689
GTATAGA9200.017.2480181
CCGGTAT2401.2341843E-817.24773858
Produced by FastQC (version 0.11.5)