FastQCFastQC Report
Mon 6 Feb 2017
SRR3203957_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203957_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3095954
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55450.042.242461
GGTATCA23300.033.9984131
ATCAACG74150.031.3422833
TCAACGC73800.031.1043834
CAACGCA73700.030.8559325
TATCAAC74600.030.83152
AACGCAG76350.029.6966176
GACCGTA15150.026.77370626
CGTACTA15500.026.1678529
ACGCAGA88450.025.6602127
ACCGTAC16050.025.2723827
CGCAGAG90700.024.6479268
TATGCCG17250.023.775243
CCGTACT17150.023.65141128
AGAGTAC91450.023.2958411
CTCGTAT17000.023.2532739
TCGTATG18550.022.61805740
GAGTACT63450.022.5475212
TCTCGTA14200.022.46683538
GTACTAG18350.021.93345830
Produced by FastQC (version 0.11.5)