FastQCFastQC Report
Mon 6 Feb 2017
SRR3203956_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203956_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113724
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13000.042.9885331
GTATCAA34200.040.952541
GACGCGT400.002599135334.49232520
ATCAACG46400.029.6604253
TATCAAC47650.029.3166662
TCAACGC46800.029.1858124
CAACGCA48200.028.4096535
AACGCAG49650.027.4410236
ACGCAGA56400.023.912237
GTACATG72850.023.8187711
CGCAGAG55850.023.6536438
GAGTACT23350.023.0441212
ACACCGT902.1295034E-422.99488334
AACCGTT1653.1523086E-922.9948834
TACATGG75500.022.5228022
ACGAAAT1554.0958184E-822.2531112
CGTATAG1103.2690084E-521.9526161
TCGCTAA1103.2724092E-521.9496646
GTACTTT24200.021.80712914
AGTACTT25850.021.6160813
Produced by FastQC (version 0.11.5)