##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3203956_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1113724
Sequences flagged as poor quality	0
Sequence length	75
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.95775703854815	35.0	31.0	35.0	15.0	35.0
2	31.218917792918173	35.0	31.0	35.0	15.0	35.0
3	31.346371273313675	35.0	31.0	35.0	15.0	35.0
4	31.331410654704396	35.0	31.0	35.0	15.0	35.0
5	31.656416670557515	35.0	31.0	35.0	15.0	35.0
6	35.05690727684777	39.0	34.0	40.0	16.0	40.0
7	34.81394223344383	39.0	34.0	40.0	16.0	40.0
8	34.83818612151664	39.0	34.0	40.0	16.0	40.0
9	34.62978529689582	39.0	34.0	40.0	16.0	40.0
10	34.84873451591238	39.0	34.0	40.0	16.0	40.0
11	34.85455552722218	39.0	34.0	40.0	16.0	40.0
12	34.48161124300096	39.0	34.0	40.0	16.0	40.0
13	34.50292711659262	39.0	34.0	40.0	16.0	40.0
14	34.49650541785936	39.0	34.0	40.0	16.0	40.0
15	34.62630508097159	39.0	34.0	40.0	16.0	40.0
16	34.8058917649256	39.0	34.0	40.0	16.0	40.0
17	34.876868057076976	39.0	34.0	40.0	16.0	40.0
18	34.950345866659966	39.0	34.0	40.0	16.0	40.0
19	35.00806663051169	39.0	34.0	40.0	16.0	40.0
20	34.98238073346718	39.0	34.0	40.0	16.0	40.0
21	35.045153916051014	39.0	34.0	40.0	16.0	40.0
22	35.137086926383915	39.0	34.0	40.0	16.0	40.0
23	35.201801343959545	39.0	34.0	40.0	16.0	40.0
24	35.30166270997123	39.0	35.0	40.0	16.0	40.0
25	35.341794735499995	39.0	35.0	40.0	16.0	40.0
26	35.395822483846985	39.0	35.0	40.0	27.0	40.0
27	35.43423146129562	39.0	35.0	40.0	27.0	40.0
28	35.31982430117336	39.0	35.0	40.0	16.0	40.0
29	35.43256677596963	39.0	35.0	40.0	27.0	40.0
30	35.444378499520525	39.0	35.0	40.0	27.0	40.0
31	35.450211183381164	39.0	35.0	40.0	27.0	40.0
32	35.39071888546893	39.0	35.0	40.0	27.0	40.0
33	35.27481853672903	39.0	35.0	40.0	16.0	40.0
34	35.310077721230755	39.0	35.0	40.0	16.0	40.0
35	35.30146607238418	39.0	35.0	40.0	16.0	40.0
36	35.30256957738183	39.0	35.0	40.0	16.0	40.0
37	35.26153337810804	39.0	35.0	40.0	16.0	40.0
38	35.249136231238616	39.0	35.0	40.0	16.0	40.0
39	35.193635047821545	39.0	35.0	40.0	16.0	40.0
40	35.126465803017624	39.0	35.0	40.0	16.0	40.0
41	35.07028581587539	39.0	34.0	40.0	16.0	40.0
42	35.056728597031224	39.0	34.0	40.0	16.0	40.0
43	34.99526363802881	39.0	34.0	40.0	16.0	40.0
44	34.871744705151364	39.0	34.0	40.0	16.0	40.0
45	34.86450502997152	39.0	34.0	40.0	16.0	40.0
46	34.80826578218661	39.0	34.0	40.0	16.0	40.0
47	34.710237904543675	39.0	34.0	40.0	16.0	40.0
48	34.715933211459934	39.0	34.0	40.0	16.0	40.0
49	34.66362132808487	39.0	34.0	40.0	16.0	40.0
50	34.559106205846334	39.0	34.0	40.0	16.0	40.0
51	34.59613423074298	39.0	34.0	40.0	16.0	40.0
52	34.64069464247875	39.0	34.0	40.0	16.0	40.0
53	34.56818834828018	39.0	34.0	40.0	16.0	40.0
54	34.61633402889765	39.0	34.0	40.0	16.0	40.0
55	34.607875021100384	39.0	34.0	40.0	16.0	40.0
56	34.56922271586138	39.0	34.0	40.0	16.0	40.0
57	34.44454281312066	39.0	34.0	40.0	16.0	40.0
58	34.48291138558566	39.0	34.0	40.0	16.0	40.0
59	34.42596819319688	39.0	34.0	40.0	16.0	40.0
60	34.400377472336054	39.0	34.0	40.0	16.0	40.0
61	34.370651076927494	39.0	34.0	40.0	16.0	40.0
62	34.336894957817194	39.0	34.0	40.0	16.0	40.0
63	34.2895026056725	39.0	34.0	40.0	16.0	40.0
64	34.28795733952038	39.0	34.0	40.0	16.0	40.0
65	34.19957278463964	39.0	34.0	40.0	16.0	40.0
66	34.16024975667221	39.0	34.0	40.0	16.0	40.0
67	34.14395756937984	39.0	34.0	40.0	16.0	40.0
68	34.11426080429263	39.0	34.0	40.0	16.0	40.0
69	34.080474156972464	39.0	34.0	40.0	16.0	40.0
70	33.95412597735166	39.0	34.0	40.0	16.0	40.0
71	33.848888054850214	39.0	34.0	40.0	16.0	40.0
72	33.82588415083091	39.0	34.0	40.0	16.0	40.0
73	33.77471258588304	39.0	34.0	40.0	16.0	40.0
74	33.748104557322996	39.0	34.0	40.0	16.0	40.0
75	33.65541193329766	39.0	34.0	40.0	16.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5854.0
3	900.0
4	953.0
5	941.0
6	851.0
7	912.0
8	1098.0
9	1069.0
10	1120.0
11	1155.0
12	1296.0
13	1476.0
14	1740.0
15	1885.0
16	2221.0
17	2497.0
18	3066.0
19	5593.0
20	9719.0
21	12380.0
22	13575.0
23	15445.0
24	17229.0
25	18867.0
26	20603.0
27	22524.0
28	25254.0
29	27540.0
30	30976.0
31	34952.0
32	38565.0
33	42441.0
34	46714.0
35	53135.0
36	66654.0
37	93377.0
38	167282.0
39	321865.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.72744504875327	20.117916544323315	13.624245624148648	22.530392782774776
2	14.19759294044126	22.178295520254572	41.06951093807801	22.554600601226156
3	17.07613376384095	30.25632921621515	28.78747337760522	23.880063642338676
4	11.085690889304711	18.25111068810585	39.64528015917767	31.017918263411758
5	11.73692943673657	41.73906641142689	33.77596244671032	12.748041705126226
6	27.40274969382001	41.57574048866685	18.557200886395552	12.464308931117584
7	26.652743408600337	35.24643448466586	22.476214932963643	15.62460717377016
8	25.637590641846636	36.50563335260801	20.34058707543341	17.516188930111948
9	27.22945720842866	17.87166299729556	19.137147084915114	35.761732709360665
10	13.291264262959226	32.81998053377677	32.89935387941716	20.98940132384684
11	32.10562042301324	26.885296536664384	24.02157087393286	16.987512166389518
12	22.493274814945174	28.676853511282868	29.862515308999356	18.9673563647726
13	26.55945279081711	25.407372023948483	26.31028872503421	21.722886460200193
14	21.65725080899756	23.700036993007245	28.03019419533026	26.61251800266493
15	21.888187737715985	31.82081018277419	24.671283010871633	21.61971906863819
16	21.437537486845933	31.203422032747792	25.779187662293353	21.579852818112926
17	20.226106288452076	31.151883231393057	26.839504221871845	21.78250625828302
18	20.371564229557773	30.231637281768194	28.902492897701766	20.49430559097227
19	22.16491698122695	29.21379084943846	28.937690127895245	19.68360204143935
20	22.736602605313344	28.366453448071518	28.419967604182006	20.476976342433133
21	23.049606545248196	28.458576810771792	27.93088772442724	20.56092891955278
22	22.123614109061133	28.591105157112533	28.354780897242044	20.93049983658429
23	21.699361780836185	28.882380194734065	28.386745728744284	21.031512295685467
24	21.345593701850728	28.588860435799173	28.800133605812572	21.265412256537527
25	21.789330211075633	28.35181786510841	28.52322478459654	21.33562713921941
26	21.25544569390621	28.67918802144876	28.68511408571603	21.380252198928996
27	21.26711824473568	29.07955651489956	28.21632648663403	21.436998753730727
28	21.258976317799846	28.495933132611334	28.69249090010336	21.55259964948546
29	21.36453914973548	28.914614392793908	28.644888679780628	21.075957777689986
30	21.655544820799406	28.818091376319448	28.289773768007155	21.236590034873988
31	21.73635478808035	28.24927899551415	28.830212871411586	21.184153344993913
32	21.30626618444067	28.929698920019682	28.199087026947428	21.564947868592217
33	21.117871241673868	28.710709735845803	28.441901708979373	21.729517313500953
34	21.852471395490618	28.339632026994146	28.65631759423124	21.151578983283994
35	21.64369269226487	28.68349788637041	28.41978802647694	21.25302139488778
36	21.53711332430656	28.617054135494968	28.15087041313647	21.694962127062002
37	21.694802028870733	28.26411953421093	28.568593806538967	21.47248463037937
38	21.519489708327797	28.54892988876877	28.480959239159713	21.45062116374372
39	21.86373427456248	28.72469855556284	28.10982283713785	21.301744332736835
40	21.782596047135556	28.28447622570763	28.88408618293222	21.0488415442246
41	21.28208627693236	28.4716211314195	28.741707939905687	21.504584651742455
42	21.579405769904195	28.60511264153146	28.09492596816047	21.720555620403875
43	20.99371118876849	28.060453038634346	28.89360380130086	22.052231971296298
44	21.02930995935233	28.6922979536094	28.402996452420137	21.87539563461813
45	21.388781639744636	28.478239398047968	28.40874195257293	21.724237009634464
46	21.41347407436672	28.260682179786016	28.330358329352695	21.99548541649457
47	21.436549809468055	28.89153865769257	28.15850246560189	21.513409067237486
48	21.133262040920407	29.14863031471919	27.91690572970119	21.80120191465921
49	21.490872065251352	28.68349788637041	27.774026599049673	22.051603449328557
50	21.697226330065913	28.611961861252755	27.86563624886978	21.82517555981155
51	21.988988176476504	28.858897093683105	27.43707529415145	21.71503943568894
52	21.8036849477427	28.774647128542185	27.467496318643825	21.954171605071295
53	21.553224233695662	28.77190178518517	27.935427332853262	21.739446648265908
54	21.480631452104586	29.041912135097203	27.827657142446665	21.649799270351544
55	21.326851658968632	28.547262109142107	27.955183783123704	22.17070244876556
56	21.605539637915847	29.049966822870044	27.72440804188489	21.62008549732922
57	21.436216349201505	28.792477090250852	27.57331748252251	22.197989078025138
58	21.204385690029657	28.321659170719926	28.290232761941365	22.183722377309056
59	21.34542620457317	29.189630371890807	27.516579660487345	21.948363763048683
60	21.262039941132578	29.334131880623765	27.688789240084084	21.715038938159577
61	22.014233840119818	28.775218104956164	27.781758559878746	21.428789495045272
62	21.971115943876864	28.95876246078366	27.83466553649387	21.23545605884561
63	21.308566795395915	28.87617658863597	28.103352138399057	21.711904477569057
64	21.62237149401591	28.601700812514647	27.648035427757673	22.127892265711775
65	21.32757594287146	28.88168167521027	27.696980961642236	22.093761420276035
66	21.39437365873807	29.184692329104152	27.759250778942075	21.661683233215705
67	21.59878315197042	28.998731252755462	27.406915884661625	21.995569710612493
68	21.45754877082963	28.853582453815196	28.187950197453844	21.50091857790133
69	21.477912396660077	29.487311873529087	27.15656845464447	21.878207275166364
70	21.748351839779613	28.908667410157086	27.804745767582023	21.53823498248128
71	21.620012660557872	28.987828804116027	27.636561176982926	21.755597358343174
72	21.683862898251167	29.782268861008177	26.934138328276674	21.59972991246398
73	21.484163854763953	29.291616010516126	27.374254524035706	21.84996561068421
74	21.489725643017163	29.40302326938704	27.250818222224215	21.856432865371577
75	21.6442100310496	29.394218561159093	27.56168167068031	21.399889737111007
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	936.0
1	1118.5
2	1408.0
3	1517.0
4	1519.0
5	1339.5
6	1124.0
7	1035.5
8	983.0
9	1310.0
10	1941.5
11	2137.5
12	2029.0
13	1878.5
14	1819.5
15	2029.0
16	2147.0
17	2365.5
18	2794.5
19	3367.5
20	3730.0
21	4249.5
22	5767.5
23	7402.0
24	8038.0
25	9255.0
26	12171.0
27	15321.0
28	16772.0
29	19198.5
30	23709.5
31	27780.5
32	29767.0
33	33385.5
34	39836.0
35	45331.5
36	47995.0
37	49315.5
38	49394.5
39	48218.0
40	48283.0
41	49982.5
42	51375.0
43	50188.5
44	49309.0
45	50481.5
46	51279.5
47	51039.5
48	51174.0
49	50473.0
50	48480.0
51	44870.5
52	42553.0
53	40621.5
54	37406.0
55	33662.5
56	31203.0
57	29957.0
58	26130.0
59	21051.0
60	18553.0
61	16837.0
62	12906.5
63	9117.0
64	7542.0
65	6324.5
66	4297.0
67	2983.0
68	2479.0
69	2163.5
70	1441.0
71	842.0
72	650.0
73	587.5
74	485.0
75	333.0
76	221.0
77	185.0
78	107.5
79	55.0
80	44.0
81	37.0
82	19.5
83	6.0
84	3.0
85	2.5
86	1.0
87	1.0
88	2.0
89	1.5
90	0.5
91	0.5
92	1.0
93	0.5
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0049383868893909085
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.09643322762192429
29	0.0
30	0.0
31	0.0
32	0.0
33	0.059440220377759666
34	8.978885253438016E-5
35	0.0
36	0.0
37	8.978885253438016E-5
38	7.183108202750412E-4
39	2.693665576031404E-4
40	0.0
41	7.183108202750412E-4
42	0.001257043935481322
43	0.0
44	6.28521967740661E-4
45	0.001257043935481322
46	0.0
47	0.0
48	8.978885253438016E-5
49	0.0
50	8.978885253438016E-5
51	0.0010774662304125617
52	3.591554101375206E-4
53	1.795777050687603E-4
54	0.0033221875437720655
55	0.001795777050687603
56	4.489442626719007E-4
57	5.387331152062808E-4
58	9.876773778781816E-4
59	8.978885253438015E-4
60	0.0031426098387033054
61	0.003591554101375206
62	0.0026936655760314045
63	0.0015264104930844628
64	0.0018855659032219834
65	0.002244721313359504
66	0.0030528209861689255
67	0.002424299018428264
68	0.004130287216581487
69	0.004040498364047107
70	0.005207753446994049
71	0.0026038767234970246
72	9.876773778781816E-4
73	5.387331152062808E-4
74	0.0026038767234970246
75	0.0019753547557563632
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
75	1113724.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.415085901943236
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.69244712555926	48.75041124728675
2	9.910776925876425	11.97520878663642
3	3.823106763099876	6.929199705149373
4	1.8521077877873289	4.47581004395318
5	1.0367603564538845	3.131798299744536
6	0.6700415226067138	2.428836968769207
7	0.4298916376722127	1.8180358152945653
8	0.30375663390939633	1.468118650473704
9	0.22176273239193647	1.205803307457764
>10	0.9523568408659138	10.102884756381576
>50	0.0659407633970908	2.747299604282253
>100	0.03983907638467344	4.5002256546484505
>500	0.0012118339954308023	0.46636715992223543
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	1.795777050687603E-4	0.0
8	0.0	0.0	0.0	1.795777050687603E-4	0.0
9	0.0	0.0	0.0	1.795777050687603E-4	0.0
10	0.0	0.0	0.0	2.6936655760314047E-4	0.0
11	0.0	0.0	0.0	3.591554101375206E-4	0.0
12	0.0	0.0	0.0	3.591554101375206E-4	0.0
13	0.0	0.0	0.0	3.591554101375206E-4	0.0
14	0.0	0.0	0.0	3.591554101375206E-4	0.0
15	0.0	0.0	0.0	3.591554101375206E-4	0.0
16	0.0	0.0	0.0	3.591554101375206E-4	0.0
17	0.0	0.0	0.0	3.591554101375206E-4	0.0
18	0.0	0.0	0.0	3.591554101375206E-4	0.0
19	0.0	0.0	0.0	4.489442626719008E-4	0.0
20	0.0	0.0	0.0	4.489442626719008E-4	0.0
21	0.0	0.0	0.0	6.285219677406611E-4	0.0
22	0.0	0.0	0.0	7.183108202750412E-4	0.0
23	0.0	0.0	0.0	9.876773778781816E-4	0.0
24	0.0	0.0	0.0	0.002334510165893884	0.0
25	0.0	0.0	0.0	0.002424299018428264	0.0
26	0.0	0.0	0.0	0.002963032133634545	0.0
27	0.0	0.0	0.0	0.002963032133634545	0.0
28	0.0	0.0	0.0	0.005387331152062809	0.0
29	0.0	0.0	0.0	0.009248251811041155	0.0
30	0.0	0.0	0.0	0.015982415751119666	0.0
31	0.0	0.0	0.0	0.032503564617445614	0.0
32	0.0	0.0	0.0	0.04624125905520578	0.0
33	0.0	0.0	0.0	0.06303177447913487	0.0
34	0.0	0.0	0.0	0.08152827810121718	0.0
35	0.0	0.0	0.0	0.100383937133437	0.0
36	0.0	0.0	0.0	0.12310051682463519	0.0
37	0.0	0.0	0.0	0.15775901390290592	0.0
38	0.0	0.0	0.0	0.202653440170096	0.0
39	0.0	0.0	0.0	0.2590408395616867	0.0
40	0.0	0.0	0.0	0.3171342271514307	0.0
41	0.0	0.0	0.0	0.3755867701513122	0.0
42	0.0	0.0	0.0	0.43089670331249036	0.0
43	0.0	0.0	0.0	0.48503938139072156	0.0
44	0.0	0.0	0.0	0.5457366457039625	0.0
45	0.0	0.0	0.0	0.607960320510288	0.0
46	0.0	0.0	0.0	0.6778160477820357	0.0
47	0.0	0.0	0.0	0.744708742920149	0.0
48	0.0	0.0	0.0	0.8115116492057278	0.0
49	0.0	0.0	0.0	0.8907952059935855	0.0
50	0.0	0.0	0.0	0.96837277458329	0.0
51	0.0	0.0	0.0	1.0533130290808135	0.0
52	0.0	0.0	0.0	1.132327219311068	0.0
53	0.0	0.0	0.0	1.213586130854682	0.0
54	0.0	0.0	0.0	1.2973591302692589	0.0
55	0.0	0.0	0.0	1.3849930503428138	0.0
56	0.0	0.0	0.0	1.4755900025500035	0.0
57	0.0	0.0	0.0	1.5604404681949926	0.0
58	0.0	0.0	0.0	1.6547187633560918	0.0
59	0.0	0.0	0.0	1.753755867701513	0.0
60	0.0	0.0	0.0	1.8565641038533784	0.0
61	0.0	0.0	0.0	1.9696980580466974	0.0
62	0.0	0.0	0.0	2.0828320122400164	0.0
63	0.0	0.0	0.0	2.1954272333181293	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1300	0.0	42.988533	1
GTATCAA	3420	0.0	40.95254	1
GACGCGT	40	0.0025991353	34.492325	20
ATCAACG	4640	0.0	29.660425	3
TATCAAC	4765	0.0	29.316666	2
TCAACGC	4680	0.0	29.185812	4
CAACGCA	4820	0.0	28.409653	5
AACGCAG	4965	0.0	27.441023	6
ACGCAGA	5640	0.0	23.91223	7
GTACATG	7285	0.0	23.818771	1
CGCAGAG	5585	0.0	23.653643	8
GAGTACT	2335	0.0	23.04412	12
ACACCGT	90	2.1295034E-4	22.994883	34
AACCGTT	165	3.1523086E-9	22.99488	34
TACATGG	7550	0.0	22.522802	2
ACGAAAT	155	4.0958184E-8	22.25311	12
CGTATAG	110	3.2690084E-5	21.952616	1
TCGCTAA	110	3.2724092E-5	21.94966	46
GTACTTT	2420	0.0	21.807129	14
AGTACTT	2585	0.0	21.61608	13
>>END_MODULE