FastQCFastQC Report
Mon 6 Feb 2017
SRR3203956_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3203956_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1113724
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30000.26936655760314043No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16160.1450987856955583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13080.11744381911496923No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19900.045.8120421
GTATCAA60350.044.752851
ATATCGG557.507777E-637.55277367
TATCAAC81850.032.766672
CGCTAGC851.1498014E-732.6547633
ATCAACG82050.032.357023
TCAACGC81950.032.29874
AGCGTAC851.3375393E-732.09705711
TATCTCG14250.031.89567836
CGCTATC14000.031.72176433
CAACGCA83250.031.5343255
AACGCAG83900.031.168116
CGCTACG17650.031.03915228
TATGCCG17100.031.02509543
ACGCTAT15000.030.76350432
CTCGTAT16700.030.51817939
TCGTATG17400.030.48596440
TACGCTA17500.030.33393731
GCTACGC18050.029.77501329
CGTATGC18350.029.66343141
Produced by FastQC (version 0.11.5)